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Blood collection card and application thereof

A blood collection and plasma technology, applied in the field of blood collection cards, can solve the problems of low plasma recovery rate, red blood cells clogging the filter membrane, etc.

Pending Publication Date: 2022-04-29
浙江嘉孚生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Patent CN107430114B discloses a plasma separation card, which uses vertical diafiltration to separate blood cells and plasma, and there is a risk of red blood cells clogging the filter membrane, resulting in a low plasma recovery rate

Method used

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  • Blood collection card and application thereof
  • Blood collection card and application thereof
  • Blood collection card and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] First, prepare red blood cell separation pad 2 according to the requirements. The material used is a polyester fiber pad, which is the PT-R5 model of the MID brand, and the mouse anti-human red blood cell antibody (purchased from Xiamen Wanbo Biotechnology Co., Ltd., article number: ABRBC001) is mixed with normal saline. Dilute to 1 mg / ml, immerse the PT-R5 polyester fiber pad in the treatment solution, and after fully infiltrating, dry it for 8 hours at room temperature in an environment with humidity less than 30%, to complete the preparation of red blood cell separation pad 2. The white blood cell storage pad 3 uses an asymmetric polyethersulfone membrane (purchased from Hangzhou Corbetter Filter Material Co., Ltd., item number: PSM0180-B). Plasma Preservation Pad 4 Use cellulose filter paper (such as Whatman TM 903 TM ), in this embodiment, the cellulose filter paper is fully infiltrated with a nucleic acid preservation solution (purchased from Beijing Dawen Biotec...

Embodiment 2

[0049] Use the basic component 7 of the blood collection card prepared in Example 1 to add 150 μl of whole blood sample to verify the effect of blood separation, such as Figure 8 Shown on the left. At the same time, the blood separation effect of the blood collection card basic component 7 prepared by using the cell separation pad 2 without mouse anti-human erythrocyte treatment was compared, as Figure 8 Shown on the right. exist Figure 8 In the left picture, it can be clearly observed that red blood cells are aggregated and trapped on the red blood cell separation pad 2, while Figure 8 The red blood cells in the right picture are trapped by the white blood cell preservation pad 3; while Figure 8 In the left figure, the recovery rate of plasma is significantly higher than that of Figure 8 Plasma recovery in the middle right panel. This embodiment illustrates the beneficial effect of this patent on the separation of components of whole blood and the recovery of plasm...

Embodiment 3

[0051] The blood collection card prepared in Example 1 was tested for its ability to preserve nucleic acid in plasma.

[0052] Sample preparation:

[0053] A1: Use fresh whole blood samples from healthy people, add the new crown genome nucleic acid, prepare whole blood samples positive for the new crown nucleic acid, and drop 150 μl / well into the sample well 5 of the blood collection card prepared in formula 1 of the embodiment of the present invention, Place it at room temperature for 4 hours, then use tweezers and other tools to remove the plasma storage pad 4, put it into a 1.5ml conical centrifuge tube, add 500μl nucleic acid extraction reagent, vortex fully for about 1min; perform subsequent nucleic acid extraction and PCR detection, and detect CT Value, repeated in three holes, respectively A1-1, A1-2, A1-3;

[0054] A2: Use fresh whole blood samples from healthy people, add the new crown N gene pseudovirus, prepare N gene pseudovirus positive whole blood samples, and d...

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Abstract

The invention provides a blood collection card and application thereof, and belongs to the technical field of sample collection devices.The blood collection card comprises a blood collection card basic component and a bottom plate; the blood collection card basic component comprises a rubber plate; a red blood cell separation pad, a white blood cell preservation pad and a blood plasma preservation pad are sequentially adhered to the rubber plate in a mutual overlapping manner; or the red blood cell separation pad and the plasma preservation pad are sequentially adhered to the rubber plate in a mutual overlapping manner; the blood collection card basic component is fixed on the bottom plate; the red blood cell separation pad comprises a red blood cell antibody and / or lectin; the leukocyte preservation pad is made of a hydrophilic filter membrane, and the aperture of the hydrophilic filter membrane is 0.5-5 microns. According to the method, the lateral chromatography effect of blood on the surface of the membrane is fully utilized, red blood cells can be separated out, interference of the red blood cells on leukocytes and / or plasma is eliminated, and effective separation and preservation of the leukocytes and / or plasma are achieved.

Description

technical field [0001] The invention belongs to the technical field of sample collection devices, and in particular relates to a blood collection card and its application. Background technique [0002] Blood consists of plasma and blood cells. In addition to a large amount of water, plasma also contains inorganic salts, fibrinogen, albumin, globulin, enzymes, hormones, various nutrients, and metabolites. In addition to these, when the human body is invaded by viruses, there will be a large amount of viruses and nucleic acids in the plasma of the human body, so plasma is the most important sample type for disease detection. There are three types of blood cells: red blood cells, white blood cells, and platelets. [0003] Red blood cells have no nucleus, no genetic material, and are usually not used as target substances for disease detection. Because red blood cells themselves have a very dark color and are large in number, accounting for about 45% of the overall blood volume...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00C12N5/078C12Q1/6806C12N15/10
CPCC12M33/00C12M33/14C12N5/0641C12N5/0634C12Q1/6806C12N15/1003C12N2509/00C12Q2527/125
Inventor 漆新国贺然
Owner 浙江嘉孚生物科技有限公司
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