Bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B and usage

Abstract

The invention provides a bacillus amyloliquefaciens SXZ-N2 strain producing huperzine A and huperzine B. The strain can produce huperzine A and huperzine B. The strain is named as SXZ-N2 and belongs to bacillus amyloliquefaciens. The strain is collected in China Center for Type Culture Collection (CCTCC) on September 14, 2017 in Wuhan University, Wuhan, China, with a postal code of 430072 and a collection number of CCTCC NO: M2017510. A 16S rDNA sequence of the strain is shown as SEQ ID (sequence identifier) NO. 1. The bacillus amyloliquefaciens SXZ-N2 strain can be used for fermentation preparation of huperzine A and huperzine B. Microbial fermentation conditions are as follows: a formula of a culture medium comprises 5g / L yeast powder, 6g / L peptone, 5g / L sodium chloride and 8g / L sucrose; a pH (potential of hydrogen) is 8.0, culture conditions are as follows: 32 DEG C, 150r / min, and fermentation culture lasts for 10 days.

Description

technical field [0001] The invention relates to a strain of Bacillus amyloliquefaciens, which can produce Huperzine A and Huperzine B, and belongs to the technical field of microorganisms. Background technique [0002] At present, the research on plant endophytes has gradually received attention, and various endophytes of different plants have been discovered one after another. The diversity of endophyte species also endows the diversity of its metabolites, which also indicates that the use of microbial metabolism to explore new resources such as biology, chemistry, and medicine has great potential. A large number of studies have shown that endophytes can produce the same or similar active substances and precursors as host plants. This discovery directly promotes the extensive research on endophytes, especially medicinal endophytes. With the gradual research on medicinal plant endophytes, the use of endophytes that can metabolize and produce corresponding active ingredients...

AI Technical Summary

Problems solved by technology

However, due to the scarcity of Huperzia serrata plant resources, slow growth, and low content of huperzine A, only relying on the extraction of huperzine A from Huperzia serrata cannot meet the market demand. However, the activity of chemically synthesized huperzine A is far lower. It is better than natural huperzine A, and the cost of obtaining highly optically active huperzine A is relatively high

At present, there are some relevant reports in the field of using Huperzia serrata endophytes for microbial fermentation to produce huperzine A, but the types and yields of these strains still cannot meet the market demand for huperzine A

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