Brown spot resistant, sensitive gene fragment of cucumber and uses thereof

A gene fragment, anti-brown spot technology, applied in application, genetic engineering, plant genetic improvement and other directions, can solve the problems of lack of systematic research and reports on the genetic law of cucumber brown spot resistance

Inactive Publication Date: 2010-09-29
TIANJIN RES INST OF VEGETABLE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to domestic and foreign literature, so far, there is still a lack of systematic research and reports on the genetic law of resistance to cucumber brown spot and the research on molecular marker-assisted breeding.

Method used

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  • Brown spot resistant, sensitive gene fragment of cucumber and uses thereof
  • Brown spot resistant, sensitive gene fragment of cucumber and uses thereof
  • Brown spot resistant, sensitive gene fragment of cucumber and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Using the BSA method, the resistant material Q5 and the susceptible material P57-1 of cucumber brown spot and its 241 strains F 2Genomic DNA of cucumber was extracted by CTAB method. PCR amplification was carried out with 111 pairs of SSR primers and 29 pairs of EST-SSR primers, according to the specific DNA fragments in F 2 The performance in the generation segregation population was compared with the identification results of the above seedling stage artificial inoculation disease resistance, and the EST-SSR marker CSFR33 closely linked with the cucumber brown spot disease resistance gene and the DNA fragment of the brown spot disease resistance gene (SEQ ID No. 1) and the brown spot gene DNA fragment (shown in SEQ ID No.2).

Embodiment 2

[0038] Cucumber germplasm resources XL6-3, W17-2-1, A13, P53, P54, P60-1, P59-1, 66, F51, Q12, Q21, etc. preserved in the breeding room of Tianjin Kerun Cucumber Research Institute were used. The disease resistance of the above materials was identified by artificial inoculation at the seedling stage to obtain disease-resistant materials and disease-susceptible materials. According to the EST-SSR specific primer CSFR33 closely linked with the anti-brown spot gene obtained in Example 1, the genomic DNA of the above-mentioned cucumber material is subjected to PCR amplification, and any material with a specific fragment of 203bp in the amplified product is anti-brown spot Resources; any material with a 207bp specific fragment in the amplified product is a brown spot disease resource; any material that amplifies the two specific fragments at the same time is an impure material. Brown spot resistance resources, or carriers of the brown spot resistance gene, can be used as materials ...

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Abstract

The invention discloses Corynespora cassiicola resistant and suspectible gene fragments and application thereof. The Corynespora cassiicola resistant gene fragment is a nucleotide sequence of SEQ ID No.1; and the Corynespora cassiicola suspectible gene fragment is a nucleotide sequence of SEQ ID No.2. The germ plasm resource of the Corynespora cassiicola resistant gene can be screened by the Corynespora cassiicola resistant gene fragment and the Corynespora cassiicola suspectible gene fragment. Resource resistance screening in cucumber cultivation is of great practical significance. With the sequences as basis, marker-assisted selection and breeding of Corynespora cassiicola resistance can be carried out, and the gene fragments can be used as probes of inspecting Corynespora cassiicola resistant gene in cucumber breeding; the gene fragments also can be used for positioning, constructing and cloning the Corynespora cassiicola, and establishes foundation for improving disease resistanceof cucumber varieties through gene engineering.

Description

technical field [0001] The invention belongs to the technical field of biogenetic engineering, and relates to methods and techniques for conventional hybrid breeding of cucumbers and molecular marker assisted breeding, in particular to gene fragments for resistance to brown spot disease of cucumbers, gene fragments for susceptibility to brown spot diseases of cucumbers and applications thereof. Background technique [0002] Cucumber brown spot disease (also known as target spot disease) [Corynespora cassiicola (Berk. & Curt.) Wei.] is a worldwide distribution disease, which was first reported in Europe in 1906. At present, it has become one of the important diseases that harm the cultivation of cucumbers in open field and protected areas, especially in protected areas such as overwintering greenhouses, winter-spring greenhouses, and spring greenhouses. The host range of the fungus is very wide. In addition to damaging cucumbers, it can infect more than 20 kinds of vegetables...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12Q1/68
Inventor 王惠哲李淑菊杨瑞环管炜
Owner TIANJIN RES INST OF VEGETABLE
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