An improved mesenchymal stem cell protection solution and its application
A technology of mesenchymal stem cells and protective solution, which is applied in the field of improved mesenchymal stem cell protective solution, can solve the problems of cell chemical damage and achieve the effects of reducing cell activity loss, improving stress resistance, and reducing accumulation
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Embodiment 1
[0034] An improved mesenchymal stem cell protection solution, comprising the following ingredients in parts by weight:
[0035] 20 parts of osmotic protectant, 20 parts of protein composition, 0.1 part of cell physiological activity inhibitor and 5 parts of amifostine;
[0036] The osmoprotectant includes 12.5 parts of dimethyl sulfoxide, 5 parts of raffinose and 2.5 parts of proline;
[0037] The protein composition includes 2.5 parts of antifreeze protein, 5 parts of γ-globulin and 12.5 parts of plant-derived recombinant human serum albumin;
[0038] The cell physiological activity inhibitor includes 0.05 part of octreotide and 0.05 part of tunicamycin.
Embodiment 2
[0040] An improved mesenchymal stem cell protection solution, comprising the following ingredients in parts by weight:
[0041] 25 parts of osmotic protectant, 15 parts of protein composition, 0.2 part of cell physiological activity inhibitor and 2 parts of amifostine;
[0042] The osmoprotectant includes 12.5 parts of dimethyl sulfoxide, 5 parts of raffinose and 7.5 parts of proline;
[0043] The protein composition includes 2.5 parts of antifreeze protein, 5 parts of gamma-globulin and 7.5 parts of plant-derived recombinant human serum albumin;
[0044] The cell physiological activity inhibitor includes 0.1 part of octreotide and 0.1 part of tunicamycin.
Embodiment 3
[0046] An improved mesenchymal stem cell protection solution, comprising the ingredients described in Example 1, and also including 2 parts by weight of sodium lactated Ringer.
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