Microbial inoculum for preparing feed and application thereof
A technology of bacterial agent and feed, which is applied in the field of bacterial agent for preparing feed, can solve the problems of pollution and inhibition, narrow adaptability of growth conditions, high fermentation cost, etc., and achieves convenient transportation and use, long activity retention time, and obvious silage effect Effect
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Embodiment 1
[0019] Embodiment 1, the preparation of bacterial agent
[0020] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:
[0021] Composition of MRS medium:
[0022] Element
g / L
Glucose
20.0
Peptone
10.0
Beef Extract (Lab-Lemco’powder)
10.0
Yeast Extract
5.0
Sodium acetate (Sodium Acetate 3H 2 O)
5.0
Ammonium Citrate (Tri-ammonium Citrate)
2.0
K 2 HPO 4
2.0
MgSO 4 ·7H 2 o
0.58
MnSO 4 4H 2 o
0.25
Tween 80 (Tween 80)
1mL / L
[0023] Lactobacillus plantarum (Lactobacillus plantarum) was inoculated in MRS medium, cultured statically at 30°C for 1 day, and all the fermentation broth was collected as culture A;
[0024] Lactobacillus kimchii was inoculated in MRS medium, cultured statically at 30°C for 1 day, and all the fermentation broth was collected as culture B;
[0025] Lactobacillus farciminis was inoculate...
Embodiment 2
[0040] Embodiment 2, the preparation of bacterial agent
[0041] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:
[0042] Method As described in Example 1, culture A of Lactobacillus plantarum, culture B of Lactobacillus kimchii, culture C of Lactobacillus farciminis, and culture D of Lactobacillus pentosus were obtained respectively.
[0043] Mix the above four cultures A, B, C and D evenly to obtain a lactic acid bacteria complex culture, in which the cfu ratio of the four bacteria is Lactobacillus plantarum:Lactobacillus kimchii:Lactobacillus farciminis:Lactobacillus pentosus=50:15:15: 20.
[0044] 2. Preparation of liquid dosage form bacterial agent
[0045] Preparation of liquid carrier: Mix glycerin and EDTA to make a liquid adsorption carrier;
[0046] Mix the above-mentioned liquid adsorption carrier with the composite bacterial culture obtained in step 1, wherein the ratio of the active ingredient bacteria to glycerin and EDTA is: 1×10 11 CFU: 2...
Embodiment 3
[0057] Embodiment 3, the preparation of bacterial agent
[0058] 1. Preparation of Lactic Acid Bacteria Composite Bacteria Culture:
[0059] Method As described in Example 1, culture A of Lactobacillus plantarum, culture B of Lactobacillus kimchii, culture C of Lactobacillus farciminis, and culture D of Lactobacillus pentosus were obtained respectively.
[0060] Mix the above four cultures A, B, C and D evenly to obtain a lactic acid bacteria complex strain culture, wherein the cfu ratio of the four bacteria is Lactobacillus plantarum:Lactobacillus kimchii:Lactobacillus farciminis:Lactobacillus pentosus=55:20:20: 10.
[0061] 2. Preparation of solid dosage form bacterial agent
[0062] Using medical stone and straw powder as the adsorption carrier, mix the above-mentioned lactic acid bacteria composite bacterial strain culture with the adsorption carrier to make the bacteria adsorb on the carrier to obtain a solid dosage form of the lactic acid bacteria composite bacterial a...
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