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Predicting method of best cooling rate of red blood cell in cryopreservation state

A technology of cooling speed and cryopreservation, which is applied in the field of prediction of the best cooling speed during cryopreservation of red blood cells, can solve problems such as lengthy and cumbersome processes, and achieve the effect of improving the effect of cryopreservation.

Inactive Publication Date: 2012-04-11
UNIV OF SHANGHAI FOR SCI & TECH
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AI Technical Summary

Problems solved by technology

Researchers often use several biochemical indicators to screen out the best experimental plan through different cooling speed experiments. The whole process is cumbersome and lengthy.

Method used

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  • Predicting method of best cooling rate of red blood cell in cryopreservation state
  • Predicting method of best cooling rate of red blood cell in cryopreservation state
  • Predicting method of best cooling rate of red blood cell in cryopreservation state

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Experimental program
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Embodiment 1

[0050] Calorimeter: DSC-Pyris Diamond (Perkin-Elmer, USA)

[0051] Calculation of red blood cell recovery

[0052] Red blood cell recovery rate is the ratio of the number of frozen red blood cells after thawing and washing to the number of cells before freezing.

[0053] RBC % = RBC 1 RBC 2 × 100 %

[0054] In the formula, RBC% is the red blood cell recovery rate (%), RBC 1 is the number of red blood cells (pcs / mL) after the sample is frozen, RBC 2 It is the number of red blood cells (pcs / mL) before the sample is frozen.

[0055] see figure 1 , the present invention discloses a method for predicting the optimal cooling rate of erythrocytes during cryopreservation, that is, differential scanning calorimetry (DSC) predicts the optimal cooling rate of erythrocytes in a specific preservation solution; the method com...

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Abstract

The invention discloses a predicting method of best cooling rate of red blood cell in cryopreservation state, comprising the steps as follows: according to the linear relationship between the heat release of the cell suspension solution during cooling process and the cell volume, ensuring the volume change of the cell in a specific storage solution under constant cooling rate; according to the mathematical model of water transmembrane transport, fitting a percolation parameter corresponding to the red blood cell, simulating the volume changes of the cell under different cooling rates to predict the best cooling rate scope. The cell recovery demonstration tested by the cell cytometer shows that the DSC method can correctly predict the best cooling rate of the cell in the specific storage solution and can greatly reduce the work intensity and is convenient to guide and create a more reasonable low-temperature storing solution.

Description

technical field [0001] The invention relates to a method for predicting the optimal cooling rate, in particular to a method for predicting the optimal cooling rate during cryopreservation of red blood cells. Background technique [0002] At present, blood centers or blood banks generally adopt liquid storage at 4°C, but during the storage period, the ATP and 2,3-DPG contents of the cells will gradually decrease, directly affecting the therapeutic effect, and the storage period is short (only 42 days). Frozen red blood cells are reliable in quality. After thawing and washing, the cell morphology and biochemical characteristics are similar to red blood cells stored in liquid state at 4°C, and the storage period is as long as 10 years. It has become the main storage method for rare blood types and autologous blood transfusion. The demand for blood sources in emergencies such as disasters. [0003] To reduce cryogenic damage to biological materials, it is an urgent problem to d...

Claims

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Application Information

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IPC IPC(8): G01N25/20
Inventor 周新丽袁骥王海松李维杰
Owner UNIV OF SHANGHAI FOR SCI & TECH
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