Composite super-living body S staining solution

Abstract

The invention discloses a composite super-living body S staining solution, and relates to the technical field of urine detection staining, in particular to the composite super-living body S staining solution which comprises the following raw materials: 1-20mmol / L of alcian blue; 8 to 25 mmol / L of acid blue; 1 to 20 mmol / L of piperidine B; 10 to 35 mmol / L of sand yellow; the concentration of the MES Buffer is 0.1 mol / L. The composite super-living body S staining solution can only stain urine visible components while inhibiting staining of impurities in urine, and can reduce background interference and effectively inhibit mixing of impurities during urinary sediment detection by inhibiting staining of urine sediments during urinary sediment detection, so that a method capable of effectively improving the detection rate of target cells is provided, and the detection efficiency of the target cells is improved. Furthermore, when urine sediment is detected and analyzed, it is possible to sensitively detect rare components such as renal tubular epithelial cells and casts, and to perform a highly sensitive urinary sediment test by increasing the rate of detection of rare components such as epithelial cells and casts in a urine sample. The present invention enables highly accurate analysis of visible components in a urine sample using an automatic analyzer.

Description

technical field [0001] The invention relates to the technical field of urine detection and dyeing, in particular to a composite super living body S staining solution. Background technique [0002] Urine formed component examination is a very important, non-invasive urine morphological examination, which has little burden on the subjects and the specimens are easy to obtain. Microscopic examination of urine formed components, usually using unstained microscopy. However, the repeatability of this operation is poor, the missed detection rate is relatively high, and some formed components are difficult to identify. There are many known methods for staining biological components. Among them, the method of staining cells by injecting a dye into a living body is called "biological staining", and the method of staining cells taken from a living body in a living state is called "biological staining". The method is called "vitality staining". [0003] In the existing urine sediment...

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Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the present invention provides a composite super-living S staining solution, which solves the problem that not only the cells need to be measured, but also some problems that may exist in the urine are solved in the existing urine sediment inspection proposed in the above-mentioned background technology. Impurities, stained impurities will interfere with the background, making it difficult to detect target cells, especially when using a formed component analyzer for urine sediment inspection, if the impurities appearing in the background are stained, they may interfere with the imaging The components to be tested overlap, which makes it difficult to classify or incorrectly classify, making it difficult for the automatic urine analyzer to accurately identify the formed components. In addition, the traditional urine sediment inspection and staining method, in addition to impurities are easy to be stained, there will also be renal tubular epithelial cells It is difficult to dye rare components such as tubes and casts, resulting in a low detection rate of rare components and the problem of missed detection