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Biological control endophytic fungi - Aspergillus restrictus

A technology of Aspergillus and microbial strains, which is applied in the field of microorganisms and can solve the problems of unsafe microbial source pesticide types and other problems

Inactive Publication Date: 2012-09-05
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a kind of endophytic fungus-limited Aspergillus restrictus (Aspergillus restrictus) that isolates and screens out from garlic; Another purpose of the present invention is to provide Method for preventing and treating plant fungal diseases by using the metabolites of the strain

Method used

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  • Biological control endophytic fungi - Aspergillus restrictus
  • Biological control endophytic fungi - Aspergillus restrictus
  • Biological control endophytic fungi - Aspergillus restrictus

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: (Isolation and Screening of Aspergillus restrictus 28)

[0020] The present invention collected garlic in a vegetable garden in the suburbs of Hangzhou, Zhejiang in April 2009, and obtained Aspergillus limitiosa from the steps of surface disinfection, separation of endophytic fungi, cultivation, fermentation, activity determination, and screening of effective strains for inhibiting plant fungal disease pathogens. (Aspergillus restrictus) 28, preserved.

[0021] Determination of the antibacterial activity of Aspergillus restrictus 28: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and mix it with 9 mL of PDA medium cooled to 50 °C quickly, and after cooling, put it in each Place a 4mm-diameter cake of test bacteria such as cucumber solani blight (Rhizoctonia solani) or rice sheath blight (Thanatephorus cucumeris) on the medium plane, and the cake is connected to the center of the petri dish (the diameter ...

Embodiment 2

[0024] Embodiment 2: (preparation method of aspergillus limitus 28 fermentation metabolites)

[0025] Follow these steps:

[0026] (1) Activation culture of strains: move the separated and preserved Aspergillus limitiosa 28 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C±1°C until the hyphae cover the entire petri dish. The hole is punched to obtain the bacteria cake for inoculation;

[0027] (2) Fermentation culture: The fermentation medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH per 1000mL. 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 7 days;

[0028] (3) Extraction of fermented product: After the fermentation ...

Embodiment 3

[0030] Embodiment 3: (inhibitory effect of aspergillus limitae 28 metabolites on rice sheath blight and cucumber solanum blight)

[0031] (1) Accurately weigh 30 mg of the metabolite of Aspergillus limitiosa 28, add 10 mL of sterile water, dissolve it fully by ultrasonic vibration, and finally prepare a mother solution with a concentration of 3000 mg / L;

[0032] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with concentrations of 1250mg / L, 1000mg / L and 500mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and mix with 9mL of PDA cooled to 50°C The culture medium was quickly mixed (the final concentration of the metabolites of Aspergillus limitus 28 was 125mg / L, 100mg / L and 50mg / L), and after cooling, put a 4mm-diameter rice sheath blight fungus or cucumber on each medium plane. Bacterial cake of solanum blight, the cake is connected to the center of the petri dish (the diameter of the petr...

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Abstract

The invention relates to the microbial technology field. The garlic endophytic fungi are obtained from a garlic living body with endophytic fungi microbe separation technology, wherein the garlic living body is obtained at a vegetable garden in the suburb of Hangzhou city Zhejiang province. Through the microbe taxology identification, the garlic endophytic fungi are named as Aspergillus restrictus 28. The bacterial strain is preserved at China General Microbiological Culture Collection Center with a preservation date December 31st 2010 and a preservation registry number CGMCC No. 4507. The most substantial characteristic of the endophytic fungi is that through liquid fermentation the bacterial strain can produce an active metabolite which has an inhibition effect on pathogen of plant fungous disease such as Rhizoctonia solani, Thanatephorus cucumeris and the like. The bacterial strain is an important microbial resource.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to the cultivation of Aspergillus restrictus and the application of its metabolites for biocontrol endophytic fungi. Background technique [0002] There are a large number of endophytic fungi in plants. Endophytic fungi were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytic fungi can enhance the host's disease resistance, improve plant productivity, stress resistance and insect resistance, etc. These functions are due to the reproduction and growth of endophytic fungi in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytic fungi may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant endop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01P3/00C12R1/66
Inventor 申屠旭萍俞晓平董胜张郝培应边亚琳马正
Owner CHINA JILIANG UNIV
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