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Indocyanine green nanometer targeted liposome as well as preparation method and application thereof

A technology targeting liposomes and indocyanine green, applied in the field of nanomaterials, can solve the problems of lack of tumor cell targeting specificity, limit the application of indocyanine green, and short cycle time, so as to avoid decomposition and clearance in vivo , The preparation method is simple and easy to operate, and the effect of improving targeting

Active Publication Date: 2014-04-02
珠海中科先进技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These Defects greatly limit the application of indocyanine green

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  • Indocyanine green nanometer targeted liposome as well as preparation method and application thereof
  • Indocyanine green nanometer targeted liposome as well as preparation method and application thereof
  • Indocyanine green nanometer targeted liposome as well as preparation method and application thereof

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preparation example Construction

[0049] In a second aspect, the present invention provides a method for preparing indocyanine green nano-targeted liposomes, comprising the following steps:

[0050] (1) Dissolving indocyanine green, phospholipids and folic acid-modified polyethylene glycol derivatized phospholipids in a solvent in a mass ratio of (1~5):(1~3):(1~3) to obtain the first mixture solution, the folic acid-modified polyethylene glycol derivatized phospholipid layer comprises polyethylene glycol derivatized phospholipids and folic acid linked to the polyethylene glycol derivatized phospholipids through an amide bond;

[0051] (2) removing the solvent in the first mixed solution by rotary evaporation to obtain a film-shaped material;

[0052] (3) Dissolving the film-like material and ultrasonicating for 1-10 minutes to obtain a second mixed solution;

[0053] (4) Extruding the second mixed solution through a polycarbonate membrane with a pore diameter of 200nm for 3 to 8 times, and then extruding thro...

Embodiment 1

[0076] A preparation method for indocyanine green nano-targeted liposomes, comprising the following steps:

[0077] (1) Dissolve indocyanine green, soybean lecithin and FA-PEG-DSPE (PEG molecular weight is 3350) in a mixed solution of 3 mL of chloroform and methanol at a mass ratio of 1:1.7:1.7 to obtain the first mixed solution ; FA-PEG-DSPE includes polyethylene glycol derivatized phospholipids and folic acid linked to polyethylene glycol derivatized phospholipids through amide bonds;

[0078] (2) removing chloroform and methanol in the first mixed solution by rotary evaporation to obtain a film-like material;

[0079] (3) Add 2.5mL of ultrapure water to dissolve the film-like material, and use an ultrasonic cell disruptor to sonicate for 2min at a frequency of 20kHz and a power of 130W to obtain the second mixed solution;

[0080](4) Extrude the second mixed solution through a polycarbonate membrane with a pore size of 200nm for 5 times, and then squeeze through a polycarb...

Embodiment 2

[0084] A preparation method for indocyanine green nano-targeted liposomes, comprising the following steps:

[0085] (1) Dissolve indocyanine green, cephalin, and folic acid-modified FA-PEG-DSPE (PEG molecular weight is 5000) in a mixed solution of 3 mL of acetonitrile and water at a mass ratio of 1:1.7:1.7:2 to obtain the first Mixed solution; FA-PEG-DSPE includes polyethylene glycol derivatized phospholipids and folic acid linked to polyethylene glycol derivatized phospholipids through amide bonds;

[0086] (2) Remove acetonitrile and water by rotary evaporation to obtain a film-like material;

[0087] (3) Add 2.5 mL of PBS buffer solution to dissolve the membrane-like material, and use an ultrasonic cell disruptor to sonicate for 2 minutes at a frequency of 20 kHz and a power of 130 W to obtain a second mixed solution;

[0088] (4) Extrude the second mixed solution through a polycarbonate membrane with a pore size of 200nm for 3 times, and then squeeze through a polycarbona...

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Abstract

The invention provides indocyanine green nanometer targeted liposome. The indocyanine green nanometer targeted liposome comprises indocyanine green, a phospholipid layer and a folic acid-modified polyethylene glycol derived phospholipid layer, wherein the indocyanine green is surrounded by the phospholipid layer; the folic acid-modified polyethylene glycol derived phospholipid layer comprises polyethylene glycol derived phospholipid and folic acid connected with the polyethylene glycol derived phospholipid through an amido bond; phospholipid in the polyethylene glycol derived phospholipid is inserted in the phospholipid layer; the folic acid is exposed outside the phospholipid layer; the mass ratio of the indocyanine green to the phospholipid to the folic acid-modified polyethylene glycol derived phospholipid is (1-5): (1-3): (1-3). The invention further provides a preparation method of the indocyanine green nanometer targeted liposome and photo-thermal treating application thereof; when the indocyanine green nanometer targeted liposome is applied, the indocyanine green is difficultly gathered, and has excellent stability and a targeting property.

Description

technical field [0001] The invention belongs to the field of nanomaterials, and in particular relates to an indocyanine green nano-targeted liposome, a preparation method thereof and an application of photothermal therapy. Background technique [0002] Indocyanine Green (ICG) is a tricarbonate hydrogen dye, its maximum absorption wavelength and maximum emission wavelength are in the near-infrared region of 740nm and 800nm, and it has strong fluorescence penetration. It is a reagent approved by the Food and Drug Administration (FDA) for human medical imaging and diagnosis, and it is used as a photothermal preparation for clinical hyperthermia. However, indocyanine green will quickly aggregate in polar solvents, and its stability is poor. It will be quickly cleared in plasma, and the circulation time in the body is short. At the same time, indocyanine green also lacks tumor cell targeting specificity. These The defect greatly limits the application of indocyanine green. Con...

Claims

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Application Information

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IPC IPC(8): A61K9/127A61K41/00A61K47/34A61P35/00
Inventor 蔡林涛郑明彬赵鹏飞罗震宇龚萍郑翠芳
Owner 珠海中科先进技术研究院有限公司
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