The invention provides a huperzine a lyotropic liquid crystal preparation and a preparation method thereof. The huperzine a lyotropic liquid crystal preparation is prepared from the following components in parts by weight: 0.12 to 0.7 part of huperzine a, 10 to 18 parts of organic solvent, 12 to 70 parts of phospholipid, and 12 to 59.5 parts of grease. According to the huperzine a lyotropic liquid crystal preparation provided by the invention, the phospholipid, the grease and the organic solvent are used as carriers of the huperzine a, so that the solubleness of the huperzine a is effectively improved, the defect that the huperzine a is insoluble in water is overcome, the pharmacological function of the huperzine a is played favorably; meanwhile, under the condition of the limited component proportion, the huperzine a lyotropic liquid crystal preparation encounters water to form a lyotropic liquid crystal after being injected into a human body, so that the adhesion of a medicine and the preparation is improved, the medicine diffusion speed is retarded, the administration period is increased, the administration times are reduced, and thus the compliance of a patient is greatly improved.