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Bacillus coagulans, preparation of high-density cultivated spore preparation, and use thereof

A technology of Bacillus coagulans and spores, applied in the field of microorganisms, can solve the problems of unfavorable feed processing and storage, affecting quality and application effect, poor stress resistance, etc., to improve animal immunity and resistance, wide temperature growth range, improve The effect of immunity

Active Publication Date: 2009-04-22
江苏省苏微微生物研究有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Since the biological characteristics of the strain itself are the key factors affecting the application effect of lactic acid bacteria feed microbial additives, most lactic acid bacteria do not produce spores, and the vegetative bacteria have poor environmental stress resistance, oxygen resistance, and heat resistance, which is not conducive to feed processing and production. Preservation seriously affects its quality and application effect, and its application in aquaculture and feed industry is greatly restricted

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the cultivation of high-density Bacillus coagulans spores

[0035] 1. Activation of bacteria

[0036] Aseptically open the freeze-dried strain of Bacillus coagulans JSSW-LA-07, streak and inoculate it on the slant of bran nutrient agar in a test tube, the composition of the slant medium is: peptone 10g / L, beef extract 3g / L, NaCl 5g / L, Bran 10g / L, agar 15-20g / L, distilled water 1L, pH 7.0-7.2. Culture at 40°C for 20-24 hours, then transfer to the slant of bran nutrient agar eggplant bottle, culture at 40°C for 20-24 hours , Microscopic examination, when more than 90% of the bacteria form spores, it is mature and ready for transplantation.

[0037] 2. 100L seed tank cultivation

[0038] Prepare a sterile triangular flask with glass beads inside, scrape and wash the mature sludge on the slope of the eggplant bottle with sterile water, put it into the triangular flask, shake and disperse the sludge to obtain a uniform bacterial suspension. Heat the bacteria...

Embodiment 2

[0047] Example 2: Static culture of Bacillus coagulans to produce L-lactic acid

[0048] 1. Static fermentation culture

[0049] Insert Bacillus coagulans spores into the following medium, the medium consists of: peptone 5g / L, yeast extract 5g / L, glucose 10g / L, beef extract 1g / L, Tween-80 0.5g / L, Distilled water 1000mL, pH7.0.

[0050] Culture conditions: culture temperature: 45°C, culture time: 72h, static anaerobic culture.

[0051] 2. L-lactic acid detection

[0052] Heat the fermentation broth to 80-100°C first, then centrifuge at 4000rpm / min for 5 minutes, take the supernatant and measure the L-lactic acid content with a SBA-40C biosensor analyzer, and the measured L-lactic acid content is 8.5g / L .

Embodiment 3

[0053] Embodiment 3: making probiotics

[0054] 1, the cultivation method of bacillus coagulans spores is identical with that described in embodiment 1

[0055] 2. Preparation of Bacillus coagulans powder

[0056] Mix the wet bacteria of Bacillus coagulans collected by high-density pure culture with dry starch 1:1-5, dry at 40-50°C for 20-24h, pulverize with a pulverizer, pass through a 40-mesh sieve, and finally adjust the bacterial powder with dry starch The number of spores in the bacteria powder, so that the number of spores of Bacillus coagulans in the bacteria powder is ≥5×10 9 pcs / g.

[0057] 3. Finished product preparation

[0058] Take the auxiliary material stone powder and pass it through a 40-mesh sieve, mix it with Bacillus coagulans powder, and mix well with a feed mixer, so that the total number of Bacillus coagulans spores in the finished product is ≥ 1×10 9 pcs / g. The finished product is packed in aluminum foil bags, each bag contains 1kg, 15 bags are pac...

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Abstract

The present invention discloses a preparation method and application of Bacillus coagulans and a spore preparation cultured in high density, and belongs to the microbe technical field. A Bacillus coagulans strain is classified and named Bacillus coagulans JSSW-LA-07 and is preserved as CGMCC No.2602 in China General Microbiological Culture Collection Center. The Bacillus coagulans strain JSSW-LA-07 can produce spore under an aerobic condition and produce a small amount of L-lactic acid in the metabolism during a course of fermentation. The Bacillus coagulans strain can produce L-lactic acid with a higher purity under an anaerobic condition. The strain does not produce substances which can be harmful to human and cultured animals, has the characteristics of innocuity, on residue, no side effect and the like, has a great significance for production of green and safe livestock, fowls and marine products and improvement of cultivation ecological environment, and can be widely applied in the cultivation production of high yield, high quality, high efficiency, synusiologic and safe livestock, fowls and marine products, and the regulation and control of the ecological environment of cultivation.

Description

technical field [0001] The preparation and application of a bacillus coagulans and a high-density cultured spore preparation belong to the technical field of microorganisms. Background technique [0002] my country is a large agricultural country, and aquaculture occupies a considerable proportion in the whole agriculture. With the rapid development of animal husbandry and aquaculture in my country, the output of meat and poultry has leapt to the forefront of the world. However, the rapid development of aquaculture economy has neglected Food safety of livestock, poultry and aquatic products. In recent years, high-density intensive artificial breeding has been developed in a way that emphasizes quantity over quality, resulting in the destruction of the breeding ecological environment and frequent outbreaks of breeding animal diseases; people have used antibiotics and chemically synthesized drugs in large quantities for a long time, leading to drug resistance and drug resistanc...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P7/56C02F3/34A23K1/16C12R1/07A23K10/18
Inventor 匡群孙梅陈秋红施大林夏冬何义进
Owner 江苏省苏微微生物研究有限公司
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