44 results about "Corynespora cassiicola" patented technology

The invention discloses an LAMP primer group and a detection method for rapidly detecting corynespora cassiicola. A set of specific detection primer group is designed and selected for specific toxin cassiicolin gene sequence of cucumber corynespora cassiicola. The primer group consists of four specific primers, i.e. CC-FIP, CC-BIP, CC-F3 and CC-B3. The invention relates to a loop-mediated isothermal amplification (LAMP) detection method containing the primer group. The detection result can be directly observed by virtue of eyes or the color variation of SYBR GreenI is used as a judging standard to determine whether the corynespora cassiicola exists in a to-be-detected sample. The selected primer is high in sensitivity and specificity. The established detection method has advantages of good accuracy, short detection time and simple instrument and device; the process from the sample treatment to the report result can be completed only in 1.5h, a PCR instrument and an electrophoresis apparatus are not needed, the operation is simple, and compared with other PCR methods, the specificity is higher.

Disclosed are a bactericide composition, a bactericide pesticide and an application of the bactericide composition and the bactericide pesticide. The bactericide composition is composed of difenoconazole and cuppric nonyl phenolsulfonate, and the weight ratio between the difenoconazole and the cuppric nonyl phenolsulfonate is in the range of (1:50)-(50:1), preferably (1:25)-(25:1). The composition is combined with a helper component which is receptive in the pesticide to compose the bactericide pesticide; and the bactericide pesticide has obvious synergism effects on preventing diseases of watermelon anthracnose, tomato early blight, celery leaf spot or Corynespora cassiicola of cucumbers, the application amount of the single bactericide composition or the single helper component can be reduced, the lasting time of the bactericide pesticide can be prolonged, the generation of insecticide resistance of microbe diseases can be postponed, and the popularization values of the bactericide pesticide are obvious.

The invention discloses a corynespora cassiicola succinodehydrogenase subunit c N75S resistance mutation detection primer and a detection method and belongs to the technical field of resistance mutation detection. The primer comprises 2 outer primers and 2 inner primers, and nucleotide sequences of the 2 outer primers and the 2 inner primers are shown as SEQ ID No. (sequence identifier number) 1,SEQ ID No. 2, SEQ ID No. 5 and SEQ ID No. 4. The four primers identify 6 specific sequence regions of a target sequence, and high specificity of LAMP (loop-mediated isothermal amplification) is ensured. The detection method comprises the steps of extracting DNA (deoxyribonucleic acid) of a sample for LAMP reaction, performing electrophoresis on a reaction product or adding a fluorescent dye for direct observation to achieve quick detection of subunit c N75S resistance mutation. The method has strong detection specificity for the N75S mutation on a subunit c, has the characteristics of simplicity, convenience, quickness, sensitiveness and stability, and is low in instrument requirements, easy and simple to operate, low in cost and easy to popularize and use at the grass-roots.

The invention discloses a corynespora cassiicola disease-resistant linkage molecular marker as well as a special primer and application thereof. The molecular maker is one of nucleotide sequences amplified out of the total DNA of a cucumber by using the following primers: SEQ ID NO:1 and SEQ ID NO:2 in the sequence table and SEQ ID NO:3 and SEQ ID NO:4 in the sequence table. The molecular marker can be used for identifying the existence of a target gene namely a corynespora cassiicola disease-resistant gene,Cca to realize indirect selection of disease-resistant plants; and the molecular marker cannot be influenced by other gene effects and environmental factors, so that the molecular marker can be used for early generation selection, the breeding time is shortened, the breeding efficiency is improved, and a technical support is provided for performing corynespora cassiicola disease-resistant breeding.

The invention discloses Streptomyces having an inhibiting effect on Corynespora cassiicola and application of the Streptomyces. The Streptomyces is Streptomyces phaeoluteichromatogenes HEBRC-45958, and the preservation number of the Streptomyces phaeoluteichromatogenes is CCTCC No. M2015768. The Streptomyces has the advantages that the Streptomyces phaeoluteichromatogenes can generate fermentation broth with high antibacterial activity after fermentation, and the fermentation broth is good in preventing and treating effect on Corynespora leaf spot caused by the Corynespora cassiicola; compared with current chemically-synthesized bactericide, the Streptomyces phaeoluteichromatogenes comes from the natural environment and is efficient, low in toxicity, free of environment pollution, less prone to tolerance generating, and the like.

The invention discloses Corynespora cassiicola resistant and suspectible gene fragments and application thereof. The Corynespora cassiicola resistant gene fragment is a nucleotide sequence of SEQ ID No.1; and the Corynespora cassiicola suspectible gene fragment is a nucleotide sequence of SEQ ID No.2. The germ plasm resource of the Corynespora cassiicola resistant gene can be screened by the Corynespora cassiicola resistant gene fragment and the Corynespora cassiicola suspectible gene fragment. Resource resistance screening in cucumber cultivation is of great practical significance. With the sequences as basis, marker-assisted selection and breeding of Corynespora cassiicola resistance can be carried out, and the gene fragments can be used as probes of inspecting Corynespora cassiicola resistant gene in cucumber breeding; the gene fragments also can be used for positioning, constructing and cloning the Corynespora cassiicola, and establishes foundation for improving disease resistanceof cucumber varieties through gene engineering.

The invention discloses a phytopathogen antagonistic bacterium and its application in plant disease control. The phytopathogen antagonistic bacterium refers to paenibacillus (Paenibacillus sp.) CEB33 CGMCC No.12719. The phytopathogen antagonistic bacterium, namely the paenibacillus (Paenibacillus sp.) CEB33 CGMCC No.12719 can control plant diseases caused by cassava xanthomonas axonopodis (Xanthomonas axonopodis pv.manihotis), cassava corynespora cassiicola (Corynespora cassiicola), cassava colletotrichum gloeosporioides (Colletotrichum gloeosporioides) and cassava phytophthora palmivora (Phytophthora palmivora); especially, the phytopathogen antagonistic bacterium and cassava can coexist, and the phytopathogen antagonistic bacterium stably survives in the cassava for controlling cassava xanthomonas axonopodis.

The invention discloses a metalaxyl-M and propamocarb hydrochloride compounded aqueous solution and an application thereof. The metalaxyl-M and propamocarb hydrochloride compounded aqueous solution is prepared from, by weight, 1-20% of metalaxyl-M, 1-60% of propamocarb hydrochloride, 3-30% of thymol, 5-20% of a surface active agent, 0.1-2% of stabilizer, 0.1-0.3% of defoamer and the balance of water. The metalaxyl-M and propamocarb hydrochloride compounded aqueous solution is efficient, safe and environment friendly, synergistic effect on the component is achieved, crop output can be increased, quality of fruit is improved, cost is lowered and generation of drug resistance to bacteria is beneficially suspended; the metalaxyl-M and propamocarb hydrochloride compounded aqueous solution can be used for preventing and controlling crop diseases of citrus burning disease, corynespora cassiicola, pepper phytophthora blight, rice seedling blight and rice blast, plasmopara viticola and the like.