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Resistance identification method of cucumber corynespora leaf spot disease

A technology for identification of Corynebacterium leaf spot and resistance, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve problems such as identification of resistance to Corynebacterium leaf spot of cucumber without a system, and achieve rapid identification , High repeatability, small effect of environmental factors

Inactive Publication Date: 2015-12-02
텐진인스티튜트오브플랜트프로텍션
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Problems solved by technology

At present, only Lu Haining, Wu Limin, etc. have reported on the effects of the toxin on the physiological indicators of cucumber resistant and susceptible varieties, cell wall damage and changes in the activity of related resistant enzymes, but no systematic application of it to Corynespora cucumber Method for identification of disease resistance of leaf spot disease

Method used

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  • Resistance identification method of cucumber corynespora leaf spot disease

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Embodiment 1

[0024] Example 1, Identification of Resistance of Different Commercial Cucumber Varieties to Corynesporium Leaf Spot of Cucumber

[0025] The identification material of the cucumber resistant to Corynesporium leaf spot of selection in the invention embodiment is: No. Jin You 3, No. Jin You 38 are commercial cucumber varieties, purchased from Tianjin Kerun Cucumber Research Institute; Zhongnong No. 5, Zhongnong No. 8 are commercial cucumber varieties. Cucumber varieties were purchased from the Vegetable and Flower Research Institute of the Chinese Academy of Agricultural Sciences.

[0026] The resistance identification method is as follows:

[0027] ①Preparation of materials for identification of infestation: Sow the four cucumber varieties tested in a greenhouse nutrient bowl (diameter of nutrient bowl is 12cm, matrix composition is peat: vermiculite = 1:2). When the cucumber seedling grows to the period of 3 true leaves, get the second true leaf of each plant as identificati...

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Abstract

The invention relates to a resistance identification method of the cucumber corynespora leaf spot disease. The method includes the steps that a fresh, healthy and complete cucumber true leaf serves as an infection identification material; an improved Czapek liquid culture medium is used for culturing corynespora cassiicola, and primary separation and extraction are performed on culture filtrate to obtain crude toxin filtrate of the corynespora cassiicola; the back of the cucumber detached leaf is inoculated with the crude toxin filtrate in a non-destructive hanging drop mode; then, moisturizing culture is performed in an illumination culture box; the leaf area infected by the toxin filtrate is inspected through a crossing method after the third day, and then the resistance of the cucumber variety to the corynespora leaf spot disease caused by the corynespora cassiicola is obtained. Through the method, the resistance of different varieties of cucumbers to the corynespora cassiicola can be rapidly and accurately identified, and the identification period is greatly shortened. The method is easy to implement, high in repeatability and applicable to resistance identification of the cucumber corynespora leaf spot disease.

Description

technical field [0001] The invention specifically relates to an identification method for in vitro resistant materials of cucumber corynesporium leaf spot, and belongs to the technical field of plant protection. Background technique [0002] by Corynespora polymata ( Corynesporacassiicola ) caused by Corynespora leaf spot (Corynespora leaf afspot) is a serious disease in cucumber production in recent years, the disease mainly infects the leaves, stems and fruits of cucumber. Corynesporium leaf spot of cucumber was first reported in Europe in 1906, and the disease was reported in North Carolina, USA in 1957. In 1960, Qi Peikun and others in my country reported the parasitism of the fungus on cucumbers in my country, and then began to spread in China in 2005. Many cucumber cultivation areas broke out, bringing huge losses to farmers' production and becoming the main disease in cucumber production. According to incomplete statistics, the economic losses caused to farmers exce...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 高苇王勇张春祥刘晓琳霍建飞郝永娟刘春艳
Owner 텐진인스티튜트오브플랜트프로텍션
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