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Method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata

A high-frequency technology of Pennisetum fern, applied in the field of plant cultivation, high-frequency plant regeneration of Pennisetum leaf induced adventitious buds

Inactive Publication Date: 2011-08-03
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Up to now, among the 12,000 species of ferns, only a very small part have successfully undergone tissue culture. , the problem of low multiplication rate (Han Jing, Zhao Li. Research progress on fern reproduction [J]. Anhui Agricultural Sciences, 2005, 33(7): 1261~1263)

Method used

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  • Method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata
  • Method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata
  • Method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata

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Experimental program
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Embodiment Construction

[0030] 1. Materials and methods

[0031] 1.1 Test material

[0032] In the order Pteridophyta ( Polypodiales ) Drynariae ( Davalliaceae ) of Pennisetum ( Davallia bullata ) aseptic seedlings (Pennensis fern seedlings were purchased from Nanjing Wanbo Flower Market, and the aseptic seedlings were cultivated by our laboratory) as explants.

[0033] culture medium

[0034] The basic medium consists of MS macroelements, MS trace elements (Murashige T, Skoog F. A revised medium from rapid growth and bioassays with tobacco tissue cultures[J]. Physiol plant, 1962, 15:473~497 ) and B 5 Vitamin (Gamborg O L, Miller R A, Ojima K. Nutrient requirements of suspension cultures of soybean root cells[J]. Exp. Cell Res., 1968. 50:151~158), a commonly used medium in plant tissue culture , sucrose 30 g / L, agar stick (Chengfeng brand, Quangang Chemical Factory, Quanzhou City, Fujian Province) 8 g / L;

[0035] Adventitious bud induction medium: add 2.0 mg / L 6-benzylaminopurine (abbr...

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Abstract

The invention provides a method for regenerating plants under high frequency by inducing adventitious buds with leaves of davallia bullata, belonging to the technical field of plant culture methods. The existing technology for regenerating plants by inducing adventitious buds with pteridophytes is still on the laboratory research stage and is limited to rhynioceae and nephrolepidaceae. The method is characterized in that the leaves of davalliaceae davallia bullata aseptic seedlings are taken as the explant materials; the adventitious buds are induced and generated on the culture media to which 2.0mg / L of phenylurea cytokinins (CPPU) and 0.1mg / L of indolebutyric acid (IBA) are added; the induction frequency of the adventitious buds reaches 95%; multiplication of the adventitious buds is carried on the culture media to which 0.5mg / L of CPPU and 0.1mg / L of IBA are added; the multiplication coefficient of each generation of the adventitious buds is 5; and the seedling rate of the adventitious buds on the culture media to which 0.2mg / L of IBA is added reaches 100% and the adventitious buds grow to become complete plantlets with roots, stems and leaves. The method is suitable for propagating plantlets of davallia bullata and provides conditions for developing screening of cell mutants and gene transformation study.

Description

technical field [0001] The invention relates to a high-frequency plant regeneration method for inducing adventitious buds from leaves of Pennisetum fern, which relates to a plant regeneration and test-tube seedling micropropagation technology suitable for in vitro culture of fern somatic cells, and belongs to the technical field of plant culture methods. Background technique [0002] Up to now, among the 12,000 species of ferns, only a small part have successfully undergone tissue culture. Although some genera and species have been successfully tissue cultured, they have the problems of long cycle and low proliferation rate (Han Jing, Zhao Li. Fern Research progress on plant-like reproduction [J]. Anhui Agricultural Sciences, 2005, 33(7): 1261~1263). Compared with spores as explants, the use of sporophyte rhizome tips, young leaves, etc. as explants for tissue culture has the advantages of fast seedling growth and the like. Therefore, the study of fern tissue culture with ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 叶晓青佘建明邓衍明汤日圣童红玉
Owner JIANGSU ACAD OF AGRI SCI
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