Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Cell seedling temperature cryopreservation method and device

A cell and cryopreservation technology, which is applied to the preservation, application, and animal husbandry of human or animal bodies, can solve the problems of not being able to effectively determine the success or failure of ice planting and the effect, so as to improve the effect of cryopreservation and avoid excessive ice planting or planting. The effect of lack of ice

Pending Publication Date: 2021-08-06
UNIV OF SHANGHAI FOR SCI & TECH
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the problem in the prior art that the success or failure of ice planting and the effect cannot be effectively determined in the process of freezing and storing cells, the purpose of the present invention is to provide a method and device for freezing and storing cells

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Cell seedling temperature cryopreservation method and device
  • Cell seedling temperature cryopreservation method and device
  • Cell seedling temperature cryopreservation method and device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A kind of cell transplantation cryopreservation method, such as figure 1 As shown, the method includes step S1, step S2, step S3, step S4 and step S5.

[0041] Step S1 , cooling down the cryopreservation tube loaded with the cells to be frozen through the refrigeration system according to the preset cooling rate, and detecting the temperature of the cryopreservation tube during the cooling process.

[0042] The refrigeration system is preferably configured as a Stirling refrigerator, which includes a cold end, and cryopreservation tubes loaded with cells to be frozen are arranged at the cold end to obtain cold energy. In addition, a temperature-measuring element, such as a probe of a temperature sensor, is arranged on the freezing tube to detect the temperature of the freezing tube. After the cooling rate is sent to the Stirling refrigerator, the control module mounted on the Stirling refrigerator can control the power of the motor by controlling the duty cycle accordi...

Embodiment 2

[0060] A kind of cell explant cryopreservation device, such as image 3 and Figure 4 As shown, including refrigeration system, ultrasonic ice planting system and control system.

[0061] Among them, the refrigeration system is preferably a Stirling refrigerator. As a mature product, a Stirling refrigerator includes a main body 1 and a cold end 2 arranged on the main body 1, a cooling fan 3, a cooling fin 4 and a The power supply and control module 5 for power supply and control, usually, the main body 1 is surrounded by a casing 6, and the casing 6 is provided with an opening that exposes the power supply and control module 5 so as to facilitate operation. During use, the power supply and control module 5 can pair The cooling rate of the Stirling refrigerator will not be repeated in this embodiment.

[0062] In this embodiment, a freezing platform 7 is provided at the cold end 2, and the freezing platform 7 is made of a material with good thermal conductivity and good ultra...

Embodiment 3

[0067] The difference between it and the second embodiment is that in this embodiment, an insulating layer 12 and an upper cover 13 are also included.

[0068] Wherein the insulation layer 12 is made of insulation material, which is fixed on the cold end of the Stirling refrigerator, and is arranged around the freezing table 7 for a week, thereby protecting the freezing table 7 and preventing external temperature interference.

[0069] Wherein, the upper cover 13 is covered on the top of the insulation layer 12, and it is also made of insulation material.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a cell seedling temperature cryopreservation method and a device, and belongs to the technical field of cell cryopreservation, the method comprises the following steps: S1, cooling a cryopreservation tube loaded with cells to be cryopreserved through a refrigeration system according to a preset cooling rate, and detecting the temperature of the cryopreservation tube in the cooling process; S2, judging whether the temperature of the cryopreservation tube reaches a preset seedling temperature or not, if yes, executing S3, and if not, executing S1 continuesly; S3, adopting the refrigeration system to conduct heat preservation on the cryopreservation tube at the preset seedling temperature; S4, applying ultrasonic waves to the cryopreservation tube through an ultrasonic vibrator so as to carry out seedling temperature operation, and lasting the operation for preset threshold time; S5, judging whether seedling temperature operation of the cryopreservation tube succeeds or not by analyzing the temperature of the cryopreservation tube before and after the seedling temperature operation, if yes, executing S6, and if not, continuing to execute S4; and S6, cooling the cryopreservation tube continuesly through the refrigerating system, stopping cooling after the cryopreservation tube is cooled to the preset cryopreservation temperature, and carrying out heat preservation. According to the method, the cell cryopreservation effect is improved by adding the step of judging whether seedling temperature operation succeeds or not.

Description

technical field [0001] The invention relates to the technical field of cell cryopreservation, in particular to a cell plantation cryopreservation method and device. Background technique [0002] Low temperature preservation is a routine practice for laboratories and sample banks to preserve cells and tissues, but cells and tissues are often damaged during the cooling process. The main factors that cause cell and tissue damage are the toxic damage of cryoprotectants and the intracellular ice damage caused by the formation of intracellular ice during freezing. [0003] Among them, the reason for intracellular ice damage is that during the process of programmed freezing, when the solution temperature is lower than the eutectic temperature but still does not crystallize, the cell solution will be supercooled, and when the supercooling degree is large, it will suddenly nucleate, and then cause Cells are damaged by large ice crystals. [0004] One of the keys to solving intracel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0284A01N1/0278A01N1/0252
Inventor 李维杰申敬刘宝林漆琴姚延禄李中华
Owner UNIV OF SHANGHAI FOR SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com