The invention discloses a
cell seedling temperature
cryopreservation method and a device, and belongs to the technical field of
cell cryopreservation, the method comprises the following steps: S1, cooling a
cryopreservation tube loaded with cells to be cryopreserved through a
refrigeration system according to a preset
cooling rate, and detecting the temperature of the cryopreservation tube in the cooling process; S2, judging whether the temperature of the cryopreservation tube reaches a preset
seedling temperature or not, if yes, executing S3, and if not, executing S1 continuesly; S3, adopting the
refrigeration system to conduct heat preservation on the cryopreservation tube at the preset
seedling temperature; S4, applying ultrasonic
waves to the cryopreservation tube through an ultrasonic vibrator so as to carry out seedling temperature operation, and lasting the operation for preset threshold time; S5, judging whether seedling temperature operation of the cryopreservation tube succeeds or not by analyzing the temperature of the cryopreservation tube before and after the seedling temperature operation, if yes, executing S6, and if not, continuing to execute S4; and S6, cooling the cryopreservation tube continuesly through the refrigerating
system, stopping cooling after the cryopreservation tube is cooled to the preset cryopreservation temperature, and carrying out heat preservation. According to the method, the
cell cryopreservation effect is improved by adding the step of judging whether seedling temperature operation succeeds or not.