Copper complex, preparation method and application thereof

A technology of complexes and copper salts, applied in the field of copper complexes and their preparation, achieving the effects of low cost, high yield, and high purity

Inactive Publication Date: 2008-06-04
CAPITAL NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, so far, no copper complexes have been used clinically as anticancer drugs

Method used

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  • Copper complex, preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the preparation of complex

[0024] 0.2g Cu(NO 3 ) 2 ·3H 2 O and 0.15g Phen were added in 30ml DMF in turn, stirring constantly. After reacting for 8 hours, filter, diethyl ether diffuse, and stand at room temperature. After a few days, blue blocky crystals can be obtained with a purity of 99%.

[0025] Elemental analysis: Calculated (exp.): C 40.77 (40.76); H 3.51 (3.50); ​​N 15.86 (15.88). Infrared spectrum and X-ray diffraction single crystal structure diagram are shown in Fig. 1 and Fig. 2 respectively.

Embodiment 2

[0026] Embodiment 2, the preparation of complex

[0027] 0.2g Cu(CH 3 COO) 2 ·H 2 O, 0.2gNaNO 3 0.15g of Phen was sequentially added into a mixed solvent of 30ml of DMF and 10ml of methanol, and kept stirring. After reacting for 8 hours, filter, diethyl ether diffuse, and stand at room temperature. After a few days, blue blocky crystals can be obtained with a purity of 99%.

[0028] Elemental analysis: Calculated value (experimental value): C 40.77 (40.76); H 3.51 (3.50); ​​N 15.86 (15.88), its infrared spectrum and X-diffraction single crystal structure diagram are shown in Figure 1 and Figure 2 respectively.

[0029] Similar blue block crystals can be obtained by using other copper salts (such as copper sulfate, copper chloride, copper acetate, etc.), nitrates (such as nitrate, dilute nitric acid), DMF and solvents (methanol, acetonitrile, DMSO, etc.) —that is, the copper complex of the present invention.

Embodiment 3

[0030] Embodiment 3, cell experiment

[0031] (1) Experimental group:

[0032] Adherent tumor cells (A-549 (lung cancer) cells, Bel-7402 (liver cancer) cells, HCT (colon adenocarcinoma) cells) in the logarithmic growth phase were selected, digested with trypsin, and RPM11640 with 10% calf serum The culture solution was made into a cell suspension of 5000 cells / mL, inoculated in a 96-well culture plate, and inoculated 100 μL in each well, at 37°C, 5% CO 2 Cultivate for 24h.

[0033] Add 10 μL of the sample solution obtained in Example 1 (dissolve the complex crystal obtained in Example 1 with DMSO, and then dilute with saline to a final concentration of 5 μg / mL), the final volume of each well is 200 μL, and make up with 1640 culture medium. 37°C, 5% CO 2 Culture 3d.

[0034] After culturing for 3 days, discard the supernatant, add 100 μL of freshly prepared 0.5 mg / mL MTT (thiazolium blue) serum-free culture solution to each well, continue culturing at 37°C for 4 h, discard ...

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Abstract

The invention discloses a copper complex and the preparation method and application thereof, providing a cupric complex with 1, 10-phenanthroline, nitrate radical and N, N-dimethylfomamide. The preparation method of the cupric complex with 1, 10-phenanthroline is that nantokite, 1, 10-phenanthroline, nitrate radical and N, N-dimethylfomamide are mixed to react in solution to obtain the complex. The synthetic materials of the complex of the invention are easy to obtain and have low cost; the product precipitates in the form of crystalloid, thereby having a high purity and high production efficiency; besides, the complex can exist stably in natural states and has good water-solubility and fat-solubility; in addition, the complex of the invention has a good inhibiting effect on a plurality of cancer cells of lung cancer, liver cancer, adenocarcinoma of colon and so on, extensive effect and wide application range.

Description

technical field [0001] The invention relates to a copper complex and its preparation method, as well as the application of the complex in the preparation of medicines for preventing and treating cancer and tumor diseases. Background technique [0002] Copper is a trace element related to the maturation process of red blood cells, lymphocytes and bacteria-eating neutrophils. It plays a catalytic role in the formation of heme and red blood cells and participates in the formation of cells. Copper is also involved in the activities of more than 10 kinds of enzymes and important life hormones such as growth hormone, sex hormone, and pituitary hormone. It is also a component and catalyst of various enzymes in the human body. In addition, copper is also involved in the development of bones and the normal metabolism of the central nervous system related. Copper-containing enzymes include tyrosinase, monoamine oxidase, superoxidase, superoxide dismutase, ceruloplasmin, etc. Copper ...

Claims

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Application Information

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IPC IPC(8): C07F1/08A61K31/555A61P35/00C07D471/04
Inventor 鲁晓明王硕
Owner CAPITAL NORMAL UNIVERSITY
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