Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Antibacterial activity and application of bacillus siamensis

A technology of bacillus and bacterial suspension, applied in the field of microorganisms, can solve problems such as decline and quality reduction of agricultural and sideline products

Active Publication Date: 2021-06-11
ANHUI AGRICULTURAL UNIVERSITY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problem of a large number of production and quality declines in agricultural and sideline products caused by plant pathogens

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Antibacterial activity and application of bacillus siamensis
  • Antibacterial activity and application of bacillus siamensis
  • Antibacterial activity and application of bacillus siamensis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1, the preparation of Bacillus siamensis (Bacillus siamensis) bacterial suspension

[0015] Inoculate a single colony of purified Bacillus siamensis in LB liquid medium, culture at 28°C and 180r / min for 24 hours, use a McFarland turbidimetric tube for comparison, and resuspend the bacteria in sterile water to a concentration of 1.0×10 8 cfu mL -1 spare.

Embodiment 2

[0016] Embodiment 2, bacillus siamese potted activity experiment

[0017] Potted Antibacterial Experiment

[0018] Cucumber seeds were disinfected (soaked in 2% sodium hypochlorite for 10 minutes, then sterilized with 75% alcohol for 0.5 minutes), and finally rinsed with sterile water for 3 times to remove residual components. After disinfection, the seeds were evenly sown in sterile soil (sterilized at 121°C for 20 minutes), kept moist with plastic wrap, and cultivated in a light incubator at 28°C. The soil formula is matrix soil: vermiculite: organic fertilizer = 2:2:1.

[0019] Take 1mL of sterile water in a 1.5mL centrifuge tube. Use an inoculation loop to dip a small amount of bacteria into a centrifuge tube and mix well to prepare a bacterial suspension. Use a turbidimetric tube to adjust the concentration of the bacterial suspension to 1.0×10 8 cfu mL -1 . Fusarium wilt of cucumber was inoculated into PDA medium and cultured at a constant temperature of 28°C for 7...

Embodiment 3

[0031] Embodiment 3, bacillus siamese are to the activity of 9 kinds of pathogenic bacteria

[0032] Nine species of plant pathogenic fungi tested: Corynespora cassiicola, F.oxysporum f.sp. cucumerinum, F.oxysporum f.sp. vasinfectum, Bitter melon wilt (F.oxysporum f.sp.momordicae), Alternaria solani, Botrytis cinerea, Colletotrichum graminicola, Curvularia lunata, Red wheat Mildew fungus (F. graminearum).

[0033] The inhibitory effect of Bacillus on different plant pathogenic fungi was determined by plate confrontation method. Plant pathogenic fungi were inoculated into PDA medium for activation. Incubate at 28°C for 4 days. Bacillus was inoculated in LB liquid medium and cultured at 28°C and 180r / min for 24h. The pathogenic fungi were punched into a cake with a hole punch with a diameter of 6mm and inoculated to the center of the PDA plate. Use a pipette gun to inoculate 1 μL of bacterial solution at a distance of 2 cm, inoculate a plate three times to form an equilater...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a bacillus siamensis strain and application thereof. The bacillus siamensis provided by the invention has good effects on biological control of plant pathogenic fungi such as corynespora cassiicola, cucumber fusarium wilt, cotton fusarium wilt, bitter gourd fusarium wilt, alternaria solani, botrytis cinerea, corn anthracnose, curvularia lunata and Gibberella zeae and promotion of growth of cucumber seedlings and the like, and has high application value.

Description

technical field [0001] The invention mainly relates to the technical field of microorganisms, in particular to the application of a strain of bacillus siamese in preventing and treating cucumber wilt. Background technique [0002] In my country, disasters caused by plant pathogens are an important reason for the decline in the output and quality of agricultural and sideline products in our country. The traditional method of preventing and controlling plant pathogenic bacteria is mainly to spray chemical pesticides. However, a series of problems caused by the long-term use of chemical pesticides, such as pesticide residues and environmental pollution, have attracted increasing attention. [0003] It is particularly urgent for us to find new and environment-friendly means of prevention and control. Therefore, the development of efficient, safe and pollution-free green pesticides has become one of the effective ways to solve the above problems. Currently, there are two types...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00A01G22/05C12R1/07
CPCC12N1/20A01N63/22A01G22/05
Inventor 张应烙包礼云汪军勇周凌峰吴菲
Owner ANHUI AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com