Method for improving encapsulation-dehydration ultralow-temperature preservation effect of early pear stem tips
A technology of ultra-low temperature preservation and dehydration method, which is applied in the preservation of plants, botanical equipment and methods, and applications, and can solve the problems of low survival rate and poor preservation effect.
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[0004] The present invention will be further described in conjunction with following embodiment:
[0005] (1) First processing
[0006] In the ultra-clean workbench, cut the stem tip of early pear and inoculate it in liquid medium one: MS+0.3-0.5g / L carbon nanotube+0.3-0.5g / L graphene quantum dot+3% (w / v) sodium alginate+0.3M sucrose;
[0007] (2) The second treatment
[0008] Transfer the shoot tip to the liquid medium two: 0.4M sucrose + 0.3-0.5g / L carbon nanotubes + 0.3-0.5g / L graphene quantum dots + 0.1M CaCl 2 , for conventional embedding;
[0009] (3) The third treatment
[0010] Transfer the embedding beads to liquid medium three: MS+0.3-0.5g / L carbon nanotubes+0.3-0.5g / L graphene quantum dots+0.5M sucrose for conventional pre-cultivation;
[0011] (4) The fourth treatment
[0012] After routine air or silica gel dehydration, liquid nitrogen routine storage, and routine thawing, transfer the embedded beads to solid regeneration medium IV: MS+1-2mg / L Thidiazuron+0...
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