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Erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof

A technology of dopamine and drug loading, which is applied in the direction of wave energy or particle radiation treatment materials, drug combinations, antineoplastic drugs, etc., to achieve the effects of increasing drug release, reducing rejection, and inhibiting growth

Active Publication Date: 2020-03-24
河北英治医疗器械科研有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] CN108703959A discloses a PLGA nano-carrier of red blood cell wrapped anticancer drugs and its preparation and application, but it is only limited to the means of chemotherapy

Method used

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  • Erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof
  • Erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof
  • Erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Dissolve 10 mg PLGA, 1 mg CUR and 2 mg TPGS in 1 mL of acetone, sonicate until dissolved, and then slowly add to a round bottom flask containing 5 mL of deionized water at a rotation speed of 1000 rpm. Stir for 3 hours, put into a vacuum drying oven to volatilize acetone, centrifuge at 8000rpm for 10min, and resuspend with 3mL Tris-buffer to obtain drug-loaded nanocarriers.

[0042] (2) 5 mg DA was dissolved in 2 mL of Tris-buffer, and added dropwise to the drug-loaded nanocarrier in step (1). Stir at 800 rpm at 25°C for 6 hours, centrifuge and wash three times at 11000 rpm, and resuspend with 5 mL of deionized water to obtain a PDA-coated drug-loaded nanocarrier.

[0043] (3) Blood was collected from the heart of SD rats, the blood was centrifuged at 3000 rpm for 5 min, and then washed three times with phosphate buffered saline (PBS, pH=7.4) to remove serum. Red blood cells were resuspended in 0.2 mM EDTA solution to induce membrane rupture. The supernatant was rem...

Embodiment 2

[0047] According to the method of Example 1, unloaded PLGA nanocarriers, PDA-coated unloaded PLGA nanocarriers, PDA-coated PLGA drug-loaded nanocarriers, and erythrocyte membrane-encapsulated PDA-coated PLGA drug-loaded nanocarriers were prepared. Deionized water was used to prepare a solution with a concentration of 500 μg / mL, and then the hydrodynamic particle size was measured at 25 °C.

[0048] The results of measuring the hydrodynamic particle size of the four nanocarriers at 25 °C are as follows: figure 2 As shown, the particle size of blank PLGA nanocarriers is 164.2±2 nm; the particle size of blank PDA-coated PLGA nanocarriers is 190.2±3 nm; the particle size of PDA-coated PLGA drug-loaded nanocarriers is 190.5±3 nm; PDA coated with red blood cell membrane The particle size of PLGA drug-loaded nanocarriers is 220.2±4nm. The results showed that the particle size of the PDA-coated PLGA drug-loaded nanocarriers wrapped by the erythrocyte membrane became larger, which pr...

Embodiment 3

[0050] Red blood cell membranes, unloaded PLGA nanocarriers, PDA-coated unloaded PLGA nanocarriers, PDA-coated PLGA drug-loaded nanocarriers, and erythrocyte membrane-wrapped PDA-coated PLGA drug-loaded nanocarriers were prepared according to the method of Example 1 . Deionized water was used to prepare a solution with a concentration of 500 μg / mL, and then the Zeta potential was measured at 25 °C.

[0051] Five carrier Zeta potentials such as image 3 As shown, the Zeta potential of RBCM is -19.2±2mv; the Zeta potential of blank PLGA nanocarriers is -14.8±2mv; the Zeta potential of blank PDA-coated PLGA nanocarriers is -25.3±1mv; PDA-coated PLGA drug-loaded nanocarriers The Zeta potential of erythrocyte membrane-wrapped PDA-coated PLGA drug-loaded nanocarriers was -28.8±2mv. The results showed that the Zeta potential of the erythrocyte membrane encapsulated became larger, which proved the successful encapsulation of the erythrocyte membrane.

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Abstract

The invention relates to an erythrocyte membrane coated polydopamine coated drug-loaded PLGA material as well as preparation and application thereof. The erythrocyte membrane coated polydopamine coated drug-loaded PLGA material comprises an erythrocyte membrane coated polydopamine coated anticancer drug-loaded PLGA nano-carrier RBCM-PDA-CUR@PLGA. The adopted erythrocyte membrane, PLGA, TPGS and DAare biological materials with very good biocompatibility, and do not generate toxicity to the body. The preparation method is simple to operate and mild in reaction conditions.

Description

technical field [0001] The invention belongs to the field of chemotherapeutic and photothermal integrated medicine and its preparation and application, in particular to a erythrocyte membrane-wrapped polydopamine-coated drug-loaded PLGA material and its preparation and application. Background technique [0002] Erythrocytes are a natural long-term circulating carrier. The membrane protein CD47 on its surface can regulate the uptake of macrophages through the interaction with the CD47 receptor-signal regulatory protein on the surface of macrophages, so that nanocarriers can Evade the immune recognition in the body, gather at the tumor site through the EPR effect, and realize the inhibitory effect on cancer cells. Red blood cell membranes (RBCMs) have been widely used for drug delivery and other therapeutic applications. [0003] Polydopamine (PDA) is a biopolymer polymerized from dopamine (DA), which has excellent biocompatibility and low cytotoxicity. In terms of optical p...

Claims

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Application Information

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IPC IPC(8): A61K9/50A61K41/00A61K31/12A61K47/34A61K47/46A61P35/00
CPCA61K9/5068A61K9/5073A61K9/5089A61K31/12A61K41/0052A61K47/34A61P35/00A61K2300/00
Inventor 朱利民谢晓田王海军吴梦
Owner 河北英治医疗器械科研有限公司
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