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Method for extracting huperzine A from serrate clubmoss herb

A technology of huperzine A and Melaleuca tower, which is applied in the field of extracting huperzine A from Melaleuca tower, can solve the problems of low yield, high cost, poor operability, etc., and achieve the purpose of improving leaching rate and increasing Solubility, the effect of process ease of operation

Active Publication Date: 2015-01-07
HUNAN HUACHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the defects of low yield, high cost and poor operability in the prior art, and provide a product with high yield, high purity, short extraction time, low cost and suitable Method for extracting huperzine A from Melaleuca tower for industrialized production

Method used

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  • Method for extracting huperzine A from serrate clubmoss herb

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] (1) Pre-treatment: Take dried Melaleuca medicinal materials (huperzine A content 0.053%), remove impurities, crush through a 20-mesh sieve, and take 10kg of crushed medicinal materials for use;

[0046] (2) Ultrasonic countercurrent extraction: add 60kg of water to the pulverized medicinal materials and soak twice, each time for 40 minutes, the soaking temperature is 50°C, discard the soaking liquid; send the soaked medicinal materials into the ultrasonic countercurrent extraction device, add 150kg volume Alkaline ethanol with a fraction of 90% and a pH value of 8.98 was extracted, the ultrasonic frequency was 60 kHz, the extraction temperature was 60°C, and the extraction time was 40 minutes. After extraction, it was filtered to obtain 147 kg of extraction solution;

[0047] (3) Membrane separation and purification: The extract obtained in step (2) was centrifuged with a butterfly centrifuge (DRY400, Liaoyang Shengfeng Pharmaceutical Machinery Co., Ltd.). Filtration, t...

Embodiment 2

[0055] (1) Pre-treatment: Take dried Melaleuca medicinal materials (huperzine A content 0.053%), remove impurities, crush through a 40-mesh sieve, and take 10kg of crushed medicinal materials for use;

[0056] (2) Ultrasonic countercurrent extraction: add 50kg of water to the pulverized medicinal materials and soak twice, each time for 30 minutes, the soaking temperature is 40°C, discard the soaking liquid; send the soaked medicinal materials into the ultrasonic countercurrent extraction device, add 100kg volume Alkaline ethanol with a fraction of 70% and a pH value of 9.05 was used for extraction, the ultrasonic frequency was 20 kHz, the extraction temperature was 50°C, and the extraction time was 30 minutes. After extraction, it was filtered to obtain 97.5 kg of extraction solution;

[0057] (3) Membrane separation and purification: The extract obtained in step (2) was centrifuged with a butterfly centrifuge (DRY400, Liaoyang Shengfeng Pharmaceutical Machinery Co., Ltd.). Fi...

Embodiment 3

[0065] (1) Pre-treatment: Take dried Melaleuca medicinal materials (huperzine A content 0.053%), remove impurities, crush through a 24-mesh sieve, and take 100kg of crushed medicinal materials for use;

[0066] (2) Ultrasonic countercurrent extraction: Add 540kg of water to the pulverized medicinal materials and soak twice, each time for 33 minutes, the soaking temperature is 45°C, discard the soaking liquid; send the soaked medicinal materials to an ultrasonic countercurrent extraction device, add 1260kg volume fraction Extract with 85% alkaline ethanol with a pH value of 8.96, the ultrasonic frequency is 40 kHz, the extraction temperature is 55 °C, the extraction time is 35 min, and the extraction is filtered to obtain 1227 kg of extraction solution;

[0067] (3) Membrane separation and purification: The extract obtained in step (2) was centrifuged with a butterfly centrifuge (DRY400, Liaoyang Shengfeng Pharmaceutical Machinery Co., Ltd.). Filter with an operating pressure o...

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Abstract

The invention discloses a method for extracting huperzine A from a serrate clubmoss herb. The method comprises the following steps: (1) pre-treatment; (2) ultrasonic countercurrent extraction: carrying out ultrasonic countercurrent extraction on the serrate clubmoss herb and filtering to obtain leach liquor; (3) membrane separation and purification: centrifuging, and carrying out ultrafiltration and nanofiltration on the leach liquor to obtain trapped fluid; (4) concentration, namely concentrating the trapped fluid into paste; (5) acid dissolution, dissolving the paste by using acid liquid, filtering, and adjusting pH to alkalinity; (6) extraction: extracting by using chloroform and collecting extract liquor; (7) concentration: concentrating the extract liquor to obtain dry powder; (8) crystallization and recrystallization: dissolving to obtain the dry powder, crystallizing and recrystallizing to obtain white crystals; and (9) drying, namely drying the white crystal to obtain a huperzine A product. According to the method disclosed by the invention, the purity of the huperzine A extracted from the serrate clubmoss herb is greater than or equal to 99%, the product yield is greater than or equal to 0.026%, the final yield is 50-55%, and the yield, yield coefficient and purity of huperzine A is high, the extraction time is short, and the cost is low; therefore, the method for extracting huperzine A from a serrate clubmoss herb is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for extracting huperzine A, in particular to a method for extracting huperzine A from a Melaleuca tower. Background technique [0002] Melaleuca pagoda (Huperzia serrata Trev.), also known as Huperzia serrata, is the whole herb of Huperzia serrata, a perennial herb of the genus Huperzia in the family Huperzia. It is one of the endemic plants in my country and is widely distributed throughout the country. Northeast China and the Yangtze River Basin, the whole herb can be used as medicine, and folks are often used to treat bruises, internal injuries, bleeding, stasis, swelling and pain, snake bites, etc. There are many modern studies on alkaloids in Melaleuca, among which huperzine A has attracted the most attention. It is considered to be a highly efficient, low-toxic, highly selective and reversible inhibitor of acetylesterase, which is effective for benign memory disorders. , Alzheimer's disease and myasthenia gravis ...

Claims

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Application Information

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IPC IPC(8): C07D221/22
CPCC07D221/22
Inventor 唐方华黄华学
Owner HUNAN HUACHENG BIOTECH
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