Live embryo surface zona pellucida tension detection device

Abstract

The invention discloses a live embryo surface zona pellucida tension detection device. The device comprises an inverted microscope, further comprises a pressure sensor which is fixedly connected witha first operating arm of the inverted microscope, an extrusion needle which is fixedly connected with the other end of the pressure sensor and is used for extruding an embryo, a fixed needle which isfixedly connected with a second operating arm of the inverted microscope, and a micromanipulation vessel which is positioned on an objective table of the inverted microscope, wherein the pressure sensor is electrically connected with the computer through a data line, and the pressure sensor transmits detected data to the computer for storage. The device is advantaged in that the pressure sensor isadditionally arranged on an existing inverted microscope, a camera on the inverted microscope is combined, collection and research of tension-related data of a transparent belt on a surface of a tested living embryo are facilitated, a separation blade is arranged in the micromanipulation vessel so that a supporting surface is provided for the living embryo conveniently, a zona pellucida on the surface of the living embryo is slightly deformed under the combined action of the extrusion needle, the fixed needle and the separation blade, the living embryo is not damaged, finally, a tension coefficient of the living embryo is calculated, and embryologists can judge activity of the living embryo by using data and common indexes for embryo judgment conveniently.

Description

Technical field [0001] The invention relates to the technical field of medical equipment, in particular to a zona pellucida tension detection device on the surface of a living embryo. Background technique [0002] The usual test-tube baby is called assisted reproductive technology in the industry. A traditional assisted reproductive technology includes in vitro fertilization. When the patient’s egg is surgically removed after ovulation induction, the egg is combined with the husband’s sperm outside the body. After 3-6 days of culture, the embryo You can choose to be transplanted back to the mother to complete the assisted conception process, but the research data shows that if the patient is more than 35 years old, the zona pellucida on the surface of the patient's embryo will become too strong (commonly known as the zona pellucida), making it difficult for the embryo to hatch in the mother. It requires assisted incubation. The laser method is now more popular. Install the laser ...

AI Technical Summary

Problems solved by technology

[0002] The usual test-tube baby is called assisted reproductive technology in the industry. A traditional assisted reproductive technology includes in vitro fertilization. When the patient's eggs are surgically removed after ovulation induction, the eggs are combined with the husband's sperm outside the body. After 3-6 days of culture, the embryos You can choose to be transplanted back to the mother to complete the assisted conception process, but the research data shows that if the patient is older than 35 years old, the tension of the zona pellucida on the surface of the patient's embryo will become too large (commonly known as the zona pellucida is too hard), making it difficult for the embryo to hatch in the mother's body. Assisted hatching is required, and the laser method is more popular now. Install a laser module on the microscope, observe the image of the embryo on the computer screen, aim at the zona pellucida of the embryo, open the zona pellucida, and then transplant it back to the mother. Holes may cause some damage to the embryos. The laser will generate high temperature, which may cause some bad chemical changes. In addition, whether the embryos are over 35 years old or whether the embryos have been frozen may be used as a basis for assisted hatching. People may have less surface tension of the embryo, the embryo is softer, and does not need assisted hatching, while people younger than 35 years old may have an unsatisfactory zona pellucida, which is relatively hard, and may even need assisted hatching

Images