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Fluorescent staining solution and application thereof

A technology of fluorescent dyeing and fluorescent agent, applied in the field of fluorescent dyeing, can solve the problems such as easy missed diagnosis or misdiagnosis of microorganisms, small size of mold blastospores, difficult identification of microorganisms, etc., and achieve the effect of simplified detection process, short time consumption, and easy identification.

Pending Publication Date: 2021-03-09
SHENZHEN ZIJIAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of the related technologies mentioned above, the inventor believes that the magnification of the traditional direct smear method is relatively low, and the mold blastospores are small in size, which is easy to be misidentified, and when the slide is dirty, the microorganisms are more difficult to identify. As a result, it is easy to miss or misdiagnose microbiological testing

Method used

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  • Fluorescent staining solution and application thereof
  • Fluorescent staining solution and application thereof
  • Fluorescent staining solution and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1 discloses a fluorescent dyeing solution, which is made of the following raw materials: fluorescent agent, buffer solution, and preservative. The fluorescent staining solution also includes purified water, add purified water to make the system 1L. Wherein, the buffer solution includes 8.2g of acetic acid, the rest is sodium acetate, and the preservative is Proclin 300.

[0060] Weigh the above-mentioned raw materials according to the proportion, add each raw material into water, stir and mix evenly, and obtain the fluorescent dyeing solution.

Embodiment 2-5

[0062] Embodiments 2-5 disclose a fluorescent dyeing solution, the types of raw materials are the same as those in Embodiment 1, the difference lies in the ratio of raw materials, and the specific ratio is shown in Table 2.

Embodiment 6-12

[0064] Embodiments 6-12 disclose a fluorescent staining solution, the types of raw materials are shown in Table 1, and the ratios of raw materials are shown in Table 2.

[0065] Table 1 The raw material ratio of the fluorescent staining liquid of embodiment 1-12

[0066]

[0067] The kind of raw material of the fluorescent staining solution of table 2 embodiment 1-12

[0068]

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Abstract

The invention relates to the technical field of fluorescent staining, and particularly discloses a fluorescent staining solution and an application thereof. The fluorescent staining solution is prepared from the following raw materials: a fluorescent agent, a buffer solution, a preservative and water. The fluorescent staining solution disclosed by the invention can be used for staining epithelialcells, leukocytes, bacteria, fungi and parasites of human secretions or body fluids, and has the advantages of improving a microbiological detection rate and reducing a missed diagnosis rate and a misdiagnosis rate.

Description

technical field [0001] This application relates to the technical field of fluorescent dyeing, more specifically, it relates to a fluorescent dyeing solution and its application. Background technique [0002] Pathogenic microorganisms are a class of microorganisms that can invade the human body and cause infections or even infectious diseases, also known as pathogens. The family of pathogens is vast and includes bacteria, fungi, parasites, viruses, and more. [0003] Different pathogenic microorganisms invade and infect the human body and easily cause different diseases. For example, diarrhea-causing Escherichia coli easily disturbs the balance of the human intestinal tract and causes diarrhea; after Salmonella invades human epithelial cells, it will cause nausea and vomiting symptoms in the human body, causing enteritis ; Hemolytic streptococci multiply in the human respiratory tract in large quantities, which will cause sore throat and fever, and suffer from tonsillitis; ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/28
CPCG01N1/30G01N1/2813G01N2001/302
Inventor 马力敏杨海芳赵起卿
Owner SHENZHEN ZIJIAN BIOTECH
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