Application of plant possessing weed control activity
A herbicidal activity and plant technology, which is applied in the application field of plants with herbicidal activity, achieves significant social and economic benefits, and has a high growth inhibitory effect.
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[0015] 1. Extraction method
[0016] Weigh 20g of Chinese Torreya torreya in a 500ml Erlenmeyer flask with a stopper, add 200ml of 90% ethanol, extract at room temperature for 24h, and then extract with ultrasonic vibration for 1h. Continuously extract 3 times according to the above method, combine the 3 extractions, and then concentrate under reduced pressure below 50°C to form an extract. Use 90% ethanol to disperse the extract and settle to 80ml, and prepare the active test solution to be tested with a concentration equivalent to 0.25g dry sample / ml solvent.
[0017] 2. Bioassay method
[0018] Spread two layers of filter paper of appropriate size in a 9cm petri dish first, then add 2ml Chinese Torreya extract, after the solvent evaporates to dryness, add 3ml deionized water, and spot freshly germinated wild oats, barnyardgrass, retroflexus and 20 seeds of Artemisia persicae, each treatment was repeated 3 times, and 90% ethanol was used as a control. All treatments were ...
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