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Sheep seminal fluid cryoprotectant

A cryoprotectant and cryopreservation technology, applied in the field of animal reproduction, can solve problems such as reducing sperm survival rate and affecting conception rate

Inactive Publication Date: 2021-04-20
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, sheep semen contains an enzyme that will react with the egg yolk in the diluent, reducing the survival rate of sperm, thus affecting the conception rate

Method used

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  • Sheep seminal fluid cryoprotectant
  • Sheep seminal fluid cryoprotectant
  • Sheep seminal fluid cryoprotectant

Examples

Experimental program
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Effect test

Embodiment 1

[0028] 1 Materials and methods

[0029] 1.1 Test material

[0030] 1.1.1 Test animals

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Abstract

The invention discloses a sheep seminal fluid cryoprotectant and an application thereof, wherein the sheep seminal fluid cryoprotectant comprises a freezing base diluent and an AMPK activator, and the AMPK activator is Met or AICAR. By adding the AMPK activator Met or AICAR with a proper concentration into the sheep seminal fluid cryopreservation diluent, the vitality, movement performance and structural integrity of unfrozen sperms can be improved by activating AMPK, and the seminal fluid quality is improved by maintaining acrosin activity, improving antioxidant enzyme activity, promoting sperm metabolism and maintaining mitochondrial functions.

Description

technical field [0001] The invention belongs to the technical field of animal reproduction, and in particular relates to a sheep semen cryoprotectant and application thereof. Background technique [0002] Semen cryopreservation technology can break the barriers of time and space to preserve semen for a long time, improve the utilization rate of high-quality rams, and speed up the process of breed improvement. It is one of the key links of artificial insemination technology. In recent years, scholars at home and abroad have researched sheep semen cryopreservation technology from different angles, enriching the data in this field, but the study of frozen semen technology started late in the field of sheep. At present, the viability of sheep frozen semen after thawing is higher than that of other livestock (such as cattle and pigs) is still at a low level, and the conception rate of artificial insemination using frozen semen is also lower than that of natural mating. The plasm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 张艳丽汪棋万永杰王锋
Owner NANJING AGRICULTURAL UNIVERSITY
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