209 results about "Etioplasts" patented technology

The present invention relates generally to the amphiphilic polyelectrolyte, poly(2-ethylacrylic acid) and covalently bonded lipids to generate Lipo-PEAA. These Lipo-PEAA are then used to make pH-sensitive liposomes which become unstable, permeable or fusogenic with certain pH changes. In addition, this invention generally describes methods for delivering therapeutic compounds and drugs to target cells by administering to a host the pH-sensitive liposomes of the present invention.

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

The invention relates to a targeting personalized DC cell membrane bionic liposome drug carrier as well as a preparation method and application thereof. The DC cell membrane bionic liposome drug carrier is characterized in that (1) the liposome drug carrier has a cell membrane protein component; (2) the liposome drug carrier can carry a drug in vitro and is used for cell targeting fused release; and (3) the cell membrane protein component of the liposome comes from immortalized somatic cells. The invention has the following advantages: the drug carrier can be applied to the targeting delivery to in-vivo DC cells of a drug or antigen, the antigen presentation ability of the DC cells is enhanced, and the specific immunity is improved.

Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.

The invention discloses a method for separating and converting rape protoplast. The method comprises the following steps: 1, selecting rape cotyledons or true leaves, and removing lower epidermis; 2, putting into enzymolysis liquid for carrying out enzymolysis, wherein the enzymolysis liquid is prepared from the following formula components: 1wt% of cellulase, 0.3wt% of macerozyme, 0.5M of mannitol, 20mM of KCl, 20nM of MES with a pH value of 5.7, 10mM of CaCl2 and 0.1wt% of BSA; 3, adding an isovolumetric W5 solution into a mixed solution obtained by means of enzymolysis, carrying out low speed centrifugation for removing supernatant, adding W5 for washing and resuspending, adding resuspended liquid into 20wt% of a sucrose solution for purifying, horizontally centrifuging, and taking supernatant green protoplast liquid; 4, centrifuging for removing the W5 solution, and resuspending protoplast precipitate by using an MMG solution; 5, converting the protoplast. The protoplast separating and converting method is established in rape for the first time; the rape protoplast is used as a receptor for converting, so that yellow fluorescent protein (YFP), luciferase (Luc) and renilla reniformis luciferase (Ren) are successfully expressed.

The invention relates to a method for the production of plants with suppressed photo-respiration and improved CO2 fixation. In particular, the invention relates to a re-use of phosphoglycolate produced in photorespiration. The reaction product will be converted to a component that may be reintegrated into the plant assimilatory metabolism inside the chloroplast. This is accomplished by the transfer of genes derived from glycolate-utilizing pathways from bacteria, algae, plants and / or animals including humans into the plant nuclear and / or plastidial genome. The method of the invention leads to a reduction of photorespiration in C3 plants and by this will be of great benefit for food production especially but not exclusively under non-favourable growth conditions.

Vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery are provided. The invention provides edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis.

A composition for administration of a therapeutically effective dose of a topoisomerase inhibitor I or topoisomerase I / II inhibitor is described. The composition includes liposomes having an outer surface and an inner surface defining an aqueous liposome compartment, and being composed of a vesicle-forming lipid and of a vesicle-forming lipid derivatized with a hydrophilic polymer to form a coating of hydrophilic polymer chains on both the inner and outer surfaces of the liposomes. Entrapped in the liposomes is the topoisomerase inhibitor at a concentration of at least about 0.10 μmole drug per μmole lipid.

The invention relates to a flurbiprofen liposome and a preparation method thereof. The flurbiprofen liposome is prepared by weighting the following materials in part by weight: 1 to 10 parts of cholesterol, 5 to 40 parts of yolk lecithin, 2 to 50 parts of flurbiprofen and 1 to 10 parts of vitamin C; dissolving the materials with chloroform and methanol in a mass ratio of 4:1; uniformly mixing the materials to obtain mixed solution; putting the solution into a rotary evaporator; distilling the solution under reduced pressure for removing the chloroform and the methanol to obtain a lipid membrane; adding phosphate buffer with pH between 6 and 8 into the lipid membrane; rotating for 1 to 4 h in warm bath at the temperature of between 20 and 60 DEG C on the rotary evaporator; and carrying out water bath ultrasound for 5 to 30 min, and then filtering the mixture with a 0.22 to 0.45 mu m filter membrane to obtain the flurbiprofen liposome. The flurbiprofen liposome prepared by the invention has good stability, targeting property and slow-releasing property, can reduce administration dosage and reduce toxic and side effect of medicaments, is suitable for percutaneous administration and oral administration, and can be widely used for preparing oral liquid, aerosol, spray, and ophthalmic and external liposome administration formulations.

The invention discloses a nano-liposome containing cell penetrating peptide. The nano-liposome is prepared from lecithin, a surface active agent, vitamin E and the cell penetrating peptide in the weight ratio of (10-60) to (10-30) to (0.1-10) to (0.2-20); and the cell penetrating peptide is selected from at least one of biological source cell penetrating peptide, chimeric cell penetrating peptideand artificially synthesized cell penetrating peptide. According to a preparation method of the nano-liposome, a brand-new high pressure homogenization dispersion method is utilized, the stability ofthe nano-liposome is greatly improved, and the nano-liposome can be applied to a tank-mix assistant or a fly prevention assistant agriculturally. The nano-liposome containing the cell penetrating peptide has the high stability, can be stored for a long time at room temperature, is not sensitive to changes of pH value and ionic strength, and has good compatibility with pesticides and / or fertilizers. No organic solvents are needed in the preparation process, and no pollution is generated. The nano-liposome can be transmitted fast in vivo after being applied to crops, and reaches the minimum effective drug concentration rapidly, prolongs the persistence time, improves the prevention and treatment effect and delays occurrence of drug resistance.