Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of immune cell cryopreservation liquid and cryopreservation method

A technology of immune cells and cryopreservation, applied in the field of cell biology, can solve the problems of cell cryopreservation toxicity easily causing allergic reactions, inability to clinically reinfuse, etc., to improve cryopreservation effect, high clinical safety, and simple formula Effect

Active Publication Date: 2021-06-01
重庆斯德姆生物技术有限公司
View PDF10 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides an immune cell cryopreservation solution and a cryopreservation method, which can effectively solve the problem that the cell cryopreservation solution in the prior art has certain toxicity and is easy to cause allergic reactions, and cannot be directly used in clinical practice. Return problem

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] An immune cell cryopreservation solution, including a basal medium and supplementary components, wherein the basal medium is a DMEM / F12 medium, and the supplementary components include the following final concentrations of components: trehalose 1g / mL, propylene glycol 3v / v%, Acetamide 3v / v%, dextran 5v / v%, hydroxyethyl starch 1g / mL, glucose 0.5g / mL, heparin sodium 50U / mL, pearl grass extract 5mg / mL and BCG complex polysaccharide 0.5mg / mL .

[0024] Among them, the pearl grass extract is obtained as follows: take the pearl grass, clean it, dry it, grind it to 100 mesh, mix the powder with absolute ethanol at a weight ratio of 1:1, and then carry out CO 2 Supercritical extraction, finally centrifuged to remove impurities, to obtain a fat-soluble extract, add 6 times the weight of distilled water to the extraction residue, heat at 90°C for 8 hours, then filter, concentrate, and finally vacuum freeze-dry to obtain a water-soluble extract, combine the two The second extract...

Embodiment 2

[0027] An immune cell cryopreservation solution, including a basal medium and supplementary components, wherein the basal medium is a DMEM / F12 medium, and the supplementary components include components with the following final concentrations: trehalose 5g / mL, propylene glycol 6v / v%, Acetamide 5v / v%, dextran 10v / v%, hydroxyethyl starch 3g / mL, glucose 1.5g / mL, heparin sodium 150U / mL, pearl grass extract 15mg / mL and BCG complex polysaccharide 1.5mg / mL .

[0028] Among them, the pearl grass extract is obtained in this way: take the pearl grass, clean it, dry it, grind it to 100 mesh, mix the powder with absolute ethanol at a weight ratio of 3:1, and then carry out CO 2 Supercritical extraction, finally centrifuged to remove impurities, to obtain a fat-soluble extract, add 8 times the weight of distilled water to the extraction residue, heat at 95°C for 8h, then filter, concentrate, and finally vacuum freeze-dry to obtain a water-soluble extract, combine the two The second extrac...

Embodiment 3

[0031] An immune cell cryopreservation solution, including a basal medium and supplementary components, wherein the basal medium is a DMEM / F12 medium, and the supplementary components include components with the following final concentrations: trehalose 3g / mL, propylene glycol 3-6v / v %, acetamide 4v / v%, dextran 8v / v%, hydroxyethyl starch 2g / mL, glucose 1.0g / mL, heparin sodium 100U / mL, pearl grass extract 10mg / mL and BCG complex polysaccharide 0.8mg / mL.

[0032] Among them, the pearl grass extract is obtained in this way: take the pearl grass, wash it, dry it, grind it to 100 mesh, mix the powder with absolute ethanol at a weight ratio of 2:1, and then carry out CO 2 Supercritical extraction, finally centrifuged to remove impurities, to obtain a fat-soluble extract, add 8 times the weight of distilled water to the extraction residue, heat at 90°C for 8 hours, then filter, concentrate, and finally vacuum freeze-dry to obtain a water-soluble extract, combine the two The second ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an immune cell cryopreservation solution and a cryopreservation method. The cryopreservation solution includes a basal medium and additional components, wherein the additional components include components with the following final concentrations: trehalose 1-5g / mL, propylene glycol 3 ‑6v / v%, acetamide 3‑5v / v%, dextran 5‑10v / v%, hydroxyethyl starch 1‑3g / mL, glucose 0.5‑1.5g / mL, heparin sodium 50‑150U / mL, pearl Grass extract 5-15mg / mL and BCG complex polysaccharide 0.5-1.5mg / mL. The formula of the cryopreservation liquid of the present invention is simple, and each component cooperates with each other and acts synergistically. Under the cryopreservation method provided, the cryopreservation effect of endothelial progenitor cells can be significantly improved, and at the same time, it can also ensure that the cell proliferation activity after recovery is not affected, ensuring that the cell Intracellular water does not crystallize near freezing point.

Description

technical field [0001] The invention belongs to the technical field of cell biology, and in particular relates to an immune cell cryopreservation solution and a cryopreservation method. Background technique [0002] Immune cell therapy is a new type of autoimmune anti-cancer treatment. It uses biotechnology and biological agents to culture and amplify immune cells collected from patients in vitro and then reinfuse them back into patients to stimulate and enhance immunity. The body's own immune function, so as to achieve the purpose of treating tumors. [0003] With the rapid development of immune cell biology and immune molecular biology, somatic cell immunotherapy has become one of the important means of adjuvant therapy after radiotherapy and chemotherapy for tumor patients. All have good effects. [0004] However, the effect of in vitro culture of immune cells depends largely on the number and quality of immune cells in the patient, but the number of immune cells in tum...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 熊华强秦连荣熊荣骞
Owner 重庆斯德姆生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com