89results about How to "Increase lactic acid content" patented technology

The invention discloses an animal feed additive and a preparation method thereof. The animal feed additive is prepared from the following raw materials in parts by weight: 5-10 parts of corn fiber, 15-20 parts of soybean curb residue, 12-15 parts of zeolite powder, 40-60 parts of corn sugar residue, 1 part of compound biological active enzyme, 1 part of saccharomycetes, 1 part of lactobacillus, 1 part of bacillus and 1 part of bifidobacterium, wherein the compound biological active enzyme comprises the following components: 20,000 IU / g of xylanase, 10,000 IU / g of pectinase, 400 IU / g of cellulose and 200 IU / g of protease. The preparation method comprises the steps of raw material weighing, pre-processing, mixing, dual fermentation and packaging. The animal feed additive disclosed by the invention can be used for improving the digestibility to promote the growth and development of animals, regulating the gastrointestinal micro-ecological balance to enhance animal immunity and improve disease resistance, improving animal feed intake, improving the cultivation environment to reduce respiratory diseases, and improving the quality and quantity of cultivated products.

The invention belongs to the technical field of food and relates to health vinegar, a health vinegar beverage rich in lactic acid bacteria and a preparation method thereof. The health vinegar beverage is prepared according to the following steps: by taking black rice as a main material, pasting, saccharifying, mixing yeast, performing alcoholic fermentation, performing acetic fermentation, smoking and fermenting, pouring vinegar, and the like. In the brewing process, lactic acid bacteria, selenium-enriched yeast and acetic bacteria are used for cooperatively fermenting, so that the lactic acid content in the product is promoted and the product flavor and taste are improved. The selenium-enriched yeast can increase the organic selenium content in the product, can promote anti-oxidation effect and can enhance the immunity of the organism and the function of preventing tumor. Active lactic acid bacteria are added and mixed into the vinegar beverage in the later period, so that the vinegar beverage can synergically exert the anti-oxidation effect and can adjust the enteric microbial flora balance. In the product, the taste of the vinegar and the vinegar beverage can be improved, the category and content of functional components are increased, the healthcare effect is well exerted and the market requirement is met.

The invention discloses a microbial inoculum for preparing feed and application thereof. The active components of the microbial inoculum comprise Lactobacillus plantarum, Lactobacillus kimchii, Lactobacillus farciminis and Lactobacillus pentosus. The four inocula in the lactic acid bacteria composite system of the microbial inoculum of the invention have high synergetic effect, can rapidly reproduce, colonize and dominate in an unpasteurized natural raw material, effectively control microbiota in silage, rapidly generate a large amount of lactic acid, reduce the pH value of the feed to about 4.0, reduce the dry matter loss and the feed quality decline caused by growth of contaminated microbes in the silage period to the farthest extent, reduce the generation of ammonia nitrogen, and improve the fermentation quality of the silage. Compared with control, the lactic acid production amount and speed in the alfalfa silage taking the microbial inoculum of the invention as an additive are obviously increased, and the protein content, the dry matter content and the like of the obtained alfalfa feed are obviously improved.

The invention relates to a rice straw ensilage method, which comprises the following steps of: 1) cutting reaped bluish yellow rice straws into 8-10cm small sections; 2) piling the cut rice straws into an ensilage silo, compacting the piled rice straws once per every 100m<2>, simultaneously spraying ensilage lactic acid bacteria starters, thereafter continuously piling the rice straws and spraying the ensilage lactic acid bacteria starters till the ensilage silo is full; and 3) after the rice straws are ensilaged, laying plastic films around an ensilage stack for sealing, compacting the ensilaged straws at the same time and putting sand bags on the ensilage stack after the ensilage stack is comprehensively sealed. The rice straw ensilage method is simple to operate; and by adding the special ensilage lactic acid bacteria starters to ensilage the rice straws, the pH value of the ensilaged rice straws is reduced, the content of lactic acid is improved and the rice straws with poor palatability and fewer usable nutrients are made into ensilage feeds with a high feeding value.

The present invention provides a production method for elymus nutans griseb silage. The method comprises the following steps: 1) preparing a composite silage agent; 2) preparing before silage; 3) timely harvesting and regulating water; 4) packing, compacting and spraying silage agent; 5) overall sealing the silage heap, covering soil on the side and top and compacting the soil. Compared with the conventional silage method, the present invention not only can reduce the dry matter and nutrient loss of silage, but also can rapidly decrease the PH, increase the lactate content, and has significant effect of improving the quality of silage.

The invention belongs to the field of utilization of waste biomass resources, and discloses a pretreatment method capable of improving crop straw biomass anaerobic digestion rate, which mainly solves the problems of longer anaerobic digestion cycle, low digestion rate and the like of the existing crop straw. According to the invention, the straw is directly bundled and stored in the field, so the land occupation area of the methane engineering is reduced, the investment is reduced, and the like; meanwhile, the methane yield can be increased, and the economic benefits are good. The pretreatment method particularly comprises the following steps: after the integral straw is harvested, naturally airing the straw in the field to enable the water content to reach 35% to 40%; carrying out chemical treatment by a fermentation inhibiting agent, wherein the usage amount of the fermentation agent is 300 to 400 ml per 100 kg of a raw material, and reducing the pH value; after acidifying, mechanically compacting and bundling, wherein the straw bundling density is about 130 to 140 kg / m3; coating and sealing the straw by thin films, and storing for 20 to 30 days; taking the slightly stored straw and inoculating silt, inoculating according to the ratio of 1:10, and carrying out the anaerobic digestion reaction, wherein the reaction temperature is 38 to 40 DEG C and the reaction time is 15 to 20 days. The technique has broad application prospect in the straw methane engineering.

The invention discloses a corn straw silage feed preparing technology. A corn straw silage feed is prepared through the steps of preparing a silage pond, collecting and sorting silage feed raw materials, performing cutting-up, adding a feed additive, performing loading, closing over dry substances, performing film coating pretreatment, and performing earth coating for sealed storage. According tothe corn straw silage feed prepared by the corn straw silage feed preparing technology disclosed by the invention, the content of a hazardous substance, namely flavatin, in the feed is reduced, the mildew and rot rate of the feed is reduced, and the mouth feel and the quality of the feed are improved; besides, the silage feed is not liable to decompose due to silage, the silage loading void content is reduced, and favorable anaerobic environment can be created for lactic acid bacteria fermentation, so that fermentation is facilitated; a layer of the dry substances are covered, so that the situation that a large quantity of nutrient components run off along with moisture is avoided, nutrient loss is reduced, and the fragrance of lactic acid of the silage is improved; and the feed additive is added, so that the quality of the silage feed can be improved, the food consumption of the feed is increased, and the palatability of the feed is strengthened.

The invention relates to the field of application of microbial fermentation, in particular to low-acidity micro-ecological food produced through three-stage probiotic fermentation of Chinese dates anda preparation method of the low-acidity micro-ecological food. The invention mainly provides probiotic functional beverages, probiotic powder, the micro-ecological food and preparation methods of theprobiotic functional beverages, the probiotic powder and the micro-ecological food through staged fermentation based on different temperatures with agricultural products such as the Chinese dates andsoybeans as raw materials. The invention aims to prepare the probiotic functional beverages, the probiotic powder and the micro-ecological auxiliary food for intestinal flora disorder, constipation and diarrhea of modern people, and an idea is provided for production and research of related products.

The invention discloses a preparation method of ashitaba tea. The preparation method comprises the following steps: processing tender shoots of ashitaba by steaming blanching; hydrating; fermenting with lactic acid bacteria; drying at low temperature; rolling; and drying. With the adoption of the preparation method, the grass smell of ashitaba is fundamentally removed, the bad taste like astringency and piquance can be avoided; and when brewing, the ashitaba tea soup is clear and transparent, faint scent, tastes pure and mild, and has a good drinking effect. The method is simple in process, low in preparation cost, out of environmental pollution, and beneficial for industrial production, and brings a new method for effectively utilizing ashitaba.

The invention relates to piglet feed and especially relates to fermented type mixed feed for piglets, which is prepared reasonably after feed raw materials subjected to fermentation treatment are added. The fermented type mixed feed for piglets comprises the following components in parts by weight: 584.19-651.79 parts of corn, 178-200 parts of bean meal, 19-31 parts of wheat bran, 50-90 parts of fermented raw materials, 25-30 parts of sucrose, 20-40 parts of fishmeal, 5-8 parts of stone powder, 8-12 parts of soybean oil, 8-12 parts of calcium hydrogen phosphate, 3.0-3.5 parts of sodium chloride, 1.0-1.5 parts of choline chloride, 0.6-0.8 part of methionine, 3.5-4.0 parts of lysine hydrochloride, 1.0-1.3 parts of threonine, 0.26-0.34 part of tryptophan, 0.15-0.17 part of phytase, 0.9-1.1 parts of trace element premixed feed, 1.0-1.1 parts of vitamin premixed feed and 0.4 part of probiotics for pigs. The fermented type mixed feed for piglets has long-term economic benefits for feed production enterprises and piglet raisers and also can generate huge environmental protection benefits for the society.

The invention discloses lactobacillus amylovorus for producing feruloyl esterase. Named as (lactobacillus amylovorus)c, the bacterial strain is preserved in China General Microbiological Culture Collection Center on July 6th, 2015 with the preservation number of CGMCC No.11056. Tests show that lactobacillus amylovorus can produce amylase when growing in a starch-containing culture medium and can produce feruloyl esterase when growing in an ethyl-ferulate-containing culture medium, that is, lactobacillus amylovorus has a potential application value in paper-making, textile, food, cosmetics and pharmaceutical industries, particularly, a lactobacillus amylovorus fermented silage can improve the content of lactic acid by 8%, reduce the pH value by 0.27 and shorten the fermentation period by 15 days, and lactobacillus amylovorus has a wide application prospect in preparation of fermented silages and other microecologics.

The invention relates to a method for preparing corn straw coarse feed by microbial beneficial living bacteria. The method comprises the steps of (1) preparing microbial strains: respectively feeding two strains of bacillus subtilis, one strain of lactobacillus acidophilus, one strain of lactobacillus bulgaricus and one strain of rhodopseudomonas palustris onto corresponding culture mediums, culturing, carrying out expanding propagation, and mixing 20g of bacillus subtilis, 20g of lactobacillus acidophilus, 20g of lactobacillus bulgaricus and 20g of rhodopseudomonas palustris for later use; preparing 100g of cellulase; (2) preparing corn straw: flattening the corn straw, and drawing into hair shape with a wire drawing machine for later use; (3) preparing the corn straw coarse feed: putting 1000g of prepared corn straw into a fermentation container, and evenly spraying 5g of cellulase onto the corn straw; diluting microorganism with well water, then spraying onto the corn straw, and fermenting in an airtight way for 7-10 days; taking out the product, drying and packaging to obtain the corn straw coarse feed. The method is simple in technology and short in fermentation period, and is capable of saving a great deal of grain; the prepared biological straw feed is good in palatability, high in lactic acid content and more in beneficial bacteria.

The invention discloses a high plant cell wall degradation activity rumen fungi, and applications thereof in feed ensiling, and belongs to the technical field of microorganism and feed processing. According to a preparation method, Hungate anaerobic roll-tube technique is adopted to obtain an anaerobic fungi with cell wall degradation enzyme activity via separating and selecting from the rumens of Xinong Saanen dairy goats, finally obtained Piromyces sp.CN6 is capable of producing xylanase and acetylesterase with relatively high activity in a wide temperature and pH range, and excellent thermal stability and acidic and alkaline stability, the preservation number is CGMCC No.14449. The bacteria strain is capable of improving fermentation quality of silage, increasing crude fiber degradation rate, and can be applied in practical production as a silage additive.

The invention discloses a recirculating breeding method of organic chickens using black soldier flies and green source bacteria. The method comprises a closed organic circulating mode composed of the steps of breeding of the black soldier flies which specially feed on chicken manure, culturing of the green source bacteria, processing of black soldier fly and green source bacterium organic fermented fodder and breeding and feeding of the organic chickens. Breeding of the black soldier flies which specially feed on the chicken manure refers to the fact that the chicken manure of the organic chickens is dried and crushed into chicken manure residue, minor ingredients are added and mixed with the chicken manure residue to obtain culture materials, black soldier fly eggs are inoculated onto the surface of the culture materials and cultured into mature larvae, and the black soldier fly mature larvae are collected; culturing of the green source bacteria refers to the fact that lactobacilli and Lactic acid bacteria supplementary to the lactobacilli are combined into the Lactic acid bacteria with an ultrahigh compound character; the black soldier fly and green source bacterium organic fermented fodder refers to the fact that the collected black soldier fly mature larvae are regarded as main materials, and the cultured green source bacteria are added for fermentation to obtain the fodder. By means of the method, the purposes of easy operation, easy achievement, high efficiency and high earnings are achieved for people, operation is simple, the cost is low, the transformation rate of waste material utilization is high, and the environmental protection effect is good.

The invention discloses a plant lactobacillus and an application thereof in the ensilage of warm-season forage, and belongs to the field of processing and storage of forage. The provided strain (LP1) is named as Lactobacillus plantarum. The preservation number of the strain in the China General Microbiological Culture Collection Center (CGMCC) is CGMCC No. 10473. The plant lactobacillus is separated from natural herbage silage and is a novel strain of plant lactobacillus. The biological additive prepared from the plant lactobacillus and cellulase can prominently improve the fermentation quality of ensilage of warm-season forage, has the advantages of high efficiency and no pollution to the environment, does not have any adverse influence on livestock, can be easily mastered during the addition process, and can be widely used in the field of warm-season forage silage preparation.

The invention provides a liquid bacterial liquid for fermenting a piglet liquid feed, and a preparation method and application of the piglet liquid fermented feed. The liquid bacterial liquid for fermenting the piglet liquid feed comprises a bacillus liquid and a lactic acid bacteria liquid; the bacillus liquid is a mixed bacteria liquid of bacillus licheniformis and bacillus subtilis; and the lactic acid bacteria liquid is a mixed bacteria liquid of lactobacillus plantarum and enterococcus faecalis. The piglet liquid fermented feed is a feed fermented by adopting the liquid bacterial liquid for fermenting the piglet liquid feed. The liquid bacterial liquid provided by the invention can improve the utilization rate of the feed, improve the flavor of the feed and improve the palatability, and after the feed is fermented, the contents of reducing sugar and lactic acid are obviously increased, the probiotic number is obviously increased, and the feed has acid fragrance. The piglet liquidfermented feed contains a large amount of probiotics, and is mainly used for enhancing the immune function of the piglets, killing pathogenic microorganisms and preventing diseases such as diarrhea ofthe piglets by fermenting to generate lactic acid and secrete some antibacterial substances.

The invention discloses a multi-strain liquid fermented vinegar rich in lactic acid and acetoin produced through multi-strain fermentation. A brewing method comprises the following steps: during a brewing process, firstly, soaking and cooking rice or sticky rice; adding water and then adding liquifying enzyme and saccharifying enzyme, thereby acquiring a saccharification liquid; inoculating with abrewer yeast for fermenting, thereby acquiring wine with dregs; mixing bran with water, and then filtering, thereby acquiring a bran leachate; inoculating lactobacillus helveticus, thereby acquiringa lactic acid fermented liquor; mixing the lactic acid fermented liquor with the wine with dregs, and inoculating with pasteur acetate and acetobacter gluconicum for acetic fermentation, thereby acquiring a vinegar liquor rich in acetoin; adding saccharose and inoculating lactobacillus acidophilus for anaerobic fermentation, thereby acquiring the liquid fermented vinegar rich in lactic acid and acetoin. The increasing of lactic acid content is capable of enhancing softness of vinegar and reducing excitement; in an ageing stage, acetoin is capable of reacting with the free amino acid in the bran leachate, thereby generating ligustrazine, so that the multi-strain liquid fermented vinegar has a fragrance of baking nuts and has healthcare effects of protecting heart and cerebral vessels, resisting against oxidation, softening blood vessels, and the like; besides, the free amino acid in the bran leachate is beneficial to enhancing freshness of liquid vinegar.

The invention discloses a method for brewing blending mature vinegar by applying an immobilized targeted multi-microorganism strain. The method comprises the following steps of: carrying out co-immobilization treatment on the targeted strains separated out from the yeast for making hard liquor, and then adding the cultured seed mash obtained through the cultivation and fermentation of the co-immobilization strains instead of part of yeast for making hard liquor to the liquid state thick mash alcoholic fermentation stage and solid state alcoholic fermentation process for mature vinegar brewing,wherein the co-immobilization strains are alternated and subjected to enzyme system complementary in the fermentation process and are synergistically fermented in the alcohol fermentation stage and the acetic fermentation stage, so that metabolites are rich in the end of fermentation, and the mature vinegar is sour, soft and palatable and has mellow taste.

The invention discloses lactococcus lactis ZF625 which is preserved in Guangdong Province Microbiological Culture Collection Center on 12 December 2018 with the preservation number GDMCC No:60522. Theinvention further discloses the use of lactococcus lactis ZF625 to food fermentation. Through mutation screening, the lactococcus lactis ZF625 which is resistant to ethanol and high in yield of lactic acid is obtained; when the lactococcus lactis ZF625 is used for brewing vinegar by a liquid state fermentation method, the lactic acid content in the vinegar can be significantly increased, the flavor of the vinegar can be improved, the irritant of the sourness of the vinegar can be reduced, and the mellow sense can be promoted.

The invention relates to a zein hydrolysate and a preparation method and application in brewing vinegar with pure grains thereof. The preparation method includes the steps of mixing corn gluten meal and water in a mass ratio of 1:8 to 1:10, adjusting pH to 7.5 to 9.0, adding protease, performing enzymolysis for 24-48 hours at the temperature of 50-60 DEG C to obtain a zein enzymatic hydrolysis solution; adding glutaminase into the zein enzymatic hydrolysis solution, performing hydrolysis for 12-24 hours at the temperature of 45-55 DEG C to obtain a deamidization zein enzymatic hydrolysis solution; performing enzyme deactivation of the deamidization zein enzymatic hydrolysis solution, centrifuging the solution, getting a supernatant, and performing freeze-drying to obtain the zein hydrolysate. The zein hydrolysate is prepared from corn gluten meal by the multienzyme enzymatic hydrolysis, and can be used for brewing vinegar with pure grains to improve the lactic acid content in the edible vinegar significantly, so that the mouthfeel of the edible vinegar and the efficiency of acid production are improved.

The invention discloses bacterium liquid used for fermenting cassava alcohol residue feed. The bacterium liquid is formed by combining one or more of spore bacteria and lactic acid bacteria. The inoculation proportion of the spore bacteria to the lactic acid bacteria is 3:2 (v / v), the inoculation proportion of the spore bacteria B1 to the spore bacteria B2 is 1:2 (v / v), and the inoculation proportion of the lactic acid bacteria R1 to the lactic acid bacteria R2 is 1:2-3 (v / v). The invention further discloses application of the bacterium liquid used for fermenting the cassava alcohol residue feed to improving feed flavor and improving palatability during preparation. Experimental results show that the number of probiotics of the cassava alcohol residue feed fermented by the bacterium liquid is obviously increased; the content of lactic acid is obviously increased through fermentation of the cassava alcohol residue feed; the fermented cassava alcohol residue feed has the obvious sour and sweet smell.

The invention discloses lactobacillus acetotolerans. The lactobacillus acetotolerans is preserved in the China General Microbiological Culture Collection Center, the preservation date is 13 December 2018, and the preservation number is CGMCC NO.16938. The invention further discloses an application of the strain. The lactobacillus acetotolerans disclosed by the invention is high in tolerance to acetic acid, can convert carbohydrate substances into the lactic acid in high acetic acid environment of which the acetic acid concentration is 7.0g / 100mL, and can produce fragrance substances of ethyl lactate and the like. After the strain is used in brewed liquid vinegar of which the total acid is 6.0-7.0g / 100mL, the acid content can be increased to 0.76-0.91 / L, the ethyl lactate content can be increased by 10 times or above and reach 13.69-15.26mg / L, the irritant of products is reduced, the softness is increased, the content of fragrance substances is increased, the flavor quality of the products is greatly improved, the application is convenient, and economic benefits of enterprises are significantly increased.

The invention discloses king grass-pineapple peel mixed silage. The king grass-pineapple peel mixed silage is characterized by being composed of the following raw materials in percentage by weight: 70%-90% of king grass and 10%-30% of pineapple peels. The king grass-pineapple peel mixed silage has the effects that the water content of the king grass is reduced by virtue of air-drying treatment, and the pineapple peels are added to supplement soluble sugar of a silage fermentation substrate so that king grass ensiling succeeds easily; due to the treatment, the pH value and the content of ammonium nitrogen of the silage are reduced, the content of lactic acid in the silage is increased, the quality of ensiling is improved obviously, the content of fibers is reduced and the nutritional value of the king grass silage is increased; an effective king grass ensiling method provided by the invention is simple to operate, and has important significance for solving the problems of unbalanced supply of roughage resources and the like, reasonably utilizing industrial by-products and improving ecological environment.

The invention belongs to the technical field of animal feed, and relates to mixed silage and a preparation method. The mixed silage comprises a forage crop and a silage inoculant, wherein the forage crop comprises forage sweet sorghum, sorghum sudanense and silage corn, and the silage inoculant comprises any one or a combination of at least two of FHI, MMD3 and FHIIL. The preparation method of themixed silage comprises the steps of mixing the forage crop with the silage inoculant for silage and fermentation to obtain the mixed silage. The mixed silage is high in crude protein content, high incrude fat content and high in digestibility, and facilitates livestock rumen degradation; and the added silage inoculant regulates a silage microflora, promotes lactic acid bacterium fermentation andimproves the quality and palatability of the silage. A technical support is provided for solving the problems of roughage shortage and storage, so that the effective supply of livestock products is further guaranteed.

The invention relates to a method for mixed fermentation of probiotics containing bacillus coagulans. The method comprises the following four steps: rejuvenation and activation of a culture, seed solution configuration of a configured liquid medium, secondary culture and solid material production. On the one hand, a processing and production technology is simple, a production process is standard,the production efficiency and quality can be improved effectively, the production cost is reduced, on the other hand, the bacillus coagulans, saccharomyces cerevisiae and lactobacillus bulgaricus arefermented in a mixed manner creatively, the content and palatability of lactic acid are greatly improved, PH of a fermented finished product is between 4.0 and 4.5, and the content of the bacillus coagulans is greater than or equal to 109 CFU / mL; the content of the saccharomyces cerevisiae is greater than or equal to 108 CFU / mL; and the content of lactic acid bacteria is greater than or equal to 108 CFU / mL.

The invention discloses a preparation method of rosa roxburghii tratt fruit vinegar through mixed bacteria compound fermentation. The preparation method comprises the following steps: (1) preparing roxburgh rose juice: screening roxburgh roses, putting the roxburgh roses into a pulping machine for smashing, filtering and sterilizing to obtain the roxburgh rose juice; (2) dilution: adding water to dilute the roxburgh rose normal juice to obtain a roxburgh rose juice diluent; (3) adjusting sugar degree: adding white granulated sugar into the roxburgh rose juice diluent to obtain a sugar-containing roxburgh rose juice diluent; (4) alcoholic fermentation: activating yeast, inoculating the yeast into the sugar-containing roxburgh rose juice diluent, activating lactic acid bacteria, and inoculating and fermenting to obtain roxburgh rose fruit wine; and (5) acetic fermentation: activating acetic bacteria, inoculating the activated acetic bacteria into the rosa roxburghii tratt fruit wine, and fermenting to obtain the mixed bacteria composite fermented rosa roxburghii tratt fruit vinegar. The rosa roxburghii tratt fruit vinegar integrating the functions of nutrition, health care, dietary therapy and the like is prepared by jointly fermenting the rosa roxburghii tratt fruit juice with the saccharomycetes and various lactic acid bacteria, and the taste and quality of the rosa roxburghii tratt fruit vinegar are improved.

The invention discloses a feed for non-ruminant animals and a preparation method thereof. The feed comprises 30-60 parts of cassava, 20-30 parts of cassava stalks, 7-17 parts of pigeon pea stems and leaves, 1-2 parts of bacterial powder, 3-5 parts of yam beans, 2-5 stems and leaves of black nightshade, 2-4 parts of urea and 5-7 parts of beet pulp; wherein the stems and leaves of the black nightshade are soaked in water at the temperature of 45-75 DEG C; the pigeon pea stems and leaves and the yam beans are soaked in an ethanol water solution at 65-80 DEG C, and the ethanol water solution contains 5-10% of sodium formate. The silage provided by the invention has aromatic smell, is close to the raw materials in color and luster, is light brown after being dried, stems and leaves structure are kept well, and reaches an excellent grade. The dry matter content in the feed is 44-47%, the crude protein content is 5.5-6.5%, the soluble sugar content is 528-584 g / kgDM, the lactic acid content is 5-9%, the acetic acid content is 2-5%, the propionic acid content is 0.2-0.3%, butyric acid and hydrocyanic acid are not detected, the ammoniacal nitrogen content is as low as 0.023 g / kgDM, the pH is 3.09-3.80, the neutral detergent fiber content is 10-22%, the acid detergent fiber content is 5-12%, the feed quality is excellent, and the requirements of non-ruminant animals are met.

The invention provides a polysialic acid fermentation culture medium and a method for producing polysialic acid through escherichia coli fermentation. The polysialic acid fermentation culture medium is a total synthesis fermentation culture medium; compound amino acid is creatively used as a nitrogen source of the fermentation culture medium; problems like escherichia coli apoptosis in advance inthe fermentation process due to the facts that traditional natural organic matters like corn steep liquor dry powder is high in lactic acid content, and metabolic pressure is applied to the escherichia coli in the fermentation process, especially in the large-scale fermentation process are avoided; in addition, the compound amino acid culture medium can improve a material conversion rate and prevent metabolic abnormality of the escherichia coli; and finally, the yield of the polysialic acid can be increased after the production scale is enlarged, and good process consistency is achieved.

The invention discloses lactobacillus plantarum CV10D1 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) on March 20, 2018, the preservation number is CGMCC No.15466, and the address of the preservation unit is No. 3, No. 1 Yard, Beichen West Road, Chaoyang District, Beijing. The invention further provides an application of the lactobacillus plantarum CV10D1. The application comprises the restraining effect of the lactobacillus plantarum CV10D1 viable bacteria and fermentation liquor thereof to the growth of aspergillus and fusarium, and the effect of amixture of the lactobacillus plantarum CV10D1 fermentation liquor and chitosan to be used as an additive of pennisetum purpureum silage for jointly restraining the growth of rot fungi in the silage process of pennisetum purpureum, and more technical choices are provided for increasing the quality of the pennisetum purpureum silage through microorganisms.

The invention discloses a hybrid broussonetia papyrifera silage, which comprises the following raw materials: hybrid broussonetia papyrifera which is prepared into rubbed strips, molasses additive, lactic acid bacteria additive and cellulase additive, wherein the ratio of the raw materials in parts by weight: 100 parts of whole hybrid broussonetia papyrifera, 4 parts of molasses, 0.002 part of lactic acid bacteria and 0.1 part of cellulase. The broussonetia papyrifera is adopted as the raw material, the broussonetia papyrifera has the advantages of being fast in growth, high in adaptability, resistant to dry and cold, resistant to damp and heat, developed in root system, high in yield, resistant to cutting and the like, when the broussonetia papyrifera is used as the raw material of the silage, the raw material can not be affected by the environment easily, the quality of the produced silage is good, and due to the high fast-growing capacity, the yield of the produced silage is high. High moisture content of the broussonetia papyrifera raw material can cause nutrient loss and clostridium fermentation in the ensiling process, according to the invention, the moisture of the broussonetia papyriferais controlled to be 65% through airing before the broussonetia papyrifera is cut up and rubbed into strips, and the quality of the broussonetia papyrifera ensiling is guaranteed from the source.