Saline-alkaline tolerant Trichoderma strain, separation and screening method, culture method, application and use method thereof

Abstract

The invention relates to the technical field of microorganisms, and concretely relates to a Trichoderma strain which is separated from an extreme drought desert region, a separation and screening method, a culture method, an application, and a use method thereof. The strain is Trichoderma harzianum M-17, and is preserved in the China Center for Type Culture Collection, and a preservation number isCCTCC NO: M 2018538. The culture method carrys out dark culture of Trichoderma harzianum M-17 on a potato dextrose agar medium at 25-28 DEG C for at least 72 h. The invention relates to application of the saline-alkaline tolerant Trichoderma strain for promoting plant seed germination, seedling growth or/and root growth. The use method irrigates the plant with a bacterial suspension of the saline-alkaline tolerant Trichoderma strain. The method solves the problem of the Trichoderma has growth promoting effect under no salt-alkali stress environment in the prior art, and provides the saline-alkaline tolerant growth-promoting Trichoderma, which can not only enhance the crop stress resistance, but also promotes the growth of crops in the saline-alkaline environment.

Description

Technical field [0001] The invention relates to the technical field of microorganisms, in particular to a salt-tolerant Trichoderma strain, an isolation and screening method, a culture method, an application and a use method. Background technique [0002] For a long time, extensive and in-depth research has been carried out on the improvement and utilization of saline-alkaline soil at home and abroad, and a lot of manpower and material resources have been invested. In the 1980s, most foreign countries focused on the application of chemical modifiers and the destruction of alkalinized layers by deep ploughing. Our country has adopted methods such as changing rice to alkali, applying organic fertilizer, planting green manure, and leveling the land. According to the nature of the improvement measures, the treatment measures for salinized land can be roughly divided into three categories: (1) physical restoration methods; (2) chemical restoration methods; (3) biological restoration ...

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies in the prior art, to provide a saline-alkali-resistant Trichoderma strain, isolation and screening method, culture method, application and use method, which solves the problem that the prior art does not have a solution

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