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Bacillus velezensis ZF2 and application thereof to control of plant diseases

A technology of ZF2 and Bacillus, applied in the fields of application, plant growth regulator, and microbial-based methods, can solve the problems of high dependence on chemical control, lack of disease-resistant varieties, and reduced sensitivity

Active Publication Date: 2018-12-21
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, due to the lack of disease-resistant varieties, the dependence on chemical control in production is increasing, which reduces the sensitivity of pathogenic bacteria to some commonly used fungicides to a certain extent, and the control effect decreases while causing pesticide pollution of fruits.

Method used

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  • Bacillus velezensis ZF2 and application thereof to control of plant diseases
  • Bacillus velezensis ZF2 and application thereof to control of plant diseases
  • Bacillus velezensis ZF2 and application thereof to control of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Embodiment 1, the separation, purification and identification of biocontrol bacillus ZF2

[0065] 1. Isolation of biocontrol bacillus

[0066] Rinse the cucumber plants with water, disinfect the surface with 1% sodium hypochlorite aqueous solution for 1 min, and then rinse the cucumber plants with sterile water for 3 times. Put about 2g of the surface-sterilized cucumber plant into a sterilized mortar, add a small amount of sterilized quartz sand and 10ml of sterile water for full grinding, take 1ml of the grinding juice, add it to a test tube containing 9ml of sterile water, and mix After homogeneity, serially dilute to 10 -6 concentration, will be 10 -4 、10 -5 、10 -6 The concentration of bacterial solution was maintained in a water bath at 80°C for 30 minutes to kill most of the non-bacillus bacteria. Take 100 μl of LB solid plates, apply 3 plates for each concentration, and incubate at 28°C. After culturing for 1 day, different single colonies were picked and st...

Embodiment 2

[0089] Embodiment 2, the functional research of bacterial strain ZF2

[0090] 1. Application of strain ZF2 in antibacterial

[0091] 1) Determination of fungal inhibition spectrum

[0092] The method refers to the screening of antagonistic bacteria in Example 1.

[0093] 2) Determination of bacterial inhibition spectrum

[0094] The antibacterial spectrum test of pathogenic bacteria adopts the method of double-layer culture. The strain ZF2 was inoculated in liquid LB medium, and cultured with shaking at 28°C for 16 hours to obtain a ZF2 bacterial suspension. In the center of a 90 mm glass PDA plate, a hole was punched with a 10 μl pipette tip, and 5 μl of ZF2 bacterial suspension was placed in it, and incubated at 28° C. for 24 hours. Invert the Petri dishes, add 3ml of chloroform to each Petri dish in a fume hood, and ventilate for 12h. The pathogenic bacteria were shaken and cultured in NB medium at 28°C for 48 hours, 100 μl of bacterial suspension was added to 5 ml of ...

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Abstract

The invention discloses bacillus velezensis ZF2 and application thereof to control of plant diseases, and provides the Bacillus velezensis (Bacillus velezensis ) ZF2 with the preservation number of CGMCC NO.16013. The invention further relates to the bacillus, or a culture solution or bacillus suspension thereof or application of fermentation products of the bacillus to restrain bacteria. The bacterial strain ZF2 is simple in culture condition in the fermentation process, and massive active substances are generated, are secreted outside cells, and have remarkable antagonism effects on 6 disease fungi and 7 pathogenic bacteria. During pot culture test in green houses, the prevention effect of the bacterial strain ZF2 on corynespora cassiicola is as high as 92.24%, test proves that the bacillus velezensis ZF2 has good application prospects and also provides a certain theoretical basis and technical support for development and application of microbial inoculum.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a bacillus Velez ZF2 and its application in plant disease control. Background technique [0002] Corynespora leaf spot of cucumber is one of the important diseases of cucumber in greenhouse cultivation, which is mainly caused by Corynespora cassiicola infection. Corynespora multiprimum mainly infects the leaves of host plants, forming typical lesions, and can also infect roots, stems, flowers and fruits. In severe cases, it will cause leaf and fruit drop. At present, due to the lack of disease-resistant varieties, the dependence on chemical control in production is increasing, which reduces the sensitivity of pathogenic bacteria to some commonly used fungicides to a certain extent, and the decrease in control effect also causes pesticide pollution of fruits. At present, biological control is considered to be an important control method with the most development potential...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/42C12N9/54A01N63/00A01N63/02A01P1/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N9/2437C12N9/54C12N1/205C12R2001/07
Inventor 李进一魏芸
Owner BEIJING UNIV OF CHEM TECH
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