Immune cell cryopreservation solution and immune cell cryopreservation method
A technology of immune cells and cryopreservation method, applied in the field of immune cell cryopreservation, to achieve the effect of simple formula, high clinical safety, and unaffected activity
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Embodiment 1
[0044]PBMC cryopreservation and recovery of peripheral blood mononuclear cells:
[0045] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:
[0046] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:
[0047] A freezing medium:
[0048] Human Albumin 10ml
[0049] Cell Stabilizer 3ml
[0050] KBM581 serum-free medium 87ml
[0051] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0052] B cryopreservation solution:
[0053] Medical DMSO 20ml
[0054] KBM581 serum-free medium 80ml
[0055] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0056] Use the above prepared immune freezing medium to freeze peripheral blood mononuclear cells PBMC:
[0057] 1. Take huma...
Embodiment 2
[0063] Cryopreservation and recovery of umbilical cord blood mononuclear cell PBMC and culture of CIK and NK
[0064] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:
[0065] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:
[0066] A freezing medium:
[0067] Human Albumin 14ml
[0068] Cell Stabilizer 6ml
[0069] KBM581 serum-free medium 80ml
[0070] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0071] B cryopreservation solution:
[0072] Medical DMSO 16ml
[0073] KBM581 serum-free medium 84ml
[0074] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0075] Umbilical cord blood mononuclear cell PBMCs are cryopreserved using the above-prepared imm...
Embodiment 3
[0083] Immune cell CIK cryopreservation and recovery
[0084] An immune cell cryopreservation solution and an immune cell cryopreservation method, the specific steps are as follows:
[0085] Prepare immune cell cryopreservation solution, including A cryopreservation solution and B cryopreservation solution, whose components are as follows:
[0086] A freezing medium:
[0087] Human Albumin 20ml
[0088] Cell Stabilizer 10ml
[0089] KBM581 serum-free medium 70ml
[0090] Prepare A freezing solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0091] B cryopreservation solution:
[0092] Medical DMSO 10ml
[0093] KBM581 serum-free medium 90ml
[0094] Prepare B cryopreservation solution according to the above components, and store it in a refrigerator at 4°C for later use;
[0095] The umbilical cord blood mononuclear cells and the CIK cells amplified and cultured by the resuscitated umbilical cord blood mononuclear cells in...
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