36 results about "Pteridophyte" patented technology

A method for cultivating plants on the slope of bare rock body, railway, high way, etc includes such steps as collecting the spores of pteridophyte and the seeds of other plants, treating the spores and seeds, mixing them with matrix, fixing mesh onto said slope, spraying foreign soil and plant seeds, hydraulically spraying the spores of pteridophyte, covering cloth, and nursing.

The invention discloses a method for tissue culture method of huperizia serrata. The method comprises the following steps of: selecting an explant; killing surface bacteria; performing inoculation and cultivation; killing endophyte; performing propagation cultivation; and performing rooting cultivation. The huperizia serrata is a precious medicinal pteridophyte which has a tough requirement on a habitat, grows slowly under a natural condition and has a long production period. In a conventional method, propagation coefficient and biomass are low and requirements on medicament production cannot be met. Due to the adoption of the method, the differentiation rate of a huperizia serrata test tube plantlet is up to 90 percent, monthly multiplication multiple is up to 2.1 to 3.5, rooting rate is up to 100 percent, a root system grows quickly, root number is up to 1.6 to 3.4 per plant, a regeneration seedling grows healthily and the root system is developed. The method has the advantages of effectively restraining the pollution and browning of a huperizia serrata explant, promoting the survival and growth of the explant and providing important technical support for the realization of manual mass planting of the huperizia serrata along with simpleness, convenience, practicability, high efficiency and low cost.

The invention relates to a method for plant restoring soil of arsenic contamination, which is realized through inoculating arbuscular mycorrhizal fungi to centipedefern, a kind of pteridophyte, thus producing fungus root, the method comprises the steps of, (1) collecting raw pteridophyte material, obtaining spores of pteridophytes, cultivating young plant sprout, (2) producing fungus roots from pteridophyte, (3) transplanting the adult strain of young sprouts to arsenic contaminated soil. The invention can be applied to aid the arsenic super rich centipedefern to adapt to arsenic contamination environment.

The invention provides an intensive cultivation method for dendrobium officinale in a greenhouse. The combined cultivation medium used in the cultivation method comprises a medium A and a medium B, wherein the medium A comprises the following ingredients in parts by weight: 20-50 parts of pine bark, 20-50 parts of camphor tree bark, 20-50 parts of palm tree bark and 20-30 parts of pteridophyte roots; the medium B comprises the following ingredients in parts by weight: 10-30 parts of pine park, 20-30 parts of bagasse, 10-20 parts of silkworm excrement, 10-20 parts of corncobs, 20-25 parts of mulberry stems, 10-15 parts of edible fungus sticks, 10-15 parts of coco coir and 10-15 parts of clay; the cultivation method comprises the following steps of building the greenhouse, preparing a seedbed, paving the mediums, hardening seedlings, transplanting the seedlings, field managing, plant disease and pest damage controlling, and harvesting. The survival rate of the dendrobium officinale can reach more than 99% and the occurrence rate of the plant disease and insect pest damage is low by adoption of the technology of the intensive cultivation method.

The invention relates to a plant-derived growth-promoting organic medical fertilizer, and belongs to the technical field of organic fertilizers. The plant-derived growth-promoting organic medical fertilizer comprises the following components in percentage by weight: 10 to 35 percent of pteridophyte rhizome, 10 to 38 percent of pine needle, 10 to 36 percent of leguminous plant rhizome, 2 to 10 percent of neem, 2 to 10 percent of tung oil shell, 10 to 35 percent of chicken manure, 5 to 20 percent of pigeon manure, 0.1 to 1.0 percent of zymophyte and 0.05 to 1.0 percent of beneficial microbial strain. The organic medical fertilizer not only has sufficient fertilizer efficiency for promoting the growth of crops but also can effectively prevent and control pests. The invention also provides a method for preparing the plant-derived growth-promoting organic medical fertilizer.

The invention discloses a huperzia serrate endophytic fungus which is obtained from a pteridophyte, namely, a huperzia serrate plant by adopting a separating and purifying technology, is named Ceriporia lacerate HS-ZJUT-C13A and is collected in the China Center for Type Culture Collection on October 28, 2012 with the collection number CCTCC M 2012433. The strain provided by the invention can be used for preparing a compound, namely, 8alpha, 15alpha-epoxydized huperzine A with a nerve protection effect. A conversion method has the advantages of simple fermentation conditions, high substrate conversion rate and the like, is easily cultures the strain, has the potential of industrial large-scale production, is a novel way for obtaining the compound, protects valuable and rare medicinal huperziaceae plant resources from being damaged and can provide a novel thought for reliving the current situation of lack of huperzine A in clinical administration.

The invention belongs to the technical field of biological engineering, and relates to a culture medium for the spore germination and seedling culturing of pteridophyte, which is characterized by consisting of the following nutritional components: 2 to 60 mmol.L<-1> nitrate ions, 1 to 30 mmol.L<-1> ammonium ions, 0.3 to 8 mmol.L<-1> phosphate radical ions, 2.7 to 66 mmol.L<-1> potassium ions, 0.37 to 12.0 mmol.L<-1> calcium ions, 0.18 to 1.8 mmol.L<-1> magnesium ions, 0.173 to 1.73 mmol.L<-1> sulfate ions, 10 to 100 mumol.L<-1> chelated ferric salt or chelated ferrous salt, 10 to 200 mumol.L<-1> boric acid, 10 to 100 mumol.L<-1> divalent manganese ions, 3 to 30 mumol.L<-1> zinc ions, 0.5 to 8 mumol.L<-1> divalent copper ions or complexes thereof, 0.1 to 1 mumol.L<-1> molybdate ions, 0.01 to 0.1 mumol.L<-1> divalent cobalt ions, 0.05 to 12 mumol.L<-1> chloride ions, 0.3 to 30 mumol.L<-1> thiamine hydrochloride and the balance of water, wherein the pH value of the culture medium is between 4.0 and 9.0. The culture medium can improve the germination rate of spores.

A method for controlling the pollution of As to soil and water features that the pteridophytes able to collect As, such as Pteris oshimensis, P. fauriei, P. aspericaulis and P. multifida, are planted in the soil or water to be controlled for absorbing the As from soil and water, and their plant stems on the ground are periodically harvested.

The invention relates to an organic feed containing a taxol component with an anticancer function, which comprises the following components in percentage by weight: 5-38 percent of Chinese yew, 3-45 percent of pteridophytes and auxiliary materials. The feed not only has no chemically-synthesized addictive, but also combines taxol with the anticancer function with the pteridophytes, so that animals fed by the feed are healthy and strong, have strong activity and can be healthily and safely eaten by normal groups and cancer patients. The feed cannot cause splash, can indirectly and effectively prevent and treat cancer and promote the virtuous circle of ecological environment.

The invention discloses a method for platycerium wallichii tissue culture and rapid propagation and relates to a method for obtaining the platycerium wallichii test-tube plantlet in a short time through a plant tissue culture technology. The in-vitro tissue culture and rapid propagation method of the platycerium wallichii is built by taking the platycerium wallichii tender sporophyl as an explant, disinfecting the explant, and performing GGB induction, proliferating, differentiating and rooting. The growth cycle is shortened, the shortage of the platycerium wallichii commercialized produced seedlings can be relieved and a foundation is laid for innovating the germplasm resources of the pteridophyte in future.

The invention discloses a pteridophyte seedling raising device. The pteridophyte seedling raising device comprises a seedling raising basin, a sowing clapboard, and a culture medium pushout constructional element. The seedling raising basin comprises a basin body (2) and a cover body (3) which is matched with the basin body. The basin body is composed of a bottom wall and side walls. Through-holes are formed in the bottom wall. The cover body is composed of a top wall and side walls. Convection holes are formed in the side wall of the cover body. The sowing clapboard (4) is arranged in the basin body and is composed of clapboard side walls and side walls of griddings arranged inside the clapboard side walls. The culture medium pushout constructional element (5) comprises a base seat and a convex circular cylinder which is arranged on the base seat and matched with the shape of gridding cavities. The pteridophyte seedling raising device can improve efficiency of seedling and transplanting, reduce cross infection probability between sporophytes, reduce damage of gametophytes and sporophytes in transplanting process, and has the advantages that cultivating room is made full use of, stability of development environment of spores and seeding transplanting process standardization are guaranteed, management cost is reduced and the pteridophyte seedling raising device is suitable for pteridophyte seedling standardization and large-scale production.

The present invention relates to a pteridophyte propagation method in limestone district, belonging to the field of flowers, plants and Chinese medicinal herbs cultivation technology. Said method includes the following steps: collecting spores in spore ripening season of pteridophyte, placing the collected spores into a clean container, placing said container in a ventilating shade place and making it be naturally dried; in 2-3 month of spring sowing the spores into the moss cluster grown on the nightside limestone, after the sporophyte is grown for 20-30 days, transplanting the sporophyte together with moss cluster into green-house or flowers nursery garden to make cultivation.

The invention relates to a pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method, and belongs to the technical field of a plant cultivation method. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method is characterized in that a green spheroid generated by inducing a pteridophyte aseptic seedling under the condition of isolated culture is used as a material, and <60>Cor rays are used as radiation sources; the green spheroid subjected to irradiation treatment is transferred to a grow-up seedling culture medium containing 0.2mg / L of additional indolebutyric acid (IBA) and germinates, thus a complete plantlet with roots, stems and leaves is formed; the dosage rate of the<60>Cor rays is 1Gy / min, the irradiation dose is 80Gy, and the seedling rate of the green spheroid of neocheiropteris palmatopedata is 38%; the seedling rate of the green spheroid of pteris multifida poir is 26%. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method provided by the invention provides a new technology for development of irradiation mutagenesis breeding of pteridophyte on the organ level and is suitable for breeding of pteridophyte new varieties.

The invention discloses a huperzia serrata endophytic fungus which is obtained by separation from a stem part of a living body of a pteridophyte huperzia serrata plant and classified and named as peyronellaea glomerata HS-ZJUT-XW12, wherein the strain is collected in China Center for Type Culture Collection (CCTCC) on the collection date of October 28, 2012 with the collection number of CCTCC NO: M 2012434. The strain provided by the invention is found by inventors after years of creative experimental labor, and the strain is firstly used for fermentation and culture so as to prepare a compound FPBA with liver-protecting activity; the preparation method has the advantages of simple fermentation conditions, easiness in culture of the strain, few process steps, short production period and the like, and further has potential for realizing industrial large-scale production; a new way is provided for seeking sources for developing pyrrole type liver-protecting medicines, and the shortage of clinical medicines can be alleviated to a certain extent.

The invention provides a sowing method suitable for pteridophytes in a slope restoration process. The sowing method of the pteridophytes comprises the steps as follows: step 1, immersing a polyurethane substrate in a spore suspension of pteridophytes to obtain a polyurethane substrate attached with spores; step 2, performing sowing. According to the method, the spores are adsorbed onto the polyurethane substrate, the polyurethane substrate plays two roles in the germination process of the pteridophytes: on one hand, the polyurethane substrate can provide a growth carrier for the spores and prevent the condition that the mitoplast at the initial stage of germination of spores cannot grow into young gametophytes easily due to coverage of spores with soil or other types of substrates in the sowing process, on the other hand, the good porosity structure of the polyurethane substrate can provide specific microenvironments for growth of sporophytes at the early stage, guarantee air, moisture, nutrients and the like required by growth of the sporophytes and greatly increase the survival rate of the spores.

The invention provides a method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata, belonging to the technical field of plant culture methods. The existing technology for regenerating plants by inducing adventitious buds with pteridophytes is still on the laboratory research stage and is limited to rhynioceae and nephrolepidaceae. The method is characterized in that the leaves of davalliaceae davallia bullata aseptic seedlings are taken as the explant materials; the adventitious buds are induced and generated on the culture media to which 2.0mg / L of phenylurea cytokinins (CPPU) and 0.1mg / L of indolebutyric acid (IBA) are added; the induction frequency of the adventitious buds reaches 95%; multiplication of the adventitious buds is carried on the culture media to which 0.5mg / L of CPPU and 0.1mg / L of IBA are added; the multiplication coefficient of each generation of the adventitious buds is 5; and the seedling rate of the adventitious buds on the culture media to which 0.2mg / L of IBA is added reaches 100% and the adventitious buds grow to become complete plantlets with roots, stems and leaves. The method is suitable for propagating plantlets of davallia bullata and provides conditions for developing screening of cell mutants and gene transformation study.

The invention provides a traditional Chinese medicine pesticide for preventing and controlling peach diseases, as well as a preparation method and application thereof. The pesticide is prepared from 50-70 parts of wormwood plant, 30-50 parts of pteridophyte, 20-30 parts of azedarach, 10-30 parts of Chinese chives and 10-30 parts of 90% ethyl alcohol. The traditional Chinese medicine pesticide is effective in preventing and controlling peach diseases, the medicinal material sources are abundant and are easily acquired, the cost is low, the application dosage of the pesticide is reduced and the traditional Chinese medicine pesticide is suitable for popularization and application in fruit farmers.

The invention discloses an artificial insemination method for Filicinae, and belongs to the technical field of plant artificial insemination. In order to solve the problem of low seedling rate of pteridophytes, the invention provides an artificial insemination method of Filicinae, which comprises the following steps: inoculating spores into a culture medium, obtaining female gametophytes and malegametophytes of the Filicinae by using a conventional spore culture method, after a sexual organ is mature, sucking mature sperms and a prepared induction liquid, sequentially dropwise adding the mature sperms and the prepared induction liquid to a mature archegonium of the female gametophyte, and enabling the sperms to enter the archegonium to be combined with ova under the guidance of the induction liquid, so as to complete artificial insemination. According to the sperm taking and artificial directional insemination technology provided by the invention, the fertilization rate of the gametophyte can reach 30%-50% and is 3-5 times that under conventional culture conditions, the phenomenon that a single gametophyte has two embryonic developments often occurs, and the hybridization technology of pteridophytes is simpler, more convenient and easier to implement.

The invention provides a dendrobium officinale planting method under a bamboo forest. The dendrobium officinale is planted under the nature environment bamboo forest, and special fertilizer of dendrobium officinale is prepared from wormwood, pteridophyte, cow dung, herb residues, loess, moss, naphthylacetic acid, and naphthylacetic acid; nutrition matters of every component are coordinated and matched, and enriched in various nutrients for the full growth process of the dendrobium officinale; the method for planting the dendrobium officinale can improve the disease resistance of the dendrobium officinale; the chemical pesticide is not required to spray basically during the whole planting cycle, thus the quality of the dendrobium officinale product is improved, the yield is promoted, and the planting economic benefit is increased.

The invention discloses a sterilization and inoculation method of pteridophyte spores. One set of sterilization and inoculation method applicable to trace pteridophyte spores is designed by combining a sterilization method of a tissue culture technology with tools including a DNA (Deoxyribonucleic Acid) purification column and the like, and is applicable to an in-vitro preservation process. According to the technology disclosed by the invention, the problems of insufficient sterilization and interbreeding and disinfection liquid residues, which easily occur in a disinfection process, are solved; the pollution rate can be effectively reduced and the inoculation efficiency is improved. The sterilization and inoculation method disclosed by the invention has the advantages that the trace pteridophyte spores can be efficiently disinfected and inoculated; experimental apparatuses are easy to obtain; the cost is low, the operation is convenient, the inoculation efficiency is high and the like.

The invention belongs to the technical field of pteridophyte spore propagation, and discloses a rapid propagation method of pteridophyte spores. The method comprises the steps of S1, collecting mature spores of a pteridophyte, and removing impurities; S2, preparing MS macroelements, gibberellin, carbendazim and sodium alginate into a gel liquid, and then stirring the gel liquid with the spores evenly to prepare a spore gel liquid; S3, extracting the spore gel liquid, dropwise adding the spore gel liquid into a CaCl2 solution for curing reaction to form spore balls, and taking out and cleaning the spore balls; S4, using peat soil and perlite as a matrix, watering the matrix sufficiently one day before sowing, and a tray is stacked at the bottom for water retention; S5, sowing the spore ball on the surface of the matrix of a plug, wherein one ball is sowed in every plug, there is no need to cover the soil, and a moisture retention cover is covered; S6, maintenance management. According to the rapid propagation method of the pteridophyte spores, fine spores are prepared into artificial seeds, by covering nutrient substances and a bacteriostatic agent, the growth of the spores is accelerated, and thus the spores are grown into mature gametophytes in a short period; meanwhile, the spore density is effectively controlled, transplanting is not needed, and the spores is directly cultivated into seedlings.

The invention discloses a medicament for treating and preventing malignant tumors and a preparation method thereof. The medicament comprises, by weight, 25 to 35 parts of Poria cocos, 15 to 20 parts of radix paeoniae alba, 3 to 6 parts of honeysuckle, 4 to 8 parts of gynostemma pentaphylla, 10 to 15 parts of Chinese actinidia roots, 15 to 20 parts of black nightshade, 12 to 14 parts of alexipharmic pteridophyte, 2 to 5 parts of Chinese angelica, 2 to 4 parts of turtle shells, 5 to 8 parts of pseudo-ginseng, 2 to 4 parts of snow lotus herb, and 2 to 5 parts of smilax bockii warb. Besides, the preparation method comprises the following steps of crushing, extracting, concentrating, and preparing of an extract into a specific dosage form.

The invention belongs to the technical field of weathered crust rare earth enrichment, and particularly relates to a method for enriching rare earth elements in a weathered crust by using pteridophytes. The method comprises the following steps of 1) soil pretreatment; and 2) pteridophyte planting, wherein the pteridophytes are planted on the weathered crust, and the pteridophytes comprise one or more of blechnum orientale, dicranopteris pedata, athyrium filix-femina, dryopteris filix-mas, asplenium trichomanes, diplopterygium glaucum, lindsaea odorata, woodwardia japonica, stenoloma chusana, adiantum flabellulatum, cibotium barometz, microlepia strigosa and diplazium donianum. Different pteridophytes are planted on different structural layers of the weathered crust in different modes to be used for adsorbing rare earth elements in soil, so that the rare earth resources can be effectively utilized; and the effect of improving the surrounding ecological environment can be achieved by planting the pteridophytes in the weathered crust soil. Compared with an existing rare earth mining technology, the method cannot cause secondary pollution. The method provides an economical and effective solution way for realizing ecological management of rare earth polluted land and rare earth reclamation.

The invention discloses a planting box for planting deep sea pteridophyte indoors. The planting box comprises a base. The planting box is characterized in that the base is located on the ground, a deep sea pteridophyte planting mechanism is arranged on the base, a deep sea pteridophyte ecological supply mechanism is arranged below the deep sea pteridophyte planting mechanism, a light insulating mechanism is arranged outside the deep sea pteridophyte planting mechanism, a controller and a battery box are arranged above the base, the power input end of the controller is electrically connected with the battery box, the signal input end of the controller is electrically connected with a carbon dioxide concentration sensor, an oxygen concentration sensor and a water pressure sensor, and the signal output end of the controller is electrically connected with the water pressure sensor, a new water supply electromagnetic valve, a deep sea pteridophyte planting box water pressure holding pump, anew water supply electromagnetic valve and an old water drainage electromagnetic valve. The planting box has the advantages of being simple in structure and high in practicality.

The invention relates to a pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method, and belongs to the technical field of a plant cultivation method. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method is characterized in that a green spheroid generated by inducing a pteridophyte aseptic seedling under the condition of isolated culture is used as a material, and <60>Cor rays are used as radiation sources; the green spheroid subjected to irradiation treatment is transferred to a grow-up seedling culture medium containing 0.2mg / L of additional indolebutyric acid (IBA) and germinates, thus a complete plantlet with roots, stems and leaves is formed; the dosage rate of the<60>Cor rays is 1Gy / min, the irradiation dose is 80Gy, and the seedling rate of the green spheroid of neocheiropteris palmatopedata is 38%; the seedling rate of the green spheroid of pteris multifida poir is 26%. The pteridophyte green spheroid <60>Cor ray irradiation mutagenesis method provided by the invention provides a new technology for development of irradiation mutagenesis breeding of pteridophyte on the organ level and is suitable for breeding of pteridophyte new varieties.

The invention provides an earthworm culturing matrix and a preparation method thereof. According to a designed and screened formula, the earthworm culturing matrix is prepared from 400 to 600 parts of soil, 100 to 300 parts of paper pulp sludge, 100 to 200 parts of wormwood plant, 100 to 200 parts of pteridophyte, 100 to 200 parts of straw scraps and 50 to 100 parts of dried cow dung. The earthworm culturing matrix is reasonable in formula, and is abundant in nutrients; a reasonable carbon nitrogen ratio is provided for a fermenting process, so that the earthworm culturing matrix has higher superior quality, the quality of the earthworm culturing matrix is improved effectively, the toxic and harmful substances in the earthworm culturing matrix are lowered, yield is increased, and the quality is improved.

The invention discloses a pteridophyte seedling raising device. The pteridophyte seedling raising device comprises a seedling raising basin, a sowing clapboard, and a culture medium pushout constructional element. The seedling raising basin comprises a basin body (2) and a cover body (3) which is matched with the basin body. The basin body is composed of a bottom wall and side walls. Through-holes are formed in the bottom wall. The cover body is composed of a top wall and side walls. Convection holes are formed in the side wall of the cover body. The sowing clapboard (4) is arranged in the basin body and is composed of clapboard side walls and side walls of griddings arranged inside the clapboard side walls. The culture medium pushout constructional element (5) comprises a base seat and a convex circular cylinder which is arranged on the base seat and matched with the shape of gridding cavities. The pteridophyte seedling raising device can improve efficiency of seedling and transplanting, reduce cross infection probability between sporophytes, reduce damage of gametophytes and sporophytes in transplanting process, and has the advantages that cultivating room is made full use of, stability of development environment of spores and seeding transplanting process standardization are guaranteed, management cost is reduced and the pteridophyte seedling raising device is suitable for pteridophyte seedling standardization and large-scale production.