Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Oryzias melastigma fertilized egg hatching method

A technology of fertilized eggs and seawater, which is applied in the direction of climate change adaptation, application, fish farming, etc., can solve the problems of thickening and inevitable blackening of fertilized eggs, and achieve the effect of high cleanliness

Active Publication Date: 2020-05-12
山东省海洋科学研究院青岛国家海洋科学研究中心
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These two hatching methods can effectively save water and obtain larvae, but no matter which method, it is inevitable that the fertilized eggs will turn black, thicken, and die in the later stage of hatching

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Oryzias melastigma fertilized egg hatching method
  • Oryzias melastigma fertilized egg hatching method
  • Oryzias melastigma fertilized egg hatching method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A kind of efficient seawater medaka (Oryzias melastigma) fertilized egg incubation method is characterized in that:

[0026] The physicochemical conditions of described incubation method are as follows:

[0027] The experimental seawater is taken from non-polluted seawater in the field. Before use, it needs to be filtered by sediment sand and absorbent cotton. The salinity is 30±1‰; the hatching temperature is 26-28°C; the air humidity is about 40-60%; Large, 80-125μmol·m -2 ·s -1 It is more conducive to the hatching of fertilized eggs; the light cycle is 14:10, and the lighting period is from 6 am to 8 pm.

[0028] The hatching process of the fertilized eggs diluted in the salinity gradient is as follows:

[0029] Step 1. Place the broodstock with eggs in a petri dish covered with absorbent cotton, carefully suck the fertilized eggs with a plastic straw, and move them to the dissecting microscope for observation. Select the normally fertilized zygotes and transfer ...

Embodiment 2

[0036] The fertilized eggs of medaka hatched in pure seawater were used as the control group. The physical and chemical conditions for hatching were exactly the same as in Example 1. Natural seawater filtered by absorbent cotton was used throughout the hatching process, with a salinity of 30±1‰. In the later stage of hatching, a large number of fertilized eggs were adhered by microalgae ( figure 2 ), the egg shell of the fertilized egg thickens and turns black, and the surface filaments will adhere to residual bait, feces and other particulate matter in the water body ( image 3 ), causing some larvae to emerge from the membrane, only the tail trembled out, the head was stuck in the membrane, and finally died due to hypoxia ( Figure 5 ). The hatching rate of the pure seawater control group is only about 67%, significantly lower than that of Example 1. At the same time, compared with the almost simultaneous hatching of larvae in Example 1, the hatching period of fertilized ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to an oryzias melastigma fertilized egg hatching method, and belongs to the field of aquaculture. According to the oryzias melastigma fertilized egg hatching method, the fertilized eggs are hatched in seawater with different salinity gradients, the physical and chemical conditions for hatching are the temperature being 26-28 DEG C, the humidity being 40-60%, the light intensity being 80-125[mu]mol.m-2.s-1, and the light-dark ratio being 14:10. The fertilized eggs with high cleanliness can be obtained, experimental operations such as embryology experiment observation and microinjection can be carried out conveniently, and the hatching rate of the fertilized eggs can be as high as 94.7% by using the oryzias melastigma fertilized egg hatching method. Moreover, hatched oryzias melastigma can develop, grow and reproduce normally after the long-term observation, and no abnormal phenomena are found.

Description

technical field [0001] The invention relates to the field of fish breeding, in particular to a method for efficiently hatching fertilized eggs of marine model organism medaka. Background technique [0002] Seawater medaka (Oryzias melastigma) belongs to the phylum Chordate, the class Radix, the order Cleptichthyes, the family Oryzias melastigma, and the genus Oryzias. Its individual is small, and the adult body length is only 28±2mm, which is easy to cultivate artificially in the laboratory. The reproductive cycle is short and sexual maturity can be reached at the age of 3 months. The diameter of the fertilized egg is about 0.9±0.05mm, and the overall color is transparent, which is convenient for observation. Due to the above-mentioned biological advantages, seawater medaka has been recommended as an ideal marine model fish species, and is widely used in biological development, ecotoxicology and molecular biology research. [0003] The spawning type of medaka in seawater ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/17
CPCA01K61/17Y02A40/81
Inventor 于道德宋静静盖珊珊刘凯凯迟雯丹
Owner 山东省海洋科学研究院青岛国家海洋科学研究中心
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com