61results about How to "Produce toxicity" patented technology

The invention provides a hydrogel dressing which comprises HEC (hydroxyethyl cellulose), propylene glycol, water and one of CMCS (carboxymethyl chitosan) and chitosan hyamine. A preparation process of the hydrogel dressing includes the steps: taking components for standby application according to the ratio of the components as demanded; dissolving the CMCS or the chitosan hyamine in water and heating the CMCS or the chitosan hyamine; adding the HEC into solution and sufficiently stirring the solution; adding the propylene glycol into the solution and sufficiently stirring the solution to obtain final solution; and standing and defoaming the final solution to obtain the hydrogel dressing. The hydrogel dressing has fine biocompatibility and degradability, and has a sterilizing function, so that high antibacterial activity can be achieved when a wound is treated, and high toxicity is avoided.

The invention discloses a making method of cellulose aquagel, which is characterized by the following: dissolving cellulose into ionic liquid; replacing ionic liquid; obtaining the white semi-transparent product.

The invention provides application of a DNA (Deoxyribonucleic Acid) tetrahedron to preparation of a medicament for promoting neural restoration. The DNA tetrahedron is assembled into a tetrahedron structure from four single-chain DNAs in a complementary base pairing manner; each edge of the tetrahedron is of a dual-chain DNA structure. The DNA tetrahedron provided by the invention can remarkably promote the proliferation, differentiation and migration of mouse neural stem cell, and has high biocompatibility and bioavailability.

The invention discloses nitrogen-doped carbon aerogel prepared by utilizing natural-structure macromolecular nano-fiber aerogel. A biomass polysaccharide structural macromolecular material is prepared into cellulose aerogel through a mechanical method, a chemical oxidization pre-treatment method and an enzyme pre-treatment method, and is then treated in a nitrogen-rich atmosphere by a high-temperature pyrolysis method. With the adoption of the nitrogen-doped carbon aerogel, the utilization value of the biomass polysaccharide structural macromolecular material is improved, and novel concept and method are provided for efficiently utilizing carbon material resources.

The invention provides a wood waterproof agent adopting a silicone emulsion, a method for preparing the wood waterproof agent, and a method for preparing waterproof wood by utilizing the wood waterproof agent. The method for preparing the wood waterproof agent comprises the following steps: first, preparing a silicone oil emulsion primary liquid which contains hydroxyl silicone oil, hydrogen-containing silicone oil, a surfactant, nano-silicon dioxide, micron-silicon dioxide and water; then, diluting the silicone oil emulsion primary liquid into silicone oil emulsions of different concentrations; and finally, adding a catalyst for uniform dissolution and mixing. When the silicone oil emulsion wood waterproof agent provided by the invention is utilized for vacuum-pressurization pretreatment of wood, the waterproof performance and size stability of the wood can be improved, the hygroscopicity of the wood can be reduced, and the contact angle between the treated wood surface and water is larger than 140-150.

The invention discloses a reinforcing method utilizing waterlogged wood relics of nanocellulose. The reinforcing method comprises the following steps of: mixing nanocellulose with a filling agent to obtain a reinforcing agent; and performing dehydrating and desalination on the waterlogged wood relics, and adding the waterlogged wood relics into the reinforcing agent to soak, and performing drying for reinforcing. The waterlogged wood relics obtained by treatment of the reinforcing method are bright in color, are good in stability, do not easily separate out salts, and are especially free of secondary acid degradation. The reinforcing method disclosed by the invention can be used for effectively clearing and protecting the reinforcing method, the dried and reinforced wood is stabilized to a pH neutral state, acid corrosion and salt separation are effectively restrained, and mechanical strength of highly rotten wood is greatly reinforced.

The invention discloses a chitin-nanometer-fiber aerogel with the high specific surface area. A preparing method includes the steps that a chitin nanometer fiber is prepared with the TEMPO oxidizing method or the mechanical method, and then the superfine nanometer-chitin aerogel is prepared through higher alcohol replacement and drying. According to the chitin-nanometer-fiber aerogel and the preparing method, the preparing process is simple, and the technology is easy to control; a used oxidizing agent is an environment-friendly solvent, safe and nontoxic, the environment-friendly preparing technology is achieved, and cost is reduced; the prepared chitin-nanometer-fiber aerogel is nontoxic, low in density, high in porosity and even in structural unit distribution, and most prominently, the high specific surface area is achieved.

The invention relates to a platelet membrane wrapped drug loading porous nano particle and a preparation method of the nano particle. The nano particle is prepared by modifying PLGA (poly (lactic-co-glycolic acid)) with chitosan oligosaccharide CS, loading Bufotalin and then wrapping with a platelet membrane. The preparation method comprises the steps of preparation of chitosan oligosaccharide modified PLGA, preparation of CS-pPLGA / Bu NPs, preparation of a platelet membrane fragment and preparation of PLTM-CS-pPLGA / Bu NPs. According to the method, the nano particle with the positively chargedsurface is prepared from CS modified PLGA; absorption of the negatively charged platelet membrane is facilitated by a layer-by-layer self-assembly manner; the stability of the nano particle is improved; and the prepared platelet membrane bionic coated porous nano particle is expected to achieve excellent biocompatibility, low immunogenicity and active cancer cell targeting ability, can actively deliver chemotherapeutics to tumor cells in a targeted manner, improves a treatment effect and has a very good practical value.

The invention discloses chitin nano-fibril. The chitin nano-fibril is characterized in that the diameter is smaller than or equal to 10 nm, and the chitin nano-fibril is prepared with a TEMPO oxidation method. The invention further discloses specific steps. The preparation process is simple, the technology is easy to control, a used oxidant is an environment-friendly solvent and is safe and non-toxic, an environment-friendly preparation process is realized, the cost is reduced, and the prepared chitin nano-fibril is non-toxic, has a high length-diameter ratio, is uniform in structure unit distribution and has certain mechanical strength.

The invention discloses an immune cell cryopreservation liquid and a cryopreservation method. The cryopreservation liquid comprises a basic culture medium and additives. The additives include, by final concentration, 1-5 g / mL of trehalose, 3-6 v / v% of propylene glycol, 3-5 v / v% of acetamide, 5-10 v / v% of dextran, 1-3 g / mL of hydroxyethyl starch, 0.5-1.5 g / mL of glucose, 50-150 U / mL of heparin sodium, 5-15 mg / mL of herba phyllanthi urinariae extract and 0.5-1.5 mg / mL of bacillus Calmette-Guerin composite polysaccharides. The cryopreservation liquid is simple in formulation, interaction and synergistic effects of the components are achieved, endothelial progenitor cell cryopreservation effects can be evidently improved by the adoption of the cryopreservation method, insusceptibility of post-reviving cell proliferation activity can be guaranteed, and it is guaranteed that intracellular moisture of cells close to a freezing point is free of crystallization.

The invention discloses carbon aerogel prepared from chitin aerogel. Chitin nano-fiber aerogel is prepared by means of a mechanical method or a chemical oxidation method, and then the carbon aerogel is obtained through a method for carbonization under the inert gas shielding. The preparation method is simple, the technology is easy to control, the adopted oxidizing agent is the environment-friendly green solvent and is safe and free of toxicity, the green preparation technology is achieved, the cost is lowered, the obtained carbon aerogel is free of toxicity, the 3D network structure is obtained, the surface area is large, the porosity is high, and the certain mechanical strength is achieved.

The invention provides a preparation method of a lignocellulose / silver halide composite material and the lignocellulose / silver halide composite material prepared through the method.In the method, only lignocellulose, ionic liquid and silver-containing compounds are adopted for synthesis through microwave heating, and the lignocellulose / silver halide composite material with excellent photocatalytic activity is obtained.The preparation method is simple in technology and easy to implement, no complicated and expensive equipment is needed, and industrial popularization is facilitated.

The invention discloses application of an asymmetrical 1,2,4,5-tetrazine activating agent to a prodrug or rapid activation of protein in the bioorthogonal field based on an inverse electron Diels-Alder reaction. The application disclosed by the invention has the advantages that compared with a symmetrical tetrazine molecule, the asymmetrical 1,2,4,5-tetrazine activating agent has the advantages of extremely high activating efficiency and no toxicity to cells in the asymmetrical 1,2,4,5-tetrazine activating agent and the bioorthogonal reaction process, can be used for rapidly activating the prodrug, in particular to proteins taking lysine, tyrosine, serine or threonine as active residues in vitro or vivo, can be used for the aspects such as research on life course and treatment on diseases, and has an extremely wide application prospect.

The invention relates to a preparation method of a bacterial cellulose solution, which comprises the following steps: (1) mixing N-methylimidazole with allyl chloride, and treating the mixture by rotary evaporation to obtain <AMIM>Cl; or mixing N-methylimidazole with 1-chlorobutane, and treating the mixture by rotary evaporation to obtain <BMIM>Cl; and (2) mixing the bacterial cellulose with the ionic liquid, and heating and stirring the mixture at 30-100 DEG C in a water bath. The invention has simple preparation processes, has no need of pretreatment of the cellulose materials, such as derivation, activation and the like, has less energy consumption and is suitable for industrial production.

The invention belongs to the technical field of biology, in particular to a diagnostic nano-drug which utilizes a photothermal effect to assist deep penetration of tumors. A drug-probe coupling body is synthesized by a one-pot method, and a probe is in a quenched state in the coupled state; targeted biodegradable polylactic acid-glycolic acid copolymer (PEG-PLGA) micelles can be used for targeteddelivery to a tumor site. However, under the stimulation of high-concentration glutathione in tumor microenvironment, drugs and probe molecules can be released simultaneously, in the same place and inthe same amount, quenching probe molecules can emit near-infrared fluorescence when activated to achieve the purpose of real-time reporting of drug release, the in-situ released probes can generate aphotothermal effect under near-infrared illumination to increase the temperature of a tumor region, increase blood flow and blood flow volume, and meanwhile, molecular diffusion is increased to achieve the purpose of deep penetration into the tumor region, and finally, triple purposes of tracing, targeting and photothermal penetration are achieved.

The invention relates to an erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof. The erythrocyte membrane coated polydopamine coated drug-loaded PLGA material comprises an erythrocyte membrane coated polydopamine coated anticancer drug-loaded PLGA nano-carrier RBCM-PDA-CUR@PLGA. The adopted erythrocyte membrane, PLGA, TPGS and DAare biological materials with very good biocompatibility, and do not generate toxicity to the body. The preparation method is simple to operate and mild in reaction conditions.

The invention provides a drug sustained-release system for treating tumours, which comprises a drug carrier, a drug and a surface active agent. The drug carrier is a biodegradable polymer fibre. The drug is a dichloroacetic acid salt covered and loaded on the drug carrier. The surface active agent is covered and loaded on the drug carrier, wherein the drug accounts for 15-70 wt % of the drug sustained-release system and the surface active agent accounts for 0-15 wt % of the drug sustained-release system. The invention further provides a preparation method of the drug sustained-release system for treating the tumours. The drug sustained-release system can realize the sustained release of the local drug so that the concentration of the local drug around the tumours is in a scope of a therapeutic effect window; however, the drug concentration except the tumours and in body fluid is in a health scope, therefore, the utilization rate of the drugs is increased and the therapeutic effect is guaranteed as well as the usage amount of the drugs is reduced and the toxic side effects caused by large dosage of the drugs are reduced.

The invention discloses a paraffin emulsion-borate compound modifier which comprises the following components in parts by weight: 1-2000 parts of solid paraffin of which the melting point is 48-64 DEG C, 1-500 parts of a nonionic surfactant, 1-200 parts of an anion surfactant, 1-200 parts of a cosurfactant, 1-200 parts of fatty acid, 1-2000 parts of borate and 1-10,000 parts of water. The paraffin emulsion-borate compound modifier disclosed by the invention is utilized to perform pretreatment on high-heat treatment material so as to improve the mechanical property, the dimensional stability and the property of preventing termites of the high-heat treatment material.

The invention relates to a method for preparing injection level bio-based plastic from ferric sodium tartrate and glycerol-shielded wood powder hydroxyl, which belongs to the field of bio-based plastic development. The purpose is to provide the bio-based plastic preparation method which is simple in process and low in production cost and can easily realize industrialization. Wood fiber material is ground, a ball mill is then used for ball-milling the material, so that powder is obtained, and the ball milling time is 4 to 15 hours. The wood fiber powder preprocessed by ball milling is added into a vessel in which ferric sodium tartrate solution is prepared, the mixture is swelled under a low temperature of 1 DEG C to 5 DEG C in a refrigerator, then keanded in a kneader at a cavity temperature of 30 DEG C to 80 DEG C for 3 to 6 hours and dried, so that an initial sample with certain thermoplasticity is obtained, glycerol, the part by weight of which is 5 to 40 percent, is added into the initial sample, and the initial sample is circulated in a double-screw extruder under the conditions of 50 DEG C to 140 DEG C in temperature and 30r / min to 110r / min in rotational speed for 5 to 60 minutes, and is then extruded out, so that the injection level bio-based plastic is obtained. The process of the method is simple, the resource utilization rate is high, and the obtained product has the advantages of high mechanical property, environment-friendliness, bio-degradability and the like.

The invention relates to a Mn<2+>-doped polydopamine nano-carrier and a preparation method thereof. The Mn<2+>-doped polydopamine nano-carrier is obtained by polymerizing dopamine on the surface of PLGA NPs and doping Mn<2+>. The nano-carrier is gathered at a tumor part through an EPR effect, and hydroxyl radicals are generated through a Fenton-like reaction initiated by the Mn<2+>. A foundation is laid for a nano-system for realizing tumor microenvironment response and biomimetic material polydopamine long-acting circulation.

The invention belongs to the technical field of pharmaceutical preparations, and particularly relates to a bioadhesive type hollow microsphere of two alkalescent medicaments: dipyridamole and famotidine, and a preparation method thereof. The bioadhesive type hollow microsphere comprises medicaments, a skeleton material, a bioadhesive material, an emulsifying agent and a dispersing agent, wherein the preferred ratio of the medicaments to the skeleton material is 1 to (4-6). The bioadhesive type hollow microsphere is prepared by adopting an O / O emulsifying agent dispersion-volatilization method including the following steps: mixing the medicaments, the skeleton material and the bioadhesive type material weighed according to the amount of the prescription into an organic solvent, and stirring under low-temperature water bath condition for dissolving the medicaments, the skeleton material and the bioadhesive type material to obtain a dispersion phase; under the condition of 10DEG C water bath mechanical stirring, slowly dropwise adding the dispersion phase into liquid paraffin containing the emulsifying agent and the dispersing agent, performing low-temperature emulsification, heating and volatilizing to remove the organic solvent, separating, washing with n-hexane, drying and screening to obtain the bioadhesive type hollow microsphere. The prepared microsphere is high in encapsulation efficiency and yield, long in in-vivo retention time, and good in floatability, bioadhesion and slow-release property.

The invention discloses a multifunctional targeted ultrasound contrast agent. The contrast agent comprises carboxyl-terminated polylactic acid, wherein the carboxyl-terminated polylactic acid is filled with fluorocarbon gas to form carboxyl-terminated polylactic acid microspheres, the carboxyl-terminated polylactic acid loads Sudan black dye, and the carboxyl terminal of the carboxyl-terminated polylactic acid is coupled with an anti-human VEGFR-3 monoclonal antibody through a covalent bond. The contrast agent can more accurately and effectively perform positioning and be firmly combined on the surface of lymphatic endothelial cells, not only has strong target searching capability, but also has ultrasonic contrast imaging and intraoperative indication function, also has the function of preventing tumor metastasis, and is a multifunctional ultrasonic contrast agent with both blue staining trace effect and ultrasonic imaging effect and treatment. The invention also discloses a preparation method of the contrast agent, and the method utilizes a double emulsification method to prepare the ultrasonic contrast agent with the blue staining indication function, and uses EDC / NHS as a coupling activator, and covalently connects VEGFR-3 on the surface of the ultrasonic contrast agent. The method is simple and easy to operate, and is suitable for large-scale production.

The invention discloses a method for preparing ZnO nanopowder from a cellulose zinc chloride solution. The method includes that with a high-concentration ZnCl2 solution as a cellulose solvent, a nano-zinc oxide as a zinc source and cellulose dissolved in ZnCl2 as both a ZnO nanopowder preparation process control auxiliary and a macromolecular blocking agent for ZnO nanopowder agglomeration, the agglomeration-free ZnO nanopowder uniform in size is prepared in a colloid grinder serving as an efficient mixing reactor at a low temperature. The method for preparing the ZnO nanopowder from the cellulose zinc chloride solution has the advantages that generation of ZnO nanoparticles under high-concentration reactants at the low temperature is effectively promoted under the action of a great number of hydroxyls and zinc ions on dissolved cellulose molecules as well as efficient mixing of the colloid grinder, and accordingly the method is high in reactant concentration, simple to implement, low in energy consumption and easy for industrial production.

A bactericide for agricultural and horticultural use containing an oxime ether derivative represented by formula (I) or at least one salt thereof. (In formula (I), X represents a halogen atom, a C1-20 alkyl group or the like; R1 and R2 each represents a hydrogen atom, a C1-20 alkyl group or the like; R3 represents a hydrogen atom, a C1-20 alkyl group or the like; R4 represents a hydrogen atom, a C1-20 alkyl group or the like; R5 represents a hydrogen atom or the like; Y represents an oxygen atom or the like; Z represents an oxygen atom or the like; Q represents an aryl group or the like; m represents an integer of 0-8; and n represents an integer of 0-4).

The invention discloses a special large molecular gel with environment sensitivity. According to a preparation method, metalloprotein is embedded into a gel three dimensional network through chemicalcovalent bonds. The gel is prepared through copolymerization of an environment sensitive gel monomer or a skeleton formed through crosslinking of the environment sensitive gel monomer with a metalloprotein containing reaction groups or a complex of the metalloprotein with metal ions. Shrinkage and swelling of the environment sensitive gel with changing of environment can be realized, so that the chelating capacity of the metalloprotein on metal ions is adjusted, and high efficiency and selective capturing and release of metal ions are realized. The special large molecular gel with environmentsensitivity is capable of realizing high efficiency, stable, circulation capturing of metal ions at extremely concentration in liquid.

The invention discloses a separation and culture method of umbilical cord blood derived mesenchymal stem cells, and belongs to the technical field of biology. The separation and culture method comprises the following steps of performing centrifuging treatment on umbilical cord blood, taking an upper layer to obtain plasma, and taking a lower layer to obtain cells; performing inactivation treatmenton the plasma, then performing centrifuging treatment, and taking supernatant so as to obtain autoserum; mixing the lower-layer cells with normal saline to obtain a mixture, then placing the mixturein lymphocyte separation liquid, and performing centrifuging treatment so as to obtain umbilical cord blood mononuclear cells; and re-suspending the umbilical cord blood mononuclear cells with serum-free culture mediums, placing the re-suspended umbilical cord blood mononuclear cells in an autoserum coating culture dish, performing primary culture to obtain primary mesenchymal stem cells, adding cell dissociation liquid into the primary mesenchymal stem cells, performing cell dissociation treatment, and then performing subculturing. According to the method, penicllin-streptomycin for cell culture is not added in a separation process and a culture process, so that toxicity is not generated on the cells. In addition, according to the method, the autoserum coating culture dish for centrifugalseparation is adopted, so that animal derived viruses cannot be introduced.

The invention discloses a humic acid urea, a preparation method thereof, and a composition for preparing the humic acid urea, and relates to the fields of fertilizers and chemical industry. The composition comprises, by weight, 60-150 parts of first urea, 17-35 parts of humic acid powder, and 3.5-12 parts of a composite binder. Raw materials of the composite binder include, by weight, 1.5-2.5 parts of second urea, 5-30 parts of humate, 3-15 parts of clay and 65-85 parts of water. The preparation method comprises the following steps: stirring and mixing the first urea and the composite binder for 2-6 min, adding the humic acid powder at 20-45 DEG C, and performing stirring for 5-10 min. The humic acid urea is prepared from the composition. The humic acid urea can effectively improve the utilization rate of nitrogen, and also can avoid environmental pollution. The preparation method has the advantages of simplicity, convenience, low cost, convenience in operation enforcement, and suitableness for large-scale industrial production. The composition has the advantages of easiness in obtaining, low cost, and great reduction of the requirements of raw materials.

The invention discloses carbon aerogel prepared from chitin aerogel. Chitin nano-fiber aerogel is prepared by means of a mechanical method or a chemical oxidation method, and then the carbon aerogel is obtained through a method for carbonization under the inert gas shielding. The preparation method is simple, the technology is easy to control, the adopted oxidizing agent is the environment-friendly green solvent and is safe and free of toxicity, the green preparation technology is achieved, the cost is lowered, the obtained carbon aerogel is free of toxicity, the 3D network structure is obtained, the surface area is large, the porosity is high, and the certain mechanical strength is achieved.