213 results about "Etioplasts" patented technology

Disclosed is a means of controlling plant pests by a novel method of expressing Cry2Ab B. thuringiensis delta-endotoxins in plants, targeted to the plastids. The invention comprises novel nucleic acid segments encoding proteins comprising Cry2Ab B. thuringiensis delta-endotoxins. The nucleic acid segments are disclosed, as are transformation vectors containing the nucleic acid segments, plants transformed with the claimed segments, methods for transforming plants, and methods of controlling plant infestation by pests.

Various embodiments provide, for example, vectors, expression cassettes, and cells useful for transgenic expression of nucleic acid sequences. In various embodiments, vectors can contain plastid-based sequences of unicellular photosynthetic bioprocess organisms for the production of food- and feed-stuffs, oils, biofuels, pharmaceuticals or fine chemicals.

The present invention relates generally to the amphiphilic polyelectrolyte, poly(2-ethylacrylic acid) and covalently bonded lipids to generate Lipo-PEAA. These Lipo-PEAA are then used to make pH-sensitive liposomes which become unstable, permeable or fusogenic with certain pH changes. In addition, this invention generally describes methods for delivering therapeutic compounds and drugs to target cells by administering to a host the pH-sensitive liposomes of the present invention.

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

The invention relates to a multi-vesicular liposome, a blank multi-vesicular liposome and a preparation method and application thereof. The multi-vesicular liposome contains the following components in part by weight: 1 part of liposome, 0.01 to 20 parts of auxiliary emulsifier, 1 to 50 parts of osmotic pressure regulator and medicinal active ingredients; the medicament-to-lipid ratio of the multi-vesicular liposome is 1:(0.1-1):200; the lipid contains of a specific amount of neutral phospholipid, cholesterol and triglyceride; and the auxiliary emulsifier is selected from dextran, polyvinyl pyrrolidone, hydroxyethyl starch, gelatin, albumin, arginine and hydroxymethyl starch. The blank multi-vesicular liposome contains the following components in part by weight: 1 part of lipid, 0.01 to 20 parts of auxiliary emulsifier, 1 to 50 parts of osmotic pressure regulator and 0.1 to 50 parts of ion gradient regulator; the osmotic pressure of the in vivo water phase of the multi-vesicular liposome is equal to the osmotic pressure of human plasma; and the auxiliary emulsifier is as previously mentioned. The multi-vesicular liposome has high entrapment rate, and can achieve good sustained-release effect on in vivo and in vitro experiments.

The invention provides methods and compositions for obtaining transplastomic Arabidopsis plants. Specifically, the method provides culturing protocols and compositions that facilitate the regeneration of transformed plants following delivery of exogenous DNA molecules.

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

The invention discloses a Bdellovibrio strain for preventing and treating mastitis and applications thereof. A Bdellovibrio BDS01 is obtained by separation, and is unicellular, arced and 0.9-0.25 micrometer in size; the end of the Bdellovibrio BDS01 has flagellum which is 4 micrometers long. When cultured by a two-layer plating method at 28 DEG C for four days, the Bdellovibrio BDS01 can form transparent round plaques with the diameter of 1-2 mm. The microbial preparation prepared by using the Bdellovibrio BDS01 plastid has favorable effects for preventing cow mastitis. Compared with the telotroch, the plastid has the characteristics of convenient preparation and storage, strong environmental resistance, slightly slow sterilization action, longer sterilization action time, and the like, and does not have toxic or side effect on cows. Therefore, the microbial preparation containing the Bdellovibrio BDS01 plastid is suitable for large-size factory culture of cows, and a novel method forgreen processing and production of milk and for preventing and treating mastitis can be provided.

The invention provides indocyanine green nanometer targeted liposome. The indocyanine green nanometer targeted liposome comprises indocyanine green, a phospholipid layer and a folic acid-modified polyethylene glycol derived phospholipid layer, wherein the indocyanine green is surrounded by the phospholipid layer; the folic acid-modified polyethylene glycol derived phospholipid layer comprises polyethylene glycol derived phospholipid and folic acid connected with the polyethylene glycol derived phospholipid through an amido bond; phospholipid in the polyethylene glycol derived phospholipid is inserted in the phospholipid layer; the folic acid is exposed outside the phospholipid layer; the mass ratio of the indocyanine green to the phospholipid to the folic acid-modified polyethylene glycol derived phospholipid is (1-5): (1-3): (1-3). The invention further provides a preparation method of the indocyanine green nanometer targeted liposome and photo-thermal treating application thereof; when the indocyanine green nanometer targeted liposome is applied, the indocyanine green is difficultly gathered, and has excellent stability and a targeting property.

The invention discloses a method for performing transient expression by introducing a foreign gene into a poplar bioplast. The method comprises the following steps of: introducing a transient expression vector containing a foreign gene into a poplar bioplast by using PEG (Polyethylene Glycol)-Ca<2+>; culturing; and expressing the foreign gene in the bioplast. A poplar bioplast transient expression system constructed with the method is easy to operate and realize, a place with broad prospects is opened up for the research of functional genes of a poplar, and the transformation efficiency of the method can be up to 70 percent.

This disclosure concerns compositions and methods for targeting peptides, polypeptides, and proteins to plastids of plastid-containing cells. In some embodiments, the disclosure concerns chloroplast transit peptides that may direct a polypeptide to a plastid, and nucleic acid molecules encoding the same. In some embodiments, the disclosure concerns methods for producing a transgenic plant material (e.g., a transgenic plant) comprising a chloroplast transit peptide, as well as plant materials produced by such methods, and plant commodity products produced therefrom.

Disclosed are the uses of specific genes of the mevalonate and isoprenoid biosynthetic pathways, and of inactive gene sites (the pseudogene) to (1) enhance biosynthesis of isopentenyl diphosphate, dimethylallyl diphosphate and isoprenoid pathway derived products in the plastids of transgenic plants and microalgae, (2) create novel antibiotic resistant transgenic plants and microalgae, and (3) create a novel selection system and / or targeting sites for mediating the insertion of genetic material into plant and microalgae plastids. The specific polynucleotides to be used, solely or in any combination thereof, are publicly available from GeneBank and contain open reading frames having sequences that upon expression will produce active proteins with the following enzyme activities: (a) acetoacetyl CoA thiolase (EC 2.3.1.9), (b) 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) synthase (EC 4.1.3.5), (c) HMG-CoA reductase (EC 1.1.1.34), (d) mevalonate kinase (EC 2.7.1.36), (e) phosphomevalonate kinase (EC 2.7.4.2), (f) mevalonate diphosphate decarboxylase (EC 4.1.1.33), (g) isopentenyl diphosphate (IPP) isomerase (EC 5.3.3.2), and (h) phytoene synthase (EC 2.5.1.32).

The invention provides thermo-sensitive liposome. The thermo-sensitive liposome comprises a chemotherapeutic drug, a photosensitizer, phosphatidylcholine and polyethylene glycol-derived phospholipid, wherein the photosensitizer is indocyanine green or chlorins e6; the grain size of the thermo-sensitive liposome is 40-60 nm; by virtue of living-body fluorescence imaging, a distribution process of the thermo-sensitive liposome can be observed in real time; under a near-infrared light irradiation condition, the chemotherapeutic drug can be quickly and effectively released, so that the chemotherapeutic drug can be enriched at a target part very well; the obtained thermo-sensitive liposome particle has smaller particle diameter less than 100 nm, and good biocompatibility. The preparation method of the thermo-sensitive liposome is simple and easy to operate.

The invention discloses a preparation method of tea polyphenol nano-liposomes by combination of an ethanol injection method and dynamic high-pressure microfluidization-enzymolysis. Tea polyphenol, lecithin, tween-80 and a phosphate buffer solution are adopted as raw materials. The tea polyphenol nano-liposomes are prepared by utilization of an ethanol injection-dynamic high-pressure microfluidization-enzymolysis method. According to the prepared tea polyphenol nano-liposomes, the average particle size is 67.4 nm plus or minus 3.0 nm, the zeta potential is -6.07 mV plus or minus 0.59 mV, the distribution coefficient is 0.220 plus or minus 0.010, and the encapsulation efficiency is 79.7% plus or minus 5.4%. The prepared tea polyphenol nano-liposomes show good slow-release property. Only 30.3% plus or minus 2.9% of the tea polyphenol is released after 24 h. In a small-intestine simulation environment where pH is 7.4, the tea polyphenol nano-liposomes show good stability.

The invention discloses a method for preparing and regenerating a phomopasis asparagi protoplast, which relates to the technical field of fungus protoplast preparation and regeneration in cell engineering. The method comprises the following detailed operation steps of: (1) preparing a phomopasis asparagi conidium suspension; (2) preparing a fresh mycelium; (3) performing enzymolysis on mycelium cell walls; (4) separating the protoplast; and (5) regenerating the protoplast. The method is easy to operate, low in requirements on equipment and short in enzymolysis time and regeneration period, and effectively enhances the efficiency of preparation and regeneration of the protoplast.

The invention relates to a targeting personalized DC cell membrane bionic liposome drug carrier as well as a preparation method and application thereof. The DC cell membrane bionic liposome drug carrier is characterized in that (1) the liposome drug carrier has a cell membrane protein component; (2) the liposome drug carrier can carry a drug in vitro and is used for cell targeting fused release; and (3) the cell membrane protein component of the liposome comes from immortalized somatic cells. The invention has the following advantages: the drug carrier can be applied to the targeting delivery to in-vivo DC cells of a drug or antigen, the antigen presentation ability of the DC cells is enhanced, and the specific immunity is improved.

Vaccines for conferring immunity in mammals to infective pathogens are provided, as well as vectors and methods for plastid transformation of plants to produce protective antigens and vaccines for oral delivery. The invention further provides transformed plastids having the ability to survive selection in both the light and the dark, at different developmental stages by using genes coding for two different enzymes capable of detoxifying the same selectable marker, driven by regulatory signals that are functional in proplastids as well as in mature chloroplasts. The invention utilizes antibiotic-free selectable markers to provide edible vaccines for conferring immunity to a mammal against Bacillus anthracis, as well as Yersina pestis. The vaccines are operative by parenteral administration as well. The invention also extends to the transformed plants, plant parts, and seeds and progeny thereof. The invention is applicable to monocot and dicot plants.

The invention relates to a ginkgolide B nanoliposomes drug and a method for preparing the same. The invention provides the ginkgolide B nanoliposomes drug including the following materials and the following radios: 8-12 mol compositions of cephalin and distearoyl choline with mole ratio of 1:3-6; 6-8 mol compositions of sojasterol and cholesterin with mole ratio of 1:2-4; 4-6 mol compositions of gamma-cyclodextrin / 2-hychoxypropyl-beta-cyclodextrin and alpha-cyclodextrin with mole ratio of 3:1; 0.2-0.4 mol methoxy polyethylene glycol 2000-hydrogenated soybean phosphatidylethanolamine; 2 mol ginkgolide B; and 0.1-0.5 mol vitamine E. A method for preparing the ginkgolide B nanoliposomes drug is also provided in the invention to produce the ginkgolide B nanoliposomes for target treating blocking of vessel or endothelial cell thereof, with a better healing effect than ordinary preparations of ginkgolide B.

The invention discloses a method for separating and converting rape protoplast. The method comprises the following steps: 1, selecting rape cotyledons or true leaves, and removing lower epidermis; 2, putting into enzymolysis liquid for carrying out enzymolysis, wherein the enzymolysis liquid is prepared from the following formula components: 1wt% of cellulase, 0.3wt% of macerozyme, 0.5M of mannitol, 20mM of KCl, 20nM of MES with a pH value of 5.7, 10mM of CaCl2 and 0.1wt% of BSA; 3, adding an isovolumetric W5 solution into a mixed solution obtained by means of enzymolysis, carrying out low speed centrifugation for removing supernatant, adding W5 for washing and resuspending, adding resuspended liquid into 20wt% of a sucrose solution for purifying, horizontally centrifuging, and taking supernatant green protoplast liquid; 4, centrifuging for removing the W5 solution, and resuspending protoplast precipitate by using an MMG solution; 5, converting the protoplast. The protoplast separating and converting method is established in rape for the first time; the rape protoplast is used as a receptor for converting, so that yellow fluorescent protein (YFP), luciferase (Luc) and renilla reniformis luciferase (Ren) are successfully expressed.

The invention relates to a non-cleavable, plastidial targeting polypeptide derived from a protein from the inner membrane of plant chloroplasts. Said peptide is particularly suitable for importing proteins of interest in plants.

The invention relates to a flurbiprofen liposome and a preparation method thereof. The flurbiprofen liposome is prepared by weighting the following materials in part by weight: 1 to 10 parts of cholesterol, 5 to 40 parts of yolk lecithin, 2 to 50 parts of flurbiprofen and 1 to 10 parts of vitamin C; dissolving the materials with chloroform and methanol in a mass ratio of 4:1; uniformly mixing the materials to obtain mixed solution; putting the solution into a rotary evaporator; distilling the solution under reduced pressure for removing the chloroform and the methanol to obtain a lipid membrane; adding phosphate buffer with pH between 6 and 8 into the lipid membrane; rotating for 1 to 4 h in warm bath at the temperature of between 20 and 60 DEG C on the rotary evaporator; and carrying out water bath ultrasound for 5 to 30 min, and then filtering the mixture with a 0.22 to 0.45 mu m filter membrane to obtain the flurbiprofen liposome. The flurbiprofen liposome prepared by the invention has good stability, targeting property and slow-releasing property, can reduce administration dosage and reduce toxic and side effect of medicaments, is suitable for percutaneous administration and oral administration, and can be widely used for preparing oral liquid, aerosol, spray, and ophthalmic and external liposome administration formulations.

A method for constructing a recombinant double-expression silkworm polyhedral baculiform virus which contains the SOD gene comprises that the general RNA of the silkworm is extracted and an MnSOD primer is designed; a single-stranded cDNA is synthesized and an MnSOD gene is synthesized; the MnSOD gene is connected with a p FastBacHTa to transform XL-Blue cells; and the recombinant donor plasmid p FastBacHTa / MnSOD can be extracted; the baculiform virus DNA of the silkworm is taken as the template to synthesize the silkworm polyhedral gene and the gene is cloned to the p FastBac Dual plasmid; the MnSOD gene is inserted under the P10 promoter of the double-expression plasmid to construct the double-expression vector: Bm polyhedrin pFB dual / MnSOD; after the transinfection of the Bm polyhedrin pFB dual / MnSOD, the double-expression virus DNA which contains the MnSOD gene and the polyhedral gene is distilled; the DNA is transduced into the silkworm culture cell of transinfection with the mediation of transinfection reagent lipidosome to obtain the recombinant virus. Virus particles which can feed and infect the silkworm is provided and the object gene MnSOD is expressed by the silkworm and large-scale and industrial production is realized.

The present invention provides a cationic phospholipid liposome composition comprising 1,2-dioleoyl-sn-glycero-S-ethylphosphocholine (EDOPC), 3β-[N—(N′,N′-dimethylaminoethane)-carbamoyl] cholesterol (DC-cholesterol) and 1,2-diphytanoyl-sn-glycero-3-phosphoethanolamine (DPhPE), a liposome-nucleic acid complex which is capable of forming a complex therewith, and a pharmaceutical composition comprising the same. The cationic phospholipid liposome of the present invention is highly effective for intracellular delivery of nucleic acids and reduction of cytotoxicity, as compared to conventional liposome products. Therefore, the present invention can be useful for gene therapy via intracellular delivery of a desired material to target cells.

The invention relates to a preparation method and application of a 20 (R)-ginsenoside Rg3 / soya bean lecithin / cholesterol / folic acid lipidosome medicine and belongs to the field of medicine health care products.By optimizing the ratio of soya bean lecithin, cholesterol and an encapsulation medicine to obtain 20 (R)-ginsenoside Rg3 lipidosome, the grain diameters of the 20 (R)-ginsenoside Rg3 lipidosome are reduced, and meanwhile the stability of the lipidosome medicine is enhanced; folic acid is used for conducting target modification on the encapsulated 20 (R)-ginsenoside Rg3 medicine lipidosome, and then target medicine delivery of cancer cells is achieved.Experimental results show that the encapsulation rate of the 20 (R)-ginsenoside Rg3 lipidosome can be up to 90% or above.The preparation method of the composite lipidosome medicine is simple and efficient.The prepared composite lipidosome is low in cytotoxicity, has the slow release feature and is capable of prolonging the curative effect, safe, efficient and high in targeting, good evaluating effects are obtained in physical representation and extracorporeal biological study, and a new approach is provided for research and development of anti-cancer medicines.

Boron neutron capture therapy can utilize XyB20H17L where X is an alkali metal, y is 1 to 4, and L is a two electron donor such as NH3, and Na2B10H9NCO, among others. These borane salts may be used free or encapsulated in liposomes. Liposomes may also have embedded within their bilayers carboranes to increase the amount of delivered 10B and / or to increase the tumor specificity of the liposome.

The invention discloses a nano-liposome containing cell penetrating peptide. The nano-liposome is prepared from lecithin, a surface active agent, vitamin E and the cell penetrating peptide in the weight ratio of (10-60) to (10-30) to (0.1-10) to (0.2-20); and the cell penetrating peptide is selected from at least one of biological source cell penetrating peptide, chimeric cell penetrating peptideand artificially synthesized cell penetrating peptide. According to a preparation method of the nano-liposome, a brand-new high pressure homogenization dispersion method is utilized, the stability ofthe nano-liposome is greatly improved, and the nano-liposome can be applied to a tank-mix assistant or a fly prevention assistant agriculturally. The nano-liposome containing the cell penetrating peptide has the high stability, can be stored for a long time at room temperature, is not sensitive to changes of pH value and ionic strength, and has good compatibility with pesticides and / or fertilizers. No organic solvents are needed in the preparation process, and no pollution is generated. The nano-liposome can be transmitted fast in vivo after being applied to crops, and reaches the minimum effective drug concentration rapidly, prolongs the persistence time, improves the prevention and treatment effect and delays occurrence of drug resistance.