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Bacillus subtilis HMB26553 and application thereof

A technology of Bacillus subtilis and strains, applied in the field of biological control, can solve the problems of affecting the growth and development of cotton, poor chemical control effect, high drug resistance and cost, and achieve difficult drug resistance, good drug effect persistence, and good inhibitory effect. Effect

Active Publication Date: 2017-07-11
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cotton blight seriously affects the growth and development of cotton, and often leads to the death of cotton seedlings after the onset, which is an urgent problem to be solved
[0003] Cotton blight is mainly controlled chemically, such as using chemical agents for seed dressing, coating or soaking before sowing, and spraying and root irrigation with chemical agents after sowing. Long-term medication is prone to problems such as drug resistance and high cost

Method used

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  • Bacillus subtilis HMB26553 and application thereof
  • Bacillus subtilis HMB26553 and application thereof
  • Bacillus subtilis HMB26553 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] The preparation of embodiment 1HMB26553 microbial bacterial agent

[0050] Proceed as follows:

[0051] (1) Strain activation: Bacillus subtilis (Bacillus subtilis) strain HMB26553 (HMB26553) stored at -80°C was deposited in the General Microbiology Center of China Committee for Microbial Culture Collection on October 28, 2016, and the preservation number is CGMCC No.13211) is activated (30°C) on LB plate medium (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL), pick A single colony was cultured at 30°C for 12 hours on LB slant medium (its composition and weight ratio: tryptone 10g, yeast extract 5g, sodium chloride 5g, agar powder 15g, water 1000mL) to obtain activated strains;

[0052] (2) Preparation of seed liquid: make LB liquid medium according to conventional methods (its composition and weight ratio are: tryptone 10g, yeast extract 5g, sodium chloride 5g, water 1000mL), and put it in a 250...

Embodiment 2

[0055] Embodiment 2 HMB26553 bacterial strain of the present invention is to the inhibitory action test of cotton solitaire mycelia growth

[0056] (1) R. solanacearum RH-2 tested: R. solanacearum RH-2 strain was collected from the diseased strain of cotton blight in Qiu County, Handan City, Hebei Province. It was isolated and purified by the Institute of Plant Protection, Hebei Academy of Agriculture and Forestry Sciences. It was identified as Rhizoctonia solani by the Department of Plant Pathology, School of Plant Protection, University, and the pathogenicity test showed strong pathogenicity.

[0057] (2) Test method:

[0058] Plate assay test: first activate and cultivate Rhizoctonia solani RH-2 on a PDA plate for 3 days, and then use a puncher to Punch holes to make bacterial flakes, transfer the flakes of R. solani solanum to the center of another PDA plate, then spot the activated Bacillus subtilis HMB26553 at a place 2.0 cm away from the flakes of the indicator bacter...

Embodiment 3

[0063] Embodiment 3 The comparative test of the control effect of HMB26553 bacterial strain of the present invention to cotton blight

[0064] (1) Test treatment:

[0065] (1) Microbial agent: the HMB26553 liquid preparation prepared in Example 1 was diluted 50 times with water.

[0066] (2) Blank control: clear water

[0067] (2) Test method:

[0068] Mix vermiculite and cotton field soil at a ratio of 1:1, then sterilize at 121°C for 1 hour, and then sterilize again under the same conditions the next day. Inoculate Rhizoctonia solani RH-2 mycelia section suspension (about 10 in 500 gram soils) 7 per milliliter) 10 milliliters, stir evenly, fill in a plastic flower pot with a diameter of 20 cm, and compact it. The cotton variety Jifeng 106 was selected, and the seeds were soaked with the 50-fold water dilution of the HMB26553 liquid preparation prepared in Example 1 for half an hour before sowing; the seeds were soaked in clear water for half an hour as a blank control. ...

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Abstract

The invention discloses Bacillus subtilis strain HMB26553 (Bacillussubtilis), the strain is preserved in China General Microbiological Culture Collection Center, and the preservation number is CGMCCNo.13211. The present invention also discloses a microbial agent produced by use of the strain and application thereof in the prevention and control of plant diseases such as cotton damping-off and the like. The Bacillus subtilis HMB26553 has high control efficiency on the cotton damping-off, the average control efficiency is above 90%; the microbial agent has good effect perdurability, and has no environmental pollution problems; in addition, the HMB26553 has a wide antibacterial spectrum, and has a good inhibitory effect on cotton rhizoctonia solani kuh, cotton verticillium dahlia, cotton Fusarium oxysporum, tomato botrytis cinerea and cucumber Corynespora cassiicola.

Description

technical field [0001] The invention belongs to the field of biological control, and in particular relates to a bacillus subtilis HMB26553, microbial agents produced by the bacillus subtilis, and their application in preventing and treating cotton blight and other diseases. Background technique [0002] Cotton blight is a cotton seedling disease caused by Rhizoctonia solani Kühn. Its main symptoms are: cotton seeds are infected before germination and cause rotten seeds, and cotton seeds germinate before they emerge. Infected to cause rotten buds; Cotton seedlings are damaged after being unearthed, and yellow-brown lesions are formed near the base of the soil surface at the initial stage, and the lesions gradually expand and surround the entire base, showing obvious constriction, and the diseased seedlings wilt, fall and die; The cortex remains in the soil, and only the sharp rattail-shaped xylem remains; after the cotyledons are damaged, yellow-brown irregular-shaped lesions...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 马平李社增鹿秀云郭庆港张晓云王莹王培培赵卫松
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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