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Direct venous re-transfusion immune cell cryopreservation medium and application thereof

A technology of immune cells and cryopreservation solution, applied in the field of biomedicine, which can solve the problems of cumbersome cryopreservation process and subsequent application procedures, unclear components of cryopreservation solution, and unsuitability for large-scale production, so as to facilitate clinical application and ensure safety Effectiveness and effectiveness, good effect of cryopreservation

Active Publication Date: 2017-05-17
HARBIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The technical problem to be solved by the present invention is to overcome the poor cryopreservation effect, unclear composition of cryopreservation solution, relatively cumbersome cryopreservation process and subsequent application procedures, high cost, risky clinical application, and unsuitable scale in the prior art. To overcome defects such as chemical production, provide a direct intravenous infusion of immune cell cryopreservation liquid and its application method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: Preparation and application of immune cell cryopreservation solution for direct intravenous reinfusion

[0034] 1. Raw materials and specifications used

[0035] Hydroxyethyl starch sodium chloride injection: 500ml: 30g of hydroxyethyl starch (200 / 0.5)

[0036] Dextran 40 Glucose Injection: 500ml: 30g Dextran 40 and 25g Glucose

[0037] Heparin sodium injection: 2ml: 12,500 units

[0038] Human albumin injection: 50ml: 10g total protein

[0039] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous reinfusion

[0040] 1) Prepare liquid A and liquid B separately

[0041]Solution A (100ml): 8.3ml dimethyl sulfoxide + 45.8ml hydroxyethyl starch sodium chloride injection + 45.8ml dextran 40 glucose injection + 0.1ml heparin sodium injection.

[0042] The content of each component in liquid A is: dimethyl sulfoxide 8.3v / v%, hydroxyethyl starch 2.75w / v%, dextran 2.75w / v%, glucose 2.29w / v%, heparin sodium 625U / ml, The r...

Embodiment 2

[0056] Embodiment 2: the preparation and application of the immune cell cryopreservation liquid of direct venous reinfusion

[0057] 1. Raw materials and specifications used

[0058] Hydroxyethyl starch sodium chloride injection: 500ml: 30g of hydroxyethyl starch (200 / 0.5)

[0059] Dextran 40 Glucose Injection: 500ml: 30g Dextran 40 and 25g Glucose

[0060] Heparin sodium injection: 2ml: 12,500 units

[0061] Human albumin injection: 50ml: 5g total protein

[0062] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous reinfusion

[0063] 1) Prepare liquid A and liquid B separately

[0064] Solution A (100ml): 10ml dimethyl sulfoxide + 70ml hydroxyethyl starch sodium chloride injection + 19.9ml dextran 40 glucose injection + 0.1ml heparin sodium injection

[0065] The content of each component in liquid A is: dimethyl sulfoxide 10v / v%, hydroxyethyl starch 4.2w / v%, dextran 1.19w / v%, glucose 1.0w / v%, heparin sodium 625U / ml, and the re...

Embodiment 3

[0079] Embodiment 3: the preparation and application of the immune cell cryopreservation liquid of direct venous reinfusion

[0080] 1. Raw materials and specifications used

[0081] Hydroxyethyl starch sodium chloride injection: 500ml: 30g of hydroxyethyl starch (200 / 0.5)

[0082] Dextran 40 Glucose Injection: 500ml: 30g Dextran 40 and 25g Glucose

[0083] Heparin sodium injection: 2ml: 12,500 units.

[0084] Human albumin injection: 50ml: 5g total protein

[0085] 2. Preparation and application of immune cell cryopreservation solution for direct intravenous reinfusion

[0086] 1) Prepare liquid A and liquid B separately

[0087] Solution A (100ml): 9ml dimethyl sulfoxide + 33ml hydroxyethyl starch sodium chloride injection + 57.9ml dextran glucose injection + 0.1ml heparin sodium injection.

[0088] The content of each component in liquid A is: dimethyl sulfoxide 9v / v%, hydroxyethyl starch 1.98w / v%, dextran 3.47w / v%, glucose 2.9w / v%, heparin sodium 625U / ml, and the rest...

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Abstract

The invention discloses a direct venous re-transfusion immune cell cryopreservation medium and application thereof. The direct venous re-transfusion immune cell cryopreservation medium is prepared from dimethyl sulfoxide, human serum albumin, dextran, hydroxyethyl starch, heparin sodium and other clinical medicinal level raw materials by optimizing the proportion of the dose of each raw material. In addition, the operation steps are simplified and the cryopreservation effect is improved by optimizing the cryopreservation method. On one hand, the potential risk of introducing an animal source antigenic substance in the cell treatment process is avoided, and on the other hand, the cryopreservation effect of immune cells is better than that of a conventional cryopreservation medium, thereby guaranteeing the safety and validity in clinical use. Two kinds of components are used for preservation, so that the cryopreservation medium can be preserved stably for a long time. The method disclosed by the invention is simple in operation, provides stronger operability for implementation of large-scale cryopreservation, and simultaneously provides a fundamental technical guarantee for implementation of immune cell treatment.

Description

technical field [0001] The invention relates to a cell cryopreservation solution, in particular to an immune cell cryopreservation solution for direct intravenous infusion and an application method thereof. The invention belongs to the technical field of biomedicine. Background technique [0002] Cancer is the primary problem that plagues human health, and cellular immunotherapy provides broad prospects for the prevention and treatment of tumors. However, in the process of clinical application, there are many problems such as difficulty in collecting autoimmune cells of some tumor patients, poor cell expansion ability, and difficulty in matching the time window between immune cells and other treatment methods; in addition, from the perspective of drug development and production, The off-site transportation, storage conditions and survival time of fresh immune cells are all subject to certain restrictions. Therefore, the effective cryopreservation of immune cells provides t...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0226
Inventor 王振坤周晋曹峰林
Owner HARBIN MEDICAL UNIVERSITY
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