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Bacteria capable of removing organic matter and ammonia nitrogen in micro-polluted water source water under low temperature and aerobic condition, and screening and taming method

A water source water and micro-pollution technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problems of environmental secondary pollution, unsuitable treatment, accumulation of nitrite and nitrate, etc.

Inactive Publication Date: 2009-08-12
HARBIN INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the phenomenon of nitrite and nitrate accumulation in the effluent and the production of N 2 O Causes secondary pollution to the environment and is not suitable for the treatment of micro-polluted water source water and low-temperature treatment, and provides bacteria that can simultaneously remove organic matter and ammonia nitrogen in micro-polluted water source water under low temperature and aerobic conditions and screening and domestication methods

Method used

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  • Bacteria capable of removing organic matter and ammonia nitrogen in micro-polluted water source water under low temperature and aerobic condition, and screening and taming method

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specific Embodiment approach 1

[0011] Specific Embodiment 1: In this embodiment, the bacteria SRA10 that can simultaneously remove organic matter and ammonia nitrogen in micro-polluted water source water under low temperature and aerobic conditions is Acinetobacter lwoffii, which belongs to the genus Acinetobacter (Acinetobacter), and has been established in China as a microbial bacterium Preserved by the General Microorganism Center of Species Preservation Management Committee, the preservation number is CGMCC No.2889, and the preservation date is January 19, 2009; the bacteria that can simultaneously remove organic matter and ammonia nitrogen in slightly polluted source water under low temperature and aerobic conditions are Gram-negative Aerobic bacteria, spherical or short rod-shaped, 1.5 μm to 2.5 μm in length and 1.0 μm to 1.5 μm in width, without spores and flagella, and twitching; form milky white colonies on beef extract peptone medium, and the colonies are round , the surface of the colony is raised...

specific Embodiment approach 2

[0013] Specific embodiment two: this embodiment is different from specific embodiment two in that it can simultaneously remove organic matter and ammonia nitrogen bacteria in slightly polluted water source water under low temperature and aerobic conditions, the contact enzyme is positive, and the oxidase is negative; the methyl red test is negative, no Produces acetylmethylmethanol; does not produce H 2 S, does not hydrolyze starch, does not liquefy gelatin; produces indole; the growth pH is 6-8, and the growth temperature is 2-30°C. Others are the same as in the second embodiment.

specific Embodiment approach 3

[0014]Specific implementation method three: this embodiment can simultaneously remove organic matter and ammonia nitrogen bacteria in slightly polluted water source water under low temperature and aerobic conditions. The enrichment solution was obtained after enrichment and cultivation under 170r / min aerobic conditions for 48 hours, and the enrichment solution was serially diluted and spread on LB solid medium, separated and cultured for 48 hours under the condition of 28-32°C, and then picked different characteristics. The single colonies were inoculated on LB solid medium for purification and culture for 24 hours; 2. The purified strains were inoculated in LB liquid medium and cultured for 24 hours at 28-32°C to obtain bacterial liquid; 3. Take Inoculate 1ml of the bacterial liquid into the screening medium, and cultivate it at 28-32°C for 48 hours; 4. Take 1ml of the bacterial liquid of the surviving strains and transfer it to a fresh screening medium, and cultivate it at 28...

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Abstract

The invention discloses bacteria capable of synchronously removing organic substances and ammonia nitrogen in water of a slightly polluted water source under low-temperature and aerobic conditions and a screening and domesticating method, which relate to bacteria and a screening and domesticating method and solve the problem that the prior bacteria are unsuitable for treating the slightly polluted water source as well as low temperature treatment. The bacteria SRA10 of the invention are acinetobacter lwoffi which belong to acinetobacter baumannii and are preserved in China General Microbiological Culture Collection Center on January 19th, 2009, with a preservation number of CGMCC No.2889. The screening method comprises the following steps: I, separating and purifying; II, preparing bacterial solution; III, screening and cultivating a bacterial solution; IV, screening and cultivating a bacterial solution with a live strain; V, repeating the step IV for 1 to 2 times, and stepwise domesticating the live strain; and VI, taking a bacterial solution of the domesticated strain, and rescreening the bacterial solution to obtain the bacteria. The bacteria obtained by screening and domesticating can process water of the slightly polluted water source under a condition of a low temperature of 2 to 10 DEG C.

Description

technical field [0001] The invention relates to a bacterium and a method for screening and acclimating it. Background technique [0002] Ammonia nitrogen (NH 3 -N) as ionic ammonia (NH 4 + ) and non-ionic ammonia (NH 3 ) exist in two forms in water. Ammonia chlorinated in drinking water exceeding 0.2 mg / L will cause smell and taste problems, and will also reduce the disinfection effect. Ammonia in the water pipe will be nitrous bacteria to generate nitrite, which will cause harm to human health. Refractory organic matter in drinking water is difficult to degrade, and is easy to amplify its harm through food chain bioaccumulation. [0003] With the deepening of research on heterotrophic nitrification-aerobic denitrification, a large number of heterotrophic nitrifying bacteria have been isolated, such as Peseudomonas spp, Alcaligenes faecalis, Thiosphaera pantotropha, Thauera mechernichensis, etc. However, during the treatment of the above strains, the accumulation of ni...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F3/02C12R1/01
CPCY02W10/10
Inventor 李伟光张多英解丰波郜玉楠王广智
Owner HARBIN INST OF TECH
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