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Composite microbial manure and its preparation method

A compound fertilizer and microbial technology, applied in the field of microbial compound fertilizer and its preparation, can solve the problems of research, variety of strains, high cost, etc., and achieve the effect of simple preparation process, round and beautiful appearance, and convenient source

Inactive Publication Date: 2005-08-17
TIANJIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, the vast majority of microbial compound bacteria agents simply put together several functional strains, and the research on the mutual growth and synergy between bacteria and bacteria is not in-depth.
Therefore, there are many types of bacterial strains in the product and the cost is high; at the same time, mutual antagonism between the bacterial strains in the product also occurs, which greatly reduces the activity of microorganisms.
In the process of preparing microbial fertilizer with chicken manure as the main raw material, there is still a process in which the microbial compound fertilizer does not go through granulation, which greatly affects the service life of the product, and at the same time, the active ingredients in the product cannot be well utilized.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] (1) The cultivation process of bacteria

[0017] 1. The cultivation process of nitrogen-fixing bacteria brown ball

[0018] ① Primary slant culture: The main ingredients of the medium are: 1% sucrose, 0.05% dipotassium hydrogen phosphate, 0.1% calcium carbonate, 0.02% magnesium sulfate, 0.02% sodium chloride, 0.04% yeast extract, 2% agar, pH7 ~7.2. Inoculate the nitrogen-fixing bacterium brown on the slant of the above medium, culture at 28°C for 24 hours, then make a bacterial suspension, and adjust the number of bacteria in the bacterial suspension to 10 8 individual / ml.

[0019] ②Secondary seed culture: inoculate Azotobacter sphaeroides into the primary medium with agar removed according to the inoculum amount of 1%, shake culture (shaking table rotation speed is 160r / m), culture at 25~30℃ for 48~72h , the number of bacteria measured by hemocytometer reached (3.5~6.2)×10 8 individual / ml.

[0020] 2. The cultivation process of Bacillus cereus

[0021] ①First-lev...

example 2

[0033] (1) Carry out the culture process of bacterial classification by embodiment example 1.

[0034] (2) The fermented broth of the four microbial strains was composed of a complex microbial flora fermented broth according to the ratio of Azotosphaerus phloomus: Bacillus cereus: Bacillus megaterium: Bacillus jelly-like = 25:30:15:30. Get this fermented liquid 40mL and mix with 360mL dextrin aqueous solution (wherein contain dextrin 25g), be prepared into the microbial functional inoculum containing binder.

[0035] (3) Mix the materials composed of 610g chicken manure, 150g urea, 150g calcium hydrogen phosphate, 75g potassium chloride and 100g bentonite to obtain 1085g compound fertilizer.

[0036](4) Adjust the rotating speed of the disc granulator to 26r / min, and adjust the inclination angle to 50°. Put the uniformly mixed compound fertilizer in the turntable of the disc granulator, start the granulator, spray the microbial functional bacteria agent on the material on the...

example 3

[0038] (1) Carry out the culture process of bacterial classification by embodiment example 1.

[0039] (2) The fermented broth of the four microbial strains was composed of a composite microbial flora fermented broth according to the ratio of Azotosphaerus phloomus: Bacillus cereus: Bacillus megaterium: Bacillus jelly-like = 15:30:25:30. Get 100mL of this fermented liquid and mix with 800mL dextrin aqueous solution (which contains 100g of dextrin) to prepare a microbial functional bacterial agent containing a binder.

[0040] (3) Mix the materials composed of 1600g chicken manure, 250g urea, 250g calcium hydrogen phosphate, 125g potassium chloride, and 230g bentonite to obtain 2455g compound fertilizer

[0041] (4) Adjust the rotating speed of the disc granulator to 30r / min, and adjust the inclination angle to 55°. Put the uniformly mixed compound fertilizer in the turntable of the disc granulator, start the granulator, spray the microbial functional bacteria agent on the mat...

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Abstract

The present invention discloses one kind of composite microbial fertilizer and its preparation process, and belongs to the field of applied microbial technology and fertilizer producing technology. The composite microbial fertilizer is prepared with microbial preparation, fermented chicken excrement, chemical fertilizer comprising urea, calcium biphospahate and potassium chloride in certain proportion, and bentonite, and through mixing and pelletizing. The microbial preparation consists of brown nitrogen fixing bacteria, cereour bacillus, giant bacillus and jelly-like bacillus as well as certain amount of dextrin. The present invention has the advantages of easy bacteria seed culture, simple preparation process, etc.

Description

technical field [0001] The invention relates to a microbial compound fertilizer and a preparation method thereof, belonging to applied microbial technology and fertilizer manufacturing technology. Background technique [0002] With the development of microbial technology, many functional bacteria have been applied in fertilizers, and microbial fertilizers have gradually developed from single microbial fertilizers to multifunctional compound bacterial fertilizers. However, so far, the vast majority of microbial compound bacteria agents simply put together several functional strains, and the research on the mutual growth and synergy between bacteria and bacteria is not in-depth. Therefore, there are many types of bacterial strains in the product and the cost is high; at the same time, mutual antagonism between the bacterial strains in the product also occurs, which greatly reduces the activity of microorganisms. In the process of preparing microbial fertilizer with chicken ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F3/02C05F11/08C05G1/00
CPCY02A40/20
Inventor 张毅民魏胜堂王虹张克敏高敏杰吕学斌曹增管孙亚凯
Owner TIANJIN UNIV
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