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Cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof

A nuclear cell and cryopreservation solution technology, which is applied in the field of umbilical cord blood mononuclear cell cryopreservation solution, can solve the problems of affecting the effect of cell therapy, lowering the viability rate, and damaging the cells, so as to achieve improved safety, high viability rate, and improved value effect

Inactive Publication Date: 2018-10-09
吉林省太阳鸟再生医学工程有限责任公司
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AI Technical Summary

Problems solved by technology

[0007] Cell culture medium is also a common component in the current cryopreservation solution formula. Its components are complex and cannot be directly injected into the human body. Therefore, if cells need to be transplanted directly after recovery, the cell culture medium must be removed by centrifugation, and centrifugation will cause The newly recovered cells are damaged and their viability is reduced, which affects the effect of cell therapy

Method used

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  • Cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof
  • Cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof
  • Cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof

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Embodiment 1

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[0058] Mix the above components.

Embodiment 2

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[0061] Mix the above components.

Embodiment 3

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[0065] Mix the above components.

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Abstract

The invention relates to the field of biotechnology, in particular to a cord blood mononuclear cell (CBMC) cryopreservation solution and application thereof. The CBMC cryopreservation solution is prepared from dimethyl sulfoxide (DMSO) combined hydroxyethyl starch (HES), propylene glycol, human albumin and a buffer solution. Compared with a conventional formula, the CBMC cryopreservation solutioncan avoid contamination of animal-derived pathogens, does not take a cell culture medium as a component of a cryoprotectant, and ensures good cell cryopreservation effect. After cryopreservation for 2months, the average cell viability of CBMC cryopreserved with the cryopreservation solution is 97.47% to 98.43%. The recovered cell can be directly used for clinical transplantation, and the CBMC cryopreservation solution is of practical value in clinical application of cell therapy.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a cord blood mononuclear cell cryopreservation solution and its application. Background technique [0002] Cord blood mononuclear cell (CBMC) refers to cells with a single nucleus in cord blood, including hematopoietic stem cells, lymphocytes, monocytes, dendritic cells and a small number of other cells. CBMC can promote the body's hematopoiesis and enhance the body's immunity in vivo; it can induce differentiation into a variety of immune cells in vitro, such as cytokine-induced killer cells (CIK), natural killer cells (NK), and natural killer T cells (NKT) , has important uses in anti-tumor, anti-infection and other aspects. Therefore, cryopreservation of CBMC is of great significance in cell therapy. [0003] Cell cryopreservation is one of the main methods for long-term preservation of cells. cryopreservation of cells in [0004] Low-temperature storage at -196°C in liquid ni...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0221
Inventor 李首一石晓川
Owner 吉林省太阳鸟再生医学工程有限责任公司
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