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Acinetobacter calcoaceticus and culture method and application thereof

A cultivation method, calcium acetate technology, applied in the field of microorganisms, can solve the problems of slow degradation of petroleum, secondary pollution, limited number of viable bacteria, etc., achieve obvious effects, speed up the restoration process, and improve the effect of the micro-ecological environment

Inactive Publication Date: 2010-12-15
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Wherein the physical and chemical methods have the following defects: negative treatment, high cost and serious secondary pollution
Chinese patent document announcement number CN 2623365Y (application number 03214009.6) discloses a bioremediation facility for contaminated soil, and Chinese patent document publication number CN 101234392A (application number 200810014714.2) discloses a bioremediation device and method for high-concentration petroleum-contaminated soil, These remediation methods are all ex-situ remediation methods, which are suitable for heavily polluted soils, with high investment costs, and are not suitable for slightly polluted and large-area soils.
The production of these microbial agents is mostly used directly after microbial liquid fermentation or after adsorption with solid microbial carriers. The oil-degrading bacteria in the agent only come from the liquid fermentation broth, the number of viable bacteria is limited, and the bacteria themselves degrade petroleum. The speed is slow, and the production cost of the bacterial agent is high

Method used

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  • Acinetobacter calcoaceticus and culture method and application thereof

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specific Embodiment approach

[0034] The present invention will be further described below in conjunction with the examples, but the protection scope of the present invention is not limited thereto.

[0035] Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) WG-6 described in the examples was deposited in the General Microbiology Center of China Committee for the Collection of Microbial Cultures on June 11, 2010, with a preservation number of CGMCC No.3915.

[0036] The peat described in the examples was purchased from Guanghe Garden Group, the bran was purchased from Dongjia Flour Mill in Licheng District, Jinan City, and the crude oil was from the dehydrated crude oil in Gudong Oilfield, Shengli Oilfield. Other reagents used in the examples were commercially available common products.

Embodiment 1

[0037] Embodiment 1: the degradation of petroleum by Acinetobacter calcoacetate

[0038] Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) WG-6 was picked and inoculated on beef extract peptone slant medium, and activated for 16 hours.

[0039] The components of the beef extract peptone slant medium are as follows: 3 g beef extract, 5 g peptone, 5 g NaCl, 1.5 g agar, 1000 mL distilled water, pH 7.2.

[0040] Pick Acinetobacter calcoaceticus from the beef extract peptone slant medium and connect it to the liquid beef extract peptone medium, cultivate it on a shaker with a rotation speed of 250rpm at 28°C for 24 hours, and then inoculate it into crude oil culture according to the volume ratio of 5% inoculum Medium, cultured on a shaker at 28°C for 7 days with a rotation speed of 150rpm. The crude oil culture medium without bacteria was used as blank control. At the end of the cultivation, add chloroform to the medium for extraction, heat the extract to 75°C to volatilize...

Embodiment 2

[0047] Embodiment 2: the preparation of the high-density microbial agent that can remediate petroleum-contaminated soil

[0048] Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) WG-6 culture:

[0049] (1) Inoculate the Acinetobacter calcoaceticus (Acinetobacter calcoaceticus) WG-6 with the strain preservation number of CGMCC No.3915 on the beef extract peptone slant medium (the components are the same as in Example 1), and the culture temperature is 28°C , activated for 24 hours, and then transferred to liquid beef extract peptone medium at a culture temperature of 20°C and activated for 16 hours in a shaker flask to obtain the activated bacterial liquid;

[0050] (2) the bacterium liquid after the activation that step (1) is made is transferred in the liquid fermentation medium in the ratio of 5% by volume, shaker cultivates 16h, and rotating speed is 200rpm, and cultivation temperature is 20 ℃, obtains calcium acetate not Acinetobacter bacteria liquid. After testi...

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Abstract

The invention relates to an Acinetobacter calcoaceticus WG-6 capable of degrading oil and a culture method and application thereof, and belongs to the technical field of microorganisms. The bacterial strain has been collected in a China General Microbiological Culture Collection Center on June 11th, 2010 with a collection number of CGMCC No.3915. The Acinetobacter calcoaceticus can directly decompose oil contaminants into a nutrient material for utilization and growth and reproduction, and has remarkable oil degradation effect. The invention also provides a high-density microbial inoculum prepared by mixing the Acinetobacter calcoaceticus and other components, the microbial inoculum can rapidly decompose the oil, and the other components in the microbial inoculum can improve the microecological environment of soil, and accelerate the soil mediation progress.

Description

technical field [0001] The invention relates to a strain of Acinetobacter calcoaceticus WG-6 capable of degrading petroleum, a cultivation method and application thereof, and belongs to the technical field of microbes. Background technique [0002] As a big oil producing and consuming country, our country is relatively backward in terms of production conditions and environmental protection technology, and the problem of oil pollution is quite prominent. After petroleum pollutants enter the soil, due to their special physical and chemical properties, they remain for a long time and are difficult to remove, causing a series of harm to the soil environment and causing serious damage to the ecosystem, groundwater sources and human health in polluted areas. negative impact. [0003] The treatment methods of petroleum pollution in soil can be divided into three types according to their properties: physical methods, chemical methods and biological methods. Wherein the physical an...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10A62D3/02C12R1/01A62D101/20
Inventor 高永超胥九兵祝威王加宁桂召龙张强董健陈贯虹王田丽孔学刘娜李艳
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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