A microfluidic high-throughput biological sample drop freezing storage device

Abstract

The invention discloses a microfluidic high-throughput biological sample drop freezing storage device, comprising a Stirling refrigerator, a heating table, a sample cover, a microfluidic chip, a first syringe pump, a second syringe pump and a programmable controller , the cold end of the Stirling refrigerator is sleeved with a thermal insulation shell, the heating table is arranged in the thermal insulation shell and is in contact with the cold end of the Stirling refrigerator, the upper surface of the heating table is provided with a microporous sample plate, and the The bottom of the well sample plate is provided with a thermocouple, and the heating table is provided with a heating wire; the sample cover is sealed and sleeved on the top of the heating table, and a sample dripper is movably arranged in the sample cover, and the sample dripper is connected to the microcomputer through a hose. In the fluid control chip, the programmable controller controls the sample dropper to drop the mixed solution into the microwells of the microwell sample plate. In the invention, the process of treating biological samples with the vitrification solution and the dribbling process are controlled by the programmable controller, which realizes automatic preservation, improves the operation efficiency, and avoids possible deviations caused by human operation.

Description

technical field [0001] The invention relates to the technical field of cryopreservation, in particular to a microfluidic high-throughput cryopreservation device for biological samples. Background technique [0002] Low temperature preservation is an effective method for long-term preservation of the activity of biological samples. It refers to cooling live biological samples to a low temperature by a special method, and then rewarming by a special method when necessary, and still obtain live biological samples. The drop freezing method is one of the cryopreservation methods. It drops the vitrification solution containing biological samples on the aluminum foil, and then puts it into liquid nitrogen. Because the cooling is relatively rapid and uniform, it can obtain a higher sample survival rate. At present, the drip freezing method is mostly done manually, which is not only inefficient, but also inevitably leads to inconsistencies in the preservation results. If there is an...

AI Technical Summary

Problems solved by technology

At present, the drop freezing method is mostly done manually, which is not only inefficient, but also inevitably leads to inconsistencies in the preservation results.

If there is an automated drop freezing preservation equipment, the drip freezing preservation can be automated, standardized and batched, so that this method can be more widely used, but there is no such device at present

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