819results about How to "Promote recombination" patented technology

The present invention provides compositions and methods for targeted cleavage of cellular chromatin in a region of interest and / or homologous recombination at a predetermined site in cells. Compositions include fusion polypeptides comprising a TAL effector binding domain and a cleavage domain. The cleavage domain can be from any endonuclease. In certain embodiments, the endonuclease is a Type IIS restriction endonuclease. In further embodiments, the Type IIS restriction endonuclease is FokI.

The invention relates to compound epoxy flooring coating adopting the mixture of epoxide resin and modified resin as a base material and further comprising organic solvent, dispersant, wetting agent, antifoaming agent, flatting agent, scratch resistance agent, pigment and stuffing as well as reactive diluent selected according to requirements. When in use, the coating is mixed and solidified withthe components of firming agent, accelerant, etc. By regulating the content of the components, the compound epoxy flooring coating of the invention can be used for manufacturing a solvent or solvent-free autolevelling epoxy floor. The compound epoxy flooring coating of the invention has favorable over-all properties, and not only has the characteristics of high mechanical strength, favorable anti-corrosion property, high adhesive force and good wear resistance property of the epoxide resin but also has the characteristics of good weather resistance, high decorative property and hardness and shock resistance of the modified resin; and the preparation method thereof is simple and practicable, is low-cost, and is suitable for mass preparation.

The invention relates to a method for preparing endogenetic particle reinforced metal matrix composites under the coupling effect of a magnetic field and supersonic waves. The method comprises: adjusting the temperature of a metal matrix fused mass to the reaction initial temperature after refining, adding a reactant which can generate a particle phase with the fused mass through the reaction in situ for implementing the synthesis reaction, standing the mixture after the reaction is over, and cooling the mixture to the pouring temperature for pouring; and the method is characterized in that: the magnetic field and the high-intensity ultrasonic field are simultaneously exerted in the reaction synthesis process to realize the synthesis of endogenetic particle reinforced metal matrix composites under the coupling effect of the magnetic field and the high-intensity ultrasonic field. The magnetic field can be an intense pulse magnetic field, a high frequency oscillation magnetic field or a low frequency alternating magnetic field. In the method, the coupling effect of the magnetic field and the ultrasonic field makes the particle sizes be thinned and dispersed evenly; the ultrasonic vibration agitation and electromagnetic stirring functions improve the composite dynamic condition, and the compounding of the particle phase and a base metal interface is better; and the co-action of magnetochemistry and sonochemistry improves the thermodynamic condition of the reaction in situ, as well as speeding up the reaction in situ and controlling the growth of the particle phase.

The invention discloses a preparation method of a soft and durable anti-penetrating material. The preparation method is characterized by comprising the following steps: (1) preparing shearing thickening liquid; (2) preparing a high performance fiber fabric with a special tissue structure, namely preparing a fabric from high performance fibers by virtue of a special tissue structure weaving process, putting the fabric into a vacuum drying oven, and drying at 120-150 DEG C for 2-3 hours; and (3) preparing the anti-penetrating material. According to the method, by virtue of reasonable tissue structure configuration on the fabric and plasma treatment on the shearing thickening liquid and the fabric, the shearing thickening liquid and the fabric are adequately compounded, so that the durabilityand flexibility of the anti-penetrating material are improved, and the soft and durable anti-penetrating material suitable for multiple environments of daily protective clothes of military police, armored military equipment, civil and industrial protection and the like is prepared.

PROBLEM TO BE SOLVED: To provide a production method for a thermoplastic resin composition, composed of a polyphenylene ether and a polyamide, having an electric conductivity, and excellent in balance of fluidity and impact strength, by producing with a specific process, and to provide the thermoplastic resin composition obtained thereby. SOLUTION: The thermoplastic resin composition is composed of the following component (A)-(E), wherein a weight ratio of (A) / (B) is 5 / 95-70 / 30, an amount of (C) is effective to phase-solvate (A) and (B), the amount of (D) is 0.8-10 pts.wt. per the total 100 pts.wt. of (A) and (B), and the amount of component (E) is 5-40 pts.wt. per the total 100 pts.wt. of (A) and (B). The production method for the thermoplastic resin composition is provided. (A): a polyphenylene ether, (B): a polyamide. (C): a phase-solvating agent, (D): an electrically conductive carbon black and / or a fine fibrous carbon, and (E) an improving agent for shock resistance.

The invention discloses a preparation method of organic-chain-segment-modified graphene oxide. According to the invention, graphite is oxidized under the effects of strong oxidizing agents such as concentrated sulfuric acid, sodium nitrate and potassium permanganate; the oxidized graphite is subjected to ultrasonic dispersion in an organic solvent, such that a graphene oxide dispersion system is prepared; diisocyanate and dihydric alcohol are subjected to a reaction; hydroxyacrylate is used for sealing one end; and an NCO group in the organic chain segment is subjected to a reaction with the prepared graphene oxide, such that the organic-chain-segment-modified graphene oxide is prepared. The organic chain segment contains polymerizable double bonds, such that cross-linking polymerization of the modified graphene oxide and other organic materials can be promoted, and composite materials with special properties can be obtained.

The invention provides a house interlayer cast-in-situ cavity floor. The house interlayer cast-in-situ cavity floor comprises interlayer space height reserved in a main body house building, light steel beams, a light steel beam and wall slab combined member, a reinforced concrete upper flange slab, a hollow ribbed steel mesh and a lower flange thin slab compounded with concrete mortar at the bottom of the hollow ribbed steel mesh, wherein a house interlayer cast-in-situ cavity floor is arranged in the reserved space height of the main body building; the house interlayer cast-in-situ cavity floor adopts light steel beams; the compounded lower flange thin slab is directly compounded by cement mortar and the bottom surface of the hollow ribbed steel mesh; the thickness of the lower flange thin slab is smaller than or equal to 30mm; the hollow ribbed steel mesh comprises a hollow mesh, a floor end mesh and a support mesh; the thickness of the interlayer cast-in-situ cavity floor is smaller than or equal to 250mm. Through the house interlayer cast-in-situ cavity floor, the defect that any filling body cannot act and stress with the floor of the existing cavity floor hole formation technique is overcome; the potential safety hazard of the quality caused by non-uniform house interlayer standard is reduced; the interlayer durability co-exists with the building main body.

The instant invention is directed toward a dermal delivery system composition comprising an aqueous base vehicle including Emu oil, at least one fatty acid alkyl ester, polyethylene glycol, and a gelling agent, in combination with a therapeutically effective amount of at least one species of insulin, and to processes for the manufacture and use thereof.

The present invention discloses a graphene / CNTs / Super-P composite electric conduction agent, a composite electric conduction agent slurry, and preparation methods of the graphene / CNTs / Super-P composite electric conduction agent and the composite electric conduction agent slurry, wherein the composite electric conduction agent comprises graphene, CNTs (carbon nano-tubes) and Super-P (electric conduction carbon black). The preparation method comprises: adding a graphene oxide slurry, carbon nano-tubes, electric conduction carbon black and a surfactant to a reaction kettle, uniformly stirring, carrying out complete ultrasonic dispersion, drying, crushing, adding the crushed mixture to a muffle furnace, puffing in air, carrying out a reduction treatment in an inert atmosphere, and cooling to a room temperature to obtain the composite electric conduction agent powder, wherein the composite electric conduction powder can further be mixed with a solvent, and stirring and ultrasonic dispersion are performed to prepare the composite electric conduction agent slurry. According to the present invention, the composite electric conduction agent has excellent performances, and is suitable for being used as the electric conduction agent of power ion batteries; and the used preparation process has characteristics of simpleness and low energy consumption, and is suitable for large-scale industrial production.

The invention relates to a preparation method of a ZnO, CuO and ZnS quantum dot film. The preparation method comprises the following steps: preparing Cu ion modified ZnO quantum dot precipitate, CuO quantum dot precipitate and ZnS quantum dot precipitate; adding trichloromethane in the prepared quantum dot precipitates respectively to fully dissolve, then adding n-octylamine dispersant to obtain Cu ion modified ZnO colloidal quantum dot solution, CuO colloidal quantum dot solution and ZnS colloidal quantum dot solution; diluting the three kinds of colloidal quantum dot solutions with the sameamount of trichloromethane, filtering with a polytetrafluoroethylene (PTFE) filter, coating one or more of the colloidal quantum dot solutions on an indium-tin oxide (ITO) substrate through the spin-coating method, prebaking, and annealing in the air to obtain the quantum dot single-layer or multi-layer film. The preparation process of the invention is simple; the coated quantum dot film is compact and smooth and has good adhesion performance and controllable film thickness; and the quantum dot multi-layer structure is easy to realize and the quantum dot film is suitable for the multi-layer quantum dot film element.

The invention belongs to the technical field of microcapsule self-repair materials and particularly relates to a double-shell epoxy resin microcapsule self-repair material and a preparation method thereof. Epoxy resin serves as a repairing agent core material of the microcapsule self-repair material. Firstly, a single-shell microcapsule self-repair material with melamine-urea-formaldehyde resin asan inner wall material is prepared according to an in-situ polymerization method; then, a sol-gel method is adopted for preparing the double-layer microcapsule self-repair material with an inorganicSiO2 polymer as an outer wall material. Compared with a traditional emulsifying agent, graphene oxide serving as an epoxy resin emulsifying agent has advantages of nontoxicity and environmental friendliness. The inorganic SiO2 polymer with excellent biocompatibility and resistance to acids and alkalis serves as the outer wall material to improve compatibility of the microcapsule self-repair material to cement concrete materials, and the microcapsule self-repair material is enabled to give a full play to the self-repair performance in the cement concrete materials.