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Use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum niveum

A technology of watermelon wilt pathogen and Streptomyces shioya, which is applied in the field of microorganisms and can solve problems such as insecurity

Inactive Publication Date: 2015-03-04
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide an endophytic actinomycete isolated and screened out from Chinese wolfberry for the deficiency that chemical pesticides are unsafe and the types of microbial source pesticides; Streptomyces sioyaensis (Streptomyces sioyaensis); Another object is to provide a method for utilizing the strain metabolites to prevent and treat plant fungal diseases

Method used

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  • Use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum niveum
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum niveum
  • Use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum niveum

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Embodiment 1

[0018] Embodiment 1: (isolation and screening of Streptomyces shioya 107)

[0019] The present invention collected Chinese wolfberry stems in Hangzhou, Zhejiang in July 2012, and obtained Streptomyces shiyaya from them through the steps of surface disinfection, endophyte isolation, cultivation, fermentation, activity determination, and screening of active strains of pathogenic bacteria that inhibit plant fungal diseases. 107. Save.

[0020] Determination of antibacterial activity of Streptomyces shioya 107: Take 1mL of the fermentation supernatant filtered through a 0.22μm membrane in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and place on each medium plane after cooling Put 1 rice sheath blight (Thanatephorus cucumeris) or cucumber wilt (Fusarium oxysporum.sp.cucumebrium Owen) or cucumber blight (Rhizoctonia solani) or watermelon wilt (F.oxysporum f.sp. niveum) or tomato gray mold (Botrytis cinerea) and other test bacteria cakes, the cakes are connec...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Streptomyces shioya 107 fermentation metabolites)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Streptomyces shioya 107 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C±1°C until the inclined surface of the test tube is covered with spores for inoculation;

[0026] (2) Fermentation culture: the fermentation culture liquid contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, 5g of peptone in every 1000mL, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 10g, 60mL of fermentation broth per 300mL Erlenmeyer bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick a spore of Streptomyces platinumensis 107 under sterile conditions and inoculate at 28°C±1°C , the shaker speed is 180 rpm, and the fermentation culture is 128h;

[0027] (3) Extraction of fermented product: After the fermentation is completed, the fermented liqui...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Streptomyces shioya 107 metabolites to rice sheath blight, cucumber solanum blight, tomato Botrytis cinerea, cucumber fusarium wilt and watermelon wilt)

[0030] (1) Accurately weigh 0.20 g of the metabolite of Streptomyces shioya 107, add 10 mL of sterile water, dissolve it fully by ultrasonic vibration, and finally prepare a mother solution with a concentration of 20 g / L;

[0031] (2) Determination of antibacterial activity: Take the above-mentioned mother liquor and dilute it into solutions with a concentration of 2g / L and 1g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C. Evenly (the final concentration of the metabolites of Streptomyces shioya 107 is 200mg / L and 100mg / L), after cooling, put a 4mm diameter of rice sheath blight or cucumber solanum blight or tomato ash on each medium plane Bacteria cakes of mold fungi or cucumber wilt bact...

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Abstract

The invention relates to the field of microbial technology and especially relates to a use of Streptomyces sioyaensis metabolite in prevention and control of Fusarium oxyspirum f. sp. niveum. Llex cornuta endophyte separated from the llex cornuta living bodies in Hangzhou of Zhejiang province by an endophyte microorganism separation technology is identified as Streptomyces sioyaensis by microbial taxonomy and is named as Streptomyces sioyaensis 107. The Streptomyces sioyaensis 107 is preserved in the China general microbiological culture collection center on December 12, 2012 and has a preservation number of CGMCC No. 6984. Through liquid fermentation, the Streptomyces sioyaensis 107 can produce the active metabolites having effects of inhibiting plant fungal disease pathogenic bacteria such as Rhizoctonia solani and Thanatephorus cucumeris and is an important microbe resource in agriculture.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of Streptomyces sioyaensis (Streptomyces sioyaensis) and the application of its metabolites for biocontrol endophytic actinomycetes. Background technique [0002] There are a large number of endophytes in plants. Endophytes were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytes can enhance the host's disease resistance, improve plant productivity, and resist stress and insects. These functions are due to the reproduction and growth of endophytes in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytes may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant endophyte...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01P3/00
Inventor 汤谷申屠旭萍俞晓平刘叶丹陈昳丽俞叶微曾惠娟
Owner CHINA JILIANG UNIV
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