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Novel method for extracting huperzine A from huperzia serrata

A technology of Huperzine A and a new method, applied in the field of medicine, can solve the problems of damage to instruments and equipment, long production cycle and high production cost, and achieve the effects of less damage to instruments and equipment, low cost and low toxic and side effects

Inactive Publication Date: 2013-01-09
GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to develop a new method for extracting huperzine A from the new resource Huperzia longifolia (a variant of Huperzia serrata) aiming at the serious damage to instruments and equipment in the background technology, long production cycle and high production cost.

Method used

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  • Novel method for extracting huperzine A from huperzia serrata
  • Novel method for extracting huperzine A from huperzia serrata
  • Novel method for extracting huperzine A from huperzia serrata

Examples

Experimental program
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Effect test

Embodiment 1

[0056] (1). Put 10Kg of crushed Huperzia longifolia medicinal material into the extraction tank, add 20 times the amount of 20% ethanol water, and reflux extraction at 75°C for 3 times, the first time is 5 hours, and the second time is 4 hours , for the third 4 hours, combine the extracts for 3 times;

[0057] (2). The above extract was concentrated under reduced pressure at 50°C to 10% of the original volume. The concentrated solution was adjusted to pH 2-3 with 5‰ HCl, extracted three times with ethyl acetate equal to the volume of the concentrated solution, and acetic acid The ethyl ester was discarded, and the acidic aqueous layer was set aside;

[0058] (3). Adjust the pH of the acidic water layer to 9-10 with 1:1 (v / v) ammonia water, extract 4 times with chloroform equal to the volume of the concentrated solution, combine the chloroform extracts and concentrate under reduced pressure to obtain the total alkaloids;

[0059] (4). The total alkaloids are eluted by MPLC (RP...

Embodiment 2

[0062] (1). Put 30Kg of the crushed Huperzia longifolia medicinal material into the extraction tank, add 15 times the amount of 40% ethanol water, and reflux extraction at 75°C for 4 times, the first time is 5 hours, and the second time is 4 hours , 4 hours for the third time, 3 hours for the fourth time, and combine the extracts for 4 times;

[0063] (2). The above extract was concentrated under reduced pressure at 50°C to 10% of the original volume, and the concentrated solution was adjusted to pH 2-3 with 5‰ HCl, extracted 4 times with ethyl acetate equal to the volume of the concentrated solution, acetic acid The ethyl ester was discarded, and the acidic aqueous layer was set aside;

[0064] (3). Adjust the pH of the acidic water layer to 9-10 with ammonia water, extract 4 times with chloroform equal to the volume of the concentrated solution, combine the chloroform extracts and concentrate under reduced pressure to obtain the total alkaloids;

[0065] (4). The total alka...

Embodiment 3

[0068] (1). Put 50Kg of the crushed Huperzia longifolia into the extraction tank, add 10 times the amount of 60% ethanol water, and reflux extraction at 75°C for 3 times, the first time is 5 hours, and the second time is 4 hours , for the third 4 hours, combine the extracts for 3 times;

[0069] (2). The above extract was concentrated under reduced pressure at 50°C to 10% of the original volume, and the concentrated solution was adjusted to pH 2-3 with 5‰ HCl, extracted 4 times with ethyl acetate equal to the volume of the concentrated solution, acetic acid The ethyl ester was discarded, and the acidic aqueous layer was set aside;

[0070] (3) Adjust the pH of the acidic water layer to 9-10 with ammonia water, extract 5 times with chloroform equal to the volume of the concentrate, combine the chloroform extracts and concentrate under reduced pressure to obtain the total alkaloids;

[0071] (4). The total alkaloids are eluted by MPLC (RP-18) method, using methanol water as the...

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Abstract

The invention relates to a novel method for extracting huperzine A from Huperziaserrataf.longipetiolata (Spring) Ching, and relates to the technical field of medicines. The novel method comprises the technical scheme as follows: (1) preparing extracting liquid; (2) purifying the extracting liquid; (3) preparing the total alkaloid of huperzia serrata; (4) preparing a rough product of huperzine A; and (5) preparing high-purity huperzine A, thus obtaining the white solid huperzine A with the purity of 98% to 99%. The novel method of extracting the huperzine A provided by the invention has the advantages that: (1) the new resource huperzia serrata is served as the raw material; and the single source of the medicine and technology method for extracting huperzine A from huperziaserrataf.longipetiolata (Spring) Ching are expanded; (2) 20% to 60% of ethanol water is adopted for extracting, therefore, little damage is brought to instruments and equipment, and low toxic and side effects are generated; and (3) the huperzine A is enriched through a reverse phase (Middle Pressure Liquid Chromatography) is adopted, thus, the cost is low, the purity is high, the yield is high, and the mass industrial production can be easily carried out.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a preparation method for firstly separating the alkaloid huperzine A from Huperzine longhandle produced in Liuzhi area of ​​Guizhou province. Huperzine A easily penetrates the blood-brain barrier, has a strong inhibitory effect on acetylcholinesterase (AchE) in the brain, significantly increases the level of acetylcholine in the brain, promotes learning and memory processes, or significantly improves memory impairment. Background technique [0002] Long handle Huperus Huperzia serrata f. longipetiolata (Spring) Ching is a variant of the Huperzia serrata fern in the Huperdaceae family. Distributed in Guizhou, Sichuan, Chongqing, Yunnan and other places in Southwest my country on hillsides, river valleys, streamsides, under forests, forest margins or under bushes at an altitude of 300-1900 meters. It has the effects of dispelling blood stasis, reducing swelling, stopping bleedin...

Claims

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Application Information

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IPC IPC(8): C07D221/22
Inventor 张敬杰赵勤实吴兴德刘亚华李齐激潘炉台
Owner GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE
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