Tissue cultivation method of Fritillaria cirrhosa D.Don

Abstract

The invention discloses a tissue cultivation method of bulbus fritillariae cirrhosae. Fritillaria cirrhosa D.Don is a Liliaceae Fritillaria perennial medicinal herb, underground bulbs are used as medicine, the bulbus fritillariae cirrhosae has the effects of clearing away heat and moistening the lungs, preventing phlegm from forming and stopping coughing, and is one of the main sources for the famous traditional Chinese medicinal material bulbus fritilariae specified in the Chinese Pharmacopoeia (Year 2005). The bulbus fritillariae cirrhosae is distributed in high altitude localities, is propagated through seeds, and is low in reproductive rate and long in growth cycle; due to long-term harvesting and wild resource deficiency, the problem of large market demand needs to be solved urgently. Bulbs of the bulbus fritillariae cirrhosae are used as explants, bulbus fritillariae cirrhosae in-vitro regenerated plants are obtained through the processes of explant sterilization, induction of callus tissues in two different states, bulb differentiation, multiplication, rooting culture, acclimatization and transplantation and the like, an effective tissue culture rapid propagation system is established, a large number of seedlings can be produced in a short period of time, the breeding efficiency is improved, and the propagation and popularization of the bulbus fritillariae cirrhosae are facilitated.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a method for tissue culture of Fritillaria cirrhosa D.Don. Background technique [0002] Fritillaria cirrhosa D.Don is a perennial herbal medicinal plant of the genus Fritillaria in the family Liliaceae. Its medicinal parts are underground bulbs. It has the effects of clearing away heat and nourishing the lungs, reducing phlegm and relieving cough. It is an important medicine for treating cough. Chuan Fritillaria, Dark Purple Fritillaria (Fritillaria unibracteata Hsiaoet K.C.Hsia), Gansu Fritillaria (Fritillariaprzewalskii Maxim), Suosha Fritillaria (FritillariadelavayiFranch.) are the same as the famous Chinese medicinal materials Chuanbei in the "Chinese Pharmacopoeia" (2005). main source. Chuan Fritillaria is mainly produced in Sichuan, Tibet, Yunnan and other provinces and regions. It likes cold and cool climate conditions. It has the characteristics of cold resistance, humidity...

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Problems solved by technology

In the existing patents, the tissue culture of Fritillaria has been reported, but the number is small, and the varieties involved are Dark Purple Fritillaria, Hubei Fritillaria, Yili Fritillaria, Flat Fritillaria, Yellow Flower Fritillaria, Anhui Fritillaria, Gansu Fritillaria Among them, there are fewer reports about Fritillaria cirrhosa D.Don, and Wang Yuehua et al. have carried out callus induction studies with the roots of Fritillaria cirrhosa D.Don as explants

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