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Boar seman cryoprotectant

A technology of cryoprotectant and pig semen, which is applied in the field of pig breeding, can solve the problems that pig sperm cannot meet the actual production needs, the survival rate of frozen sperm, the integrity rate of acrosome and plasma membrane, and the damage of sperm, etc. Improve the effect of cryopreservation, ensure fertilization vitality and use effect, and improve the effect of viability

Inactive Publication Date: 2018-01-05
界首市国启家庭农场
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the specificity of porcine sperm cell structure and composition, it is more sensitive to temperature changes, and a large number of sperm are damaged during freezing. The damage of semen and mitochondria is also significantly higher than that of fresh semen, which leads to a general decrease in the pregnancy rate and litter rate of sows in commercial pig production. Therefore, it is difficult to promote the use of frozen semen for artificial insemination in the pig industry. application
Although researchers at home and abroad have done a lot of research on the cryopreservation of pig sperm, the quality of pig sperm after freezing and thawing still cannot meet the actual production needs.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A pig semen cryoprotectant, consisting of the following materials by weight:

[0021] 90 parts of frozen base liquid, 18 parts of fresh egg yolk, 2 parts of glycerin, 0.5 parts of astaxanthin, and 3 parts of Rehmannia glutinosa extract.

[0022] Further, the components in the frozen base liquid and their corresponding parts by weight are: 1 part of glucose, 1.5 parts of citric acid, 2 parts of hyaluronic acid, 0.8 part of trehalose, 0.04 part of penicillin sodium, 0.1 part of streptomycin sulfate, 95 parts double distilled water.

[0023] Further, the preparation method of the Rehmannia glutinosa extract is:

[0024] (1) Put the rehmannia glutinosa into boiling water for 3 minutes, take it out, and then dry it for later use;

[0025] (2) Put the rehmannia after the treatment in step (1) into a casserole filled with wheat bran, heat to keep the temperature of the casserole at 90°C, stir fry continuously for 30 minutes, take it out, filter out the wheat bran and fry the...

Embodiment 2

[0029] A pig semen cryoprotectant, consisting of the following materials by weight:

[0030] 95 parts of frozen base liquid, 20 parts of fresh egg yolk, 4 parts of glycerin, 1 part of astaxanthin, 5 parts of Rehmannia glutinosa extract.

[0031] Further, the components in the frozen base liquid and their corresponding parts by weight are: 1.2 parts of glucose, 1.7 parts of citric acid, 2.2 parts of hyaluronic acid, 1 part of trehalose, 0.05 parts of penicillin sodium, 0.13 parts of streptomycin sulfate, 98 parts double distilled water.

[0032] Further, the preparation method of the Rehmannia glutinosa extract is:

[0033] (1) Put the rehmannia glutinosa into boiling water for 4 minutes, take it out, and then dry it for later use;

[0034] (2) Put the rehmannia after the treatment in step (1) into a casserole filled with wheat bran, heat to keep the temperature of the casserole at 93°C, stir fry continuously for 32 minutes, take it out, filter out the wheat bran and fry the ...

Embodiment 3

[0038] A pig semen cryoprotectant, consisting of the following materials by weight:

[0039] 100 parts of frozen base liquid, 22 parts of fresh egg yolk, 5 parts of glycerin, 1.5 parts of astaxanthin, 6 parts of Rehmannia glutinosa extract.

[0040] Further, the components in the frozen base liquid and their corresponding parts by weight are: 1.5 parts of glucose, 1.8 parts of citric acid, 2.5 parts of hyaluronic acid, 1.2 parts of trehalose, 0.06 parts of penicillin sodium, 0.15 parts of streptomycin sulfate, 100 parts double distilled water.

[0041] Further, the preparation method of the Rehmannia glutinosa extract is:

[0042] (1) Put the rehmannia glutinosa into boiling water for 5 minutes, take it out, and then dry it for later use;

[0043] (2) Put the Shudi treated in step (1) into a casserole filled with wheat bran, heat to keep the temperature of the casserole at 95°C, stir fry continuously for 35 minutes, take it out, filter out the wheat bran and stir fry Shudi f...

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PUM

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Abstract

The invention discloses a boar seman cryoprotectant which is prepared from the following substances: cryopreservation basic liquid, fresh egg yolk, glycerinum, astaxanthin and a radix rehmanniae preparata extract. The boar seman cryoprotectant is subjected to special matching treatment; under the common action of all the components, the finally prepared cryoprotectant obviously enhances the boar seman cryopreservation effect, and increases the boar seman viability, acrosomal integrity and film integrity rate; the fertilization vitality and the use effect of boar semans are guaranteed to the maximum extent.

Description

technical field [0001] The invention belongs to the technical field of pig breeding, and in particular relates to a cryoprotectant for pig semen. Background technique [0002] Semen cryopreservation technology is developed along with artificial insemination technology, which is a major innovation in artificial insemination technology. The principle of semen cryopreservation technology is to use liquid nitrogen (-196°C), dry ice (-79°C) or other cold sources to properly process the sperm to completely stop sperm metabolism. Thaw artificial insemination and in vitro fertilization when used. Semen freezing can greatly increase the utilization rate of breeding males, reduce the amount of breeding males, save costs and is not limited by region and time. It is beneficial to the hybrid improvement of livestock breeds and is an effective technical means for livestock breeding and protection of endangered species. In a word, the cryopreservation of semen is of great significance t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 赵国启
Owner 界首市国启家庭农场
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