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Novel compound microbial agent and applications thereof

A technology of compound microbial bacterial agent and bacterial agent, which is applied in the field of agricultural microorganisms to achieve a significant effect of increasing production and improving the structure of aggregates

Inactive Publication Date: 2012-12-12
临沂中磷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bacillus colloidus has the ability to utilize and decompose phosphorus and potassium minerals, but the content of available phosphorus and potassium released in a short period of time is not high, especially the ability to decompose potassium feldspar is lower, which is different from that of such rock minerals. special structure related

Method used

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  • Novel compound microbial agent and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1 prepares novel composite microbial bacterial agent

[0064] Raw material component parts by weight are as follows:

[0065] Bacillus subtilis bacterial agent 20 parts,

[0066] Jelly-like bacillus bacterial agent 30 parts,

[0067] 50 parts of Bacillus licheniformis agent.

[0068] The culture medium of the novel composite microbial bacterial agent has the following components by weight:

[0069] Sodium humate 10 parts,

[0070] Bran 35 parts,

[0071] 45 parts of rice bran,

[0072] Seaweed 10 parts;

[0073] Preparation:

[0074] 1) Cultivation of a single bacterial agent

[0075] Take out the strains preserved in the 4°C refrigerator by the test tube slant preservation method, expand them to fresh test tube beef extract peptone slant medium, cultivate them in a 30°C incubator for 2-4 days, and inoculate them in the culture medium (beef extract 5g , peptone 5g, sodium chloride 5g, pH7.2, water 1L) carry out shaker culture; Bacteria solution bac...

Embodiment 2

[0078] Embodiment 2 prepares novel composite microbial bacterial agent

[0079] Raw material component parts by weight are as follows:

[0080] Bacillus subtilis agent 15 parts,

[0081] Jelly-like bacillus bacterial agent 30 parts,

[0082] 55 parts of Bacillus licheniformis agent.

[0083] The culture medium of the novel composite microbial bacterial agent has the following components by weight:

[0084] Sodium humate 8 parts,

[0085] 45 parts bran,

[0086] 35 parts of rice bran,

[0087] Seaweed 12 parts.

[0088] The preparation method is the same as in Example 1.

Embodiment 3

[0089] Example 3: Preparation of special compound microbial fertilizer for peanuts, nitrogen, phosphorus pentoxide, and potassium oxide contents were 19%, 6%, and 13% respectively, all in mass percent.

[0090] Raw material component parts by weight are as follows:

[0091] 8 parts of new compound microbial agent,

[0092] 8 parts peat soil,

[0093] 40 parts of urea,

[0094] Calcium magnesium phosphate fertilizer 10 parts,

[0095] 8 parts of monoammonium phosphate,

[0096] Potassium sulfate 25 parts,

[0097] 1 part bentonite;

[0098] The standard of peat wood is 2-5% water content, organic matter content ≥ 30%;

[0099] Urea is small granular urea with a particle size of 0.85mm-2.80mm and a nitrogen content of 46.2%, which is a mass percentage;

[0100] In the calcium magnesium phosphate fertilizer, the content of phosphorus pentoxide is 18%, the content of calcium oxide is 45%, the content of magnesium oxide is 12%, and the content of silicon oxide is 20%, all ...

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Abstract

The invention discloses a novel compound microbial agent and relates to the technical field of agriculture microbes. 10 parts to 20 parts of bacillus subtilis, 20 parts to 30 parts of bacillus mucilaginosus and 50 parts to 70 pats of bacillus licheniformis serve as raw materials; and 5 parts to 15 parts of sodium humate, 30 parts to 50 parts of bran, 30 parts to 50 parts of rice bran and 5 parts to 15 parts of seaweed serve as raw materials for culture media. A preparation method of the novel compound microbial agent includes mixing and smashing the bacillus and maintaining the moisture to be in 30% to obtain the novel compound microbial agent. Bacterial manure prepared by mixing the novel compound microbial agent with nitrogen, phosphorus and potassium according to an appropriate ratio can effectively improve the granular structure of the soil and has a stimulation effect on the growth of crops, and the yield-increasing effect is apparent.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganisms, in particular to a novel composite microbial bacterial agent. Background technique [0002] Composite microorganisms are a type of microbial preparation products that have emerged at home and abroad in recent years. Its characteristic is that by compounding two or more microbial strains, it can play multiple functions such as enhancing crop nutrient absorption, disease resistance, and barrenness resistance. Composite microbial bacterial agents are microbial preparations made from two or more microbial strains that are not antagonistic to each other. Such microbial agents generally have excellent characteristics such as complete types, reasonable compatibility, and strong functionality. However, the stability of the compound microbial preparations currently used is not strong and is easily affected by the environment. The population structure may cause some microorganisms to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/04C12R1/125C12R1/10C12R1/07C05G3/80
Inventor 王怀欣
Owner 临沂中磷生物科技有限公司
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