Blood detection micro-fluidic chip

Abstract

The invention discloses a blood detection micro-fluidic chip. The blood detection micro-fluidic chip comprises a substrate and a cover plate; and the cover plate and the substrate are in sealing fit to form a chip body; the chip body is provided with a plurality of separation and detection units, and the plurality of separation and detection units are radially distributed by taking the circle center of the chip body as an original point; each separation and detection unit comprises a whole blood sampling pool, a plasma separation pool, an erythrocyte sedimentation pool, a plasma storage pool, a quantification pool, a front primary waste liquid pool, a front secondary waste liquid pool, a rear primary waste liquid pool, a detection reagent sampling pool, a detection pool, a whole blood sample injection hole, a whole blood sample injection pool vent hole, a plasma storage pool vent hole, a rear primary waste liquid pool vent hole and a detection reagent sample injection hole, wherein the whole blood sampling pool, the plasma separation pool, the erythrocyte sedimentation pool, the plasma storage pool, the quantification pool, the front primary waste liquid pool, the front secondary waste liquid pool, the rear primary waste liquid pool, the detection reagent sampling pool and the detection pool are arranged on the substrate, and the whole blood sample injection hole, the whole blood sample injection pool vent hole, the plasma storage pool vent hole, the rear primary waste liquid pool vent hole and the detection reagent sample injection hole are formed in the cover plate. The blood detection micro-fluidic chip has separation and detection functions at the same time, can achieve plasma / serum separation, reagent injection, reaction and detection integration; and the problem that blood coagulation measurement and detection cannot meet the ever-increasing instant detection requirement is solved.

Description

technical field [0001] The invention relates to the technical fields of molecular detection and microfluidic chips, and more specifically relates to a blood detection microfluidic chip. Background technique [0002] The coagulation system of the human body is a rather complex system, including coagulation factors and anticoagulation factors, fibrinolytic system and antifibrinolytic system, etc. [0003] Coagulation detection is of great significance for disease prevention, disease treatment and blood transfusion therapy. However, at present, there are still problems in blood sample storage, sample extraction and pretreatment, detection efficiency and detection timeliness in coagulation detection. The main limitations are: [0004] 1. Blood samples should not be stored for a long time, and the risk of detection index changes and degradation will easily occur over time; [0005] 2. Blood coagulation testing requires cumbersome sample extraction and pretreatment processes, an...

AI Technical Summary

Problems solved by technology

However, at present, there are still problems in blood sample storage, sample extraction and pretreatment, detection efficiency and detection timeliness in coagulation detection. The main limitations are:

[0004] 1. Blood samples should not be stored for a long time, and the risk of detection index changes and degradation will easily occur over time;

[0005] 2. Blood coagulation testing requires cumbersome sample extraction and pretreatment processes, and each step needs to be equipped with fixed instruments and consumables, which are prone to problems such as operational errors and sample contamination, which affect the reliability of testing;

[0006] 3. The traditional blood coagulation test separates the blood sample from the test, each step takes a certain amount of time, the test efficiency is low, and timely or batch testing cannot be achieved;

[0007] 4. There is a large demand for blood samples for traditional testing, but the amount of blood sample collection is limited

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