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Microbial inoculum for preparing feed and application thereof

A technology of bacterial agent and feed, which is applied in the field of bacterial agent for preparing feed, can solve the problems of pollution and inhibition, narrow adaptability of growth conditions, high fermentation cost, etc., and achieves convenient transportation and use, long activity retention time, and obvious silage effect Effect

Inactive Publication Date: 2010-06-16
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the silage effect of these two types of bacterial agents is not very good, which may be caused by the following reasons: for a single bacterial agent, a single bacterium loses the opportunity to coexist with other bacteria, resulting in a greatly reduced ability to produce lactic acid. The adaptation range of growth conditions is narrow, the requirements for fermentation conditions are high, the fermentation cost is expensive, and a single bacterium grows in isolation, which is easily polluted and inhibited by other bacteria, making it difficult to become the dominant bacterium to control fermentation in silage; for simple mixed bacterial agents, It is just a simple mixture of several artificial bacteria, and there is no synergy between various bacteria, so the effect is not obvious

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1, the preparation of bacterial agent

[0020] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:

[0021] Composition of MRS medium:

[0022] Element

g / L

Glucose

20.0

Peptone

10.0

Beef Extract (Lab-Lemco’powder)

10.0

Yeast Extract

5.0

Sodium acetate (Sodium Acetate 3H 2 O)

5.0

Ammonium Citrate (Tri-ammonium Citrate)

2.0

K 2 HPO 4

2.0

MgSO 4 ·7H 2 o

0.58

MnSO 4 4H 2 o

0.25

Tween 80 (Tween 80)

1mL / L

[0023] Lactobacillus plantarum (Lactobacillus plantarum) was inoculated in MRS medium, cultured statically at 30°C for 1 day, and all the fermentation broth was collected as culture A;

[0024] Lactobacillus kimchii was inoculated in MRS medium, cultured statically at 30°C for 1 day, and all the fermentation broth was collected as culture B;

[0025] Lactobacillus farciminis was inoculate...

Embodiment 2

[0040] Embodiment 2, the preparation of bacterial agent

[0041] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:

[0042] Method As described in Example 1, culture A of Lactobacillus plantarum, culture B of Lactobacillus kimchii, culture C of Lactobacillus farciminis, and culture D of Lactobacillus pentosus were obtained respectively.

[0043] Mix the above four cultures A, B, C and D evenly to obtain a lactic acid bacteria complex culture, in which the cfu ratio of the four bacteria is Lactobacillus plantarum:Lactobacillus kimchii:Lactobacillus farciminis:Lactobacillus pentosus=50:15:15: 20.

[0044] 2. Preparation of liquid dosage form bacterial agent

[0045] Preparation of liquid carrier: Mix glycerin and EDTA to make a liquid adsorption carrier;

[0046] Mix the above-mentioned liquid adsorption carrier with the composite bacterial culture obtained in step 1, wherein the ratio of the active ingredient bacteria to glycerin and EDTA is: 1×10 11 CFU: 2...

Embodiment 3

[0057] Embodiment 3, the preparation of bacterial agent

[0058] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:

[0059] Method As described in Example 1, culture A of Lactobacillus plantarum, culture B of Lactobacillus kimchii, culture C of Lactobacillus farciminis, and culture D of Lactobacillus pentosus were obtained respectively.

[0060] Mix the above four cultures A, B, C and D evenly to obtain a lactic acid bacteria complex strain culture, wherein the cfu ratio of the four bacteria is Lactobacillus plantarum:Lactobacillus kimchii:Lactobacillus farciminis:Lactobacillus pentosus=55:20:20: 10.

[0061] 2. Preparation of solid dosage form bacterial agent

[0062] Using medical stone and straw powder as the adsorption carrier, mix the above-mentioned lactic acid bacteria composite bacterial strain culture with the adsorption carrier to make the bacteria adsorb on the carrier to obtain a solid dosage form of the lactic acid bacteria composite bacterial a...

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PUM

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Abstract

The invention discloses a microbial inoculum for preparing feed and application thereof. The active components of the microbial inoculum comprise Lactobacillus plantarum, Lactobacillus kimchii, Lactobacillus farciminis and Lactobacillus pentosus. The four inocula in the lactic acid bacteria composite system of the microbial inoculum of the invention have high synergetic effect, can rapidly reproduce, colonize and dominate in an unpasteurized natural raw material, effectively control microbiota in silage, rapidly generate a large amount of lactic acid, reduce the pH value of the feed to about 4.0, reduce the dry matter loss and the feed quality decline caused by growth of contaminated microbes in the silage period to the farthest extent, reduce the generation of ammonia nitrogen, and improve the fermentation quality of the silage. Compared with control, the lactic acid production amount and speed in the alfalfa silage taking the microbial inoculum of the invention as an additive are obviously increased, and the protein content, the dry matter content and the like of the obtained alfalfa feed are obviously improved.

Description

technical field [0001] The invention relates to a bacterial agent for preparing feed and its application. Background technique [0002] Alfalfa silage is an excellent forage grass, which has the characteristics of good palatability, high digestibility, long storage period, convenient preparation and easy mechanical operation. As a high-protein forage, alfalfa silage is used for feeding dairy cows, which can increase the milk production of dairy cows, and has a better effect than other forage silage. [0003] However, alfalfa silage is easily spoiled, which limits its application in actual production. The main cause of spoilage is that the alfalfa plant has high protein content, high buffer energy, low sugar content, and a large amount of air exists in the hollow stem tissue of alfalfa. [0004] The way to prevent spoilage of alfalfa silage is to add additives to it. The traditional silage additives in my country mainly include formic acid and formaldehyde, but these addit...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/14C12R1/225C12R1/25
Inventor 王小芬崔宗均胡跃高
Owner CHINA AGRI UNIV
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