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Bacillus velezensis for control of cucumber fusarium wilt and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of development and utilization of microbial germplasm resources, can solve problems such as polluting the environment and threatening human physical and mental health, reduce pesticide pollution, avoid pathogenic bacteria resistance, and control effect of effect

Active Publication Date: 2018-04-20
INST OF PLANT PROTECTION FAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cucumber Fusarium wilt is a typical soil-borne disease. Symptoms do not appear in the early stage of pathogenic bacteria infection, and symptoms appear in the middle and late stages. At present, chemical fungicides are still the main method of disease control. A large number of chemical pesticides are used for a long time and repeatedly, which seriously pollutes the environment. Threat to human physical and mental health, causing pathogenic bacteria to gradually develop resistance and then mutate to produce many physiological races

Method used

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  • Bacillus velezensis for control of cucumber fusarium wilt and application thereof
  • Bacillus velezensis for control of cucumber fusarium wilt and application thereof
  • Bacillus velezensis for control of cucumber fusarium wilt and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1 Preparation of biocontrol agent containing FJ17-4 bacterial strain

[0022] 1. Acquisition and identification of FJ17-4 strain

[0023] Bacillus Velez FJ17-4 was isolated from healthy cucumber rhizosphere soil in Yunlong Township, Minqing County, Fuzhou City, Fujian Province. The bacteria in the collected soil samples were separated by plate dilution method, and the medium used was LB ( tryptone 10 g, yeast extract 5 g, sodium chloride 5 g, agar 15 g, water 1000 mL, pH 7.2). According to different colony morphology, the isolated bacteria were purified, and a total of 326 strains of bacteria were isolated and purified. Then, with Phytophthora cucumber as the target pathogen, the plate confrontation culture method was adopted ( Figure 4 ) to measure the antagonistic ability of the purified bacteria to Phytophthora cucumber, and obtained 18 bacterial strains with antagonistic effect on Phytophthora cucumber. Through the indoor pot test, the disease prevent...

Embodiment 2

[0041] Embodiment 2: the antagonistic activity of FJ17-4 bacterial strain to cucumber wilt pathogen

[0042] Using the confrontation culture method, inoculate the Fusarium wilt of cucumber stored at 4°C on a PDA plate for activation, and after the fungus is overgrown on the plate, use a sterilized hole puncher to uniformly punch out a circle with a diameter of 5 mm from the outer edge of the colony Bacteria. Inoculate the mycelium block on the center of the improved NB medium plate (5g peptone, 10g glucose, 3g gravy extract, 5g sodium chloride, 20g agar, 1000mL distilled water, pH=7.2), and place them around 25mm away from the center respectively. FJ17-4 was inoculated, and water was used as the control group in the test. Cultivate at 25°C, observe and record the size of the inhibition zone after the mycelia cover the plate.

[0043] The activity of FJ17-4 against Fusarium wilt of cucumber was evaluated according to the size of the inhibition zone: 0 mm, no inhibition; 5 mm,...

Embodiment 3

[0045] Embodiment 3: The control effect of FJ17-4 bacterial strain to cucumber wilt

[0046] 1) Control effect of indoor pot test

[0047] Sow the cucumber variety Jinyan No. 4 in the seedling tray, and leave one plant in each hole after emergence. When the cucumber seedlings grow to 4 true leaves, pull up the cucumber seedlings, shake the root soil as much as possible, and then soak the roots in The concentration of conidia was 1×10 8 After 5min in the Cucumber wilt spore suspension in / mL, the cucumber seedlings inoculated with Cucumber wilt were obtained, and the inoculated cucumber seedlings were transplanted in flower pots with an inner diameter of 90mm, and 2 plants were planted in each pot. After 24 hours, , the FJ17-4 strain fermentation liquid (cell concentration of 1 × 10 10 CFU / mL) was poured on the roots of cucumber seedlings inoculated with cucumber wilt, 50 mL per plant, and 1000 times of 30% hymexazol water was used as the treatment control, that is, one group...

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Abstract

The present invention provides Bacillus velezensis for control of cucumber fusarium wilt and application thereof. The Bacillus velezensis is coded as FJ17-4, and the registration number is CGMCC No. 14642 in the China General Microbiological Culture Collection Center. The Bacillus velezensis FJ17-4 has a significant antagonism effect on cucumber fusarium wilt-Fusarium oxysporum f.sp cucumerinum, and can effectively control cucumber fusarium wilt, and the control effect is stable. The Bacillus velezensis provides an excellent bacterial strain resource for biological control of the cucumber fusarium wilt.

Description

technical field [0001] The invention relates to the field of development and utilization of microbial germplasm resources, in particular to a strain of Bacillus Velez for preventing and treating cucumber wilt and its application. Background technique [0002] Cucumber specialization by Fusarium oxysporum ( Fusarium oxysporum f. sp. Cucumerinum Owen) infection, is one of the most difficult soil-borne systemic diseases in cucumber production. The disease has the characteristics of rapid onset, severe disease, and rapid spread. Once it occurs, it often causes huge economic losses. The primary source of infection of the disease is mainly the soil with bacteria, diseased plant residues, seeds and manure, etc. The bacteria spread from the rotten host tissue to the soil and can live in rot, and the survival time is as long as 5-6 years or longer time. Fusarium wilt of cucumber can be spread over long distances with the help of rainwater, irrigation water, etc., and can occur t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01G13/00C12R1/07
CPCA01G13/00A01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 兰成忠游泳杨秀娟阮宏椿
Owner INST OF PLANT PROTECTION FAAS
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