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Method for improving encapsulation-dehydration ultralow-temperature preservation effect of early pear stem tips

A technology of ultra-low temperature preservation and dehydration method, which is applied in the preservation of plants, botanical equipment and methods, and applications, and can solve the problems of low survival rate and poor preservation effect.

Active Publication Date: 2018-11-02
SHANGRAO NORMAL UNIV
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  • Summary
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AI Technical Summary

Problems solved by technology

However, the cryopreservation method of embedding and dehydration also has problems such as low survival rate and poor preservation effect.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0004] The present invention will be further described in conjunction with following embodiment:

[0005] (1) First processing

[0006] In the ultra-clean workbench, cut the stem tip of early pear and inoculate it in liquid medium one: MS+0.3-0.5g / L carbon nanotube+0.3-0.5g / L graphene quantum dot+3% (w / v) sodium alginate+0.3M sucrose;

[0007] (2) The second treatment

[0008] Transfer the shoot tip to the liquid medium two: 0.4M sucrose + 0.3-0.5g / L carbon nanotubes + 0.3-0.5g / L graphene quantum dots + 0.1M CaCl 2 , for conventional embedding;

[0009] (3) The third treatment

[0010] Transfer the embedding beads to liquid medium three: MS+0.3-0.5g / L carbon nanotubes+0.3-0.5g / L graphene quantum dots+0.5M sucrose for conventional pre-cultivation;

[0011] (4) The fourth treatment

[0012] After routine air or silica gel dehydration, liquid nitrogen routine storage, and routine thawing, transfer the embedded beads to solid regeneration medium IV: MS+1-2mg / L Thidiazuron+0...

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Abstract

The invention provides a method for improving an encapsulation-dehydration ultralow-temperature preservation effect of early pear stem tips. The early pear stem tips are treated in sequence by utilizing four types of culture media containing carbon nanotubes and graphene quantum dots, and the survival rate of the frozen stem tips can be remarkably improved. Compared with the prior art, the methodhas the advantages that the encapsulation-dehydration ultralow-temperature preservation effect of the early pear stem tips is good, the survival rate is high and the stem tips are easy to regenerate.

Description

Technical field: [0001] The invention relates to a method for improving the ultra-low temperature preservation effect of plant stem tip embedding and dehydration method, specifically a method for improving the ultra-low temperature preservation effect of early pear stem tip embedding and dehydration method. Background technique: [0002] my country has a long history of pear tree planting, and it has a strong ability to adapt to the environment. At present, there are many local premium varieties adapted to the local environment in the north and south of the Yangtze River. Early-maturing pear tree varieties are commonly known as southern early-maturing pears. Their pears basically mature between June and August, which is significantly earlier than northern pears. It fills the shortage of fresh fruit supply in summer and is welcomed and loved by the general public. Huating Town and Tiandun Town, Shangrao County, Jiangxi Province have a pleasant climate, abundant rainfall, and ...

Claims

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Application Information

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IPC IPC(8): A01N3/00
CPCA01N3/00
Inventor 尹明华洪森荣郁雪婷
Owner SHANGRAO NORMAL UNIV
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