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Embryo production and refrigeration technology

A technology of embryos and crafts, applied in the field of embryo transplantation, can solve the problems of poor embryo effect and low embryo activity, achieve the effects of reducing physical damage, improving activity, and improving cryopreservation effect

Inactive Publication Date: 2018-12-18
内蒙古草原乌骨羊生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But existing embryos are less effective when cryopreserved, and embryos are less viable when thawed and retransferred

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Select donor ewes and rams with excellent genetic characteristics and high breeding value, disease-free, in good health, moderate body condition, and normal reproductive system function, and the donor ewes choose 2-5 litters Ewes, lambing for more than 60 days at the same time, weaning for more than 30 days, the donor ram is 1.5-6 years old, and the sperm quality is good, the sex drive is strong, and the semen of the donor ram is collected, and the quality of the semen is identified Dilute the qualified semen according to the appropriate dilution ratio. Before insemination, the donor ewe needs to fast for 12 hours, and at the same time stimulate the donor ewe in vivo to make superovulation, and use laparoscopy to find the uterine horn of the donor ewe. , and use an insemination gun filled with diluted semen to inject an appropriate amount of semen into the two groups of uterine horns to complete the fertilization;

[0042] On the 2nd to 3rd day after fertilization, the ...

Embodiment 2

[0053] Select donor ewes and rams with excellent genetic characteristics and high breeding value, disease-free, in good health, moderate body condition, and normal reproductive system function, and the donor ewes choose 2-5 litters Ewes, lambing for more than 60 days at the same time, weaning for more than 30 days, the donor ram is 1.5-6 years old, and the sperm quality is good, the sex drive is strong, and the semen of the donor ram is collected, and the quality of the semen is identified Dilute the qualified semen according to the appropriate dilution ratio. Before insemination, the donor ewe needs to fast for 12 hours, and at the same time stimulate the donor ewe in vivo to make superovulation, and use laparoscopy to find the uterine horn of the donor ewe. , and use an insemination gun filled with diluted semen to inject an appropriate amount of semen into the two groups of uterine horns to complete the fertilization;

[0054] On the 5th to 6th day after fertilization, the ...

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PUM

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Abstract

The invention relates to the technical field of embryo transplanting and in particular relates to an embryo production and refrigeration technology, aiming at improving the refrigeration and preservation effect of embryos and improving the activity of the embryos when the embryos are unfrozen and transplanted. The embryo production and refrigeration technology comprises the following steps: (1) selecting a donor ewe and a donor ram; (2) collecting sperm from the donor ram; (3) enabling the donor ewe to superovulate; (4) inseminating; (5) collecting the embryos; (6) identifying the embryos; (7)preparing a solution needed by refrigeration; (8) pre-treating the embryos before refrigeration; (9) filling the embryos into tubes; (10) refrigerating; (11) preserving.

Description

technical field [0001] The invention relates to the technical field of embryo transplantation, in particular to a freezing process for embryo production. Background technique [0002] Embryo transfer, also known as zygote suppression, refers to the technology of transferring early embryos of female animals, or embryos obtained through in vitro fertilization or other methods, into female animals to allow them to continue to develop into new individuals. The essence of embryo transfer is The process of the embryo-producing donor and the embryo-breeding recipient co-producing offspring can give full play to the reproductive ability of excellent female individuals, greatly shorten the reproductive cycle of the donor itself, and increase the number of offspring produced by the donor in its lifetime. [0003] The use of embryo transfer can develop the reproductive potential of female animals with excellent genetic characteristics, and quickly expand the herd of fine breeds. The us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/02A01N1/0221
Inventor 任俊光
Owner 内蒙古草原乌骨羊生物科技有限公司
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